Production of TNF-alpha by primary cultures of human keratinocytes challenged with loxosceles gaucho venom
Primary cultures of human keratinocytes were challenged with increasing doses from 10 ng/mL to 2 g/mL of Loxosceles gaucho