Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L
The total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from explants (cotyledons, petioles, hypocotyls and leaves) obtained from Medicago arborea L. seedlings were evaluated. Total sugars were the major components in the calli and no significant differences be...
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| Veröffentlicht in: | Plant science (Limerick) 2000-05, Vol.154 (2), p.143-151 |
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| container_title | Plant science (Limerick) |
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| creator | Martin, Ana Belen Cuadrado, Yolanda Guerra, Hilario Gallego, Piedad Hita, Oscar Martin, Luisa Dorado, Ana Villalobos, Nieves |
| description | The total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from explants (cotyledons, petioles, hypocotyls and leaves) obtained from
Medicago arborea L. seedlings were evaluated. Total sugars were the major components in the calli and no significant differences between embryogenic and non-embryogenic calli were observed. In contrast, important differences between the embryogenic and non-embryogenic calli were observed for reducing sugars, the highest levels being observed in embryogenic calli. The highest starch levels were found in non-embryogenic calli developed in MS medium. During the development of somatic embryogenesis very low starch levels in the callus were found. During the first months of culture, no significant differences in the sucrose content were found between calli that produced embryos and those that did not. The most important differences in sucrose were seen between calli transferred to medium F0, which had the greatest embryogenic capacity, and those transferred to medium F6, which inhibited embryogenesis. In the latter case, an increase in sucrose was observed. |
| doi_str_mv | 10.1016/S0168-9452(99)00251-4 |
| format | Article |
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Medicago arborea L. seedlings were evaluated. Total sugars were the major components in the calli and no significant differences between embryogenic and non-embryogenic calli were observed. In contrast, important differences between the embryogenic and non-embryogenic calli were observed for reducing sugars, the highest levels being observed in embryogenic calli. The highest starch levels were found in non-embryogenic calli developed in MS medium. During the development of somatic embryogenesis very low starch levels in the callus were found. During the first months of culture, no significant differences in the sucrose content were found between calli that produced embryos and those that did not. The most important differences in sucrose were seen between calli transferred to medium F0, which had the greatest embryogenic capacity, and those transferred to medium F6, which inhibited embryogenesis. In the latter case, an increase in sucrose was observed.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/S0168-9452(99)00251-4</identifier><identifier>PMID: 10729613</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Biological and medical sciences ; Biotechnology ; Embryogenic calli ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; In vitro embryo culture ; Medicago arborea ; Metabolism ; Methods. Procedures. Technologies ; Non-embryogenic calli ; Photosynthesis, respiration. Anabolism, catabolism ; Plant cells and fungal cells ; Plant physiology and development ; Reducing sugars ; Starch ; Sucrose ; Total sugars</subject><ispartof>Plant science (Limerick), 2000-05, Vol.154 (2), p.143-151</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-ca98a3ff77ed019357c62fd20cd4af12619815d8029e23abb21dc5a61f8d4f873</citedby><cites>FETCH-LOGICAL-c444t-ca98a3ff77ed019357c62fd20cd4af12619815d8029e23abb21dc5a61f8d4f873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945299002514$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1427290$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10729613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martin, Ana Belen</creatorcontrib><creatorcontrib>Cuadrado, Yolanda</creatorcontrib><creatorcontrib>Guerra, Hilario</creatorcontrib><creatorcontrib>Gallego, Piedad</creatorcontrib><creatorcontrib>Hita, Oscar</creatorcontrib><creatorcontrib>Martin, Luisa</creatorcontrib><creatorcontrib>Dorado, Ana</creatorcontrib><creatorcontrib>Villalobos, Nieves</creatorcontrib><title>Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>The total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from explants (cotyledons, petioles, hypocotyls and leaves) obtained from
Medicago arborea L. seedlings were evaluated. Total sugars were the major components in the calli and no significant differences between embryogenic and non-embryogenic calli were observed. In contrast, important differences between the embryogenic and non-embryogenic calli were observed for reducing sugars, the highest levels being observed in embryogenic calli. The highest starch levels were found in non-embryogenic calli developed in MS medium. During the development of somatic embryogenesis very low starch levels in the callus were found. During the first months of culture, no significant differences in the sucrose content were found between calli that produced embryos and those that did not. The most important differences in sucrose were seen between calli transferred to medium F0, which had the greatest embryogenic capacity, and those transferred to medium F6, which inhibited embryogenesis. In the latter case, an increase in sucrose was observed.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Embryogenic calli</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In vitro embryo culture</subject><subject>Medicago arborea</subject><subject>Metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Non-embryogenic calli</subject><subject>Photosynthesis, respiration. Anabolism, catabolism</subject><subject>Plant cells and fungal cells</subject><subject>Plant physiology and development</subject><subject>Reducing sugars</subject><subject>Starch</subject><subject>Sucrose</subject><subject>Total sugars</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkc9uFSEUxomxsdfqI2hYuKiJU4GBmWFlTP2b3MaFuiYMHG4xM1CBMelj9I1l7r2p3bk5J_n4nQN8H0IvKLmghHZvv9cyNJILdi7la0KYoA1_hDZ06NuGMSEfo809coqe5vyLrJTon6BTSnomO9pu0N0H7xwkCAYy9gGXa8AmhgKhZBwdLrHoCedlp1N-gxPYxfiwuxdy0clcYx1slUyKGdYlMI_pNu4geLM_CjE0DzWjp8ljl-KMr8B6o3cR6zTGBBpvn6ETp6cMz4_9DP389PHH5Zdm--3z18v328ZwzktjtBx061zfgyVUtqI3HXOWEWO5dpR1VA5U2IEwCazV48ioNUJ31A2Wu2rSGTo_7L1J8fcCuajZZwPTpAPEJSs6CNnSrhVDRcUBXX-YEzh1k_ys062iRK1pqH0aarVaSan2aShe514er1jGGeyDqYP9FXh1BHSuprikg_H5H8dZBUnF3h0wqH788ZBUNn6NzPoEpigb_X9e8hc0uqhs</recordid><startdate>20000529</startdate><enddate>20000529</enddate><creator>Martin, Ana Belen</creator><creator>Cuadrado, Yolanda</creator><creator>Guerra, Hilario</creator><creator>Gallego, Piedad</creator><creator>Hita, Oscar</creator><creator>Martin, Luisa</creator><creator>Dorado, Ana</creator><creator>Villalobos, Nieves</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000529</creationdate><title>Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L</title><author>Martin, Ana Belen ; Cuadrado, Yolanda ; Guerra, Hilario ; Gallego, Piedad ; Hita, Oscar ; Martin, Luisa ; Dorado, Ana ; Villalobos, Nieves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-ca98a3ff77ed019357c62fd20cd4af12619815d8029e23abb21dc5a61f8d4f873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Embryogenic calli</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In vitro embryo culture</topic><topic>Medicago arborea</topic><topic>Metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Non-embryogenic calli</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Plant cells and fungal cells</topic><topic>Plant physiology and development</topic><topic>Reducing sugars</topic><topic>Starch</topic><topic>Sucrose</topic><topic>Total sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martin, Ana Belen</creatorcontrib><creatorcontrib>Cuadrado, Yolanda</creatorcontrib><creatorcontrib>Guerra, Hilario</creatorcontrib><creatorcontrib>Gallego, Piedad</creatorcontrib><creatorcontrib>Hita, Oscar</creatorcontrib><creatorcontrib>Martin, Luisa</creatorcontrib><creatorcontrib>Dorado, Ana</creatorcontrib><creatorcontrib>Villalobos, Nieves</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martin, Ana Belen</au><au>Cuadrado, Yolanda</au><au>Guerra, Hilario</au><au>Gallego, Piedad</au><au>Hita, Oscar</au><au>Martin, Luisa</au><au>Dorado, Ana</au><au>Villalobos, Nieves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2000-05-29</date><risdate>2000</risdate><volume>154</volume><issue>2</issue><spage>143</spage><epage>151</epage><pages>143-151</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>The total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from explants (cotyledons, petioles, hypocotyls and leaves) obtained from
Medicago arborea L. seedlings were evaluated. Total sugars were the major components in the calli and no significant differences between embryogenic and non-embryogenic calli were observed. In contrast, important differences between the embryogenic and non-embryogenic calli were observed for reducing sugars, the highest levels being observed in embryogenic calli. The highest starch levels were found in non-embryogenic calli developed in MS medium. During the development of somatic embryogenesis very low starch levels in the callus were found. During the first months of culture, no significant differences in the sucrose content were found between calli that produced embryos and those that did not. The most important differences in sucrose were seen between calli transferred to medium F0, which had the greatest embryogenic capacity, and those transferred to medium F6, which inhibited embryogenesis. In the latter case, an increase in sucrose was observed.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>10729613</pmid><doi>10.1016/S0168-9452(99)00251-4</doi><tpages>9</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biotechnology Embryogenic calli Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology In vitro embryo culture Medicago arborea Metabolism Methods. Procedures. Technologies Non-embryogenic calli Photosynthesis, respiration. Anabolism, catabolism Plant cells and fungal cells Plant physiology and development Reducing sugars Starch Sucrose Total sugars |
| title | Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L |
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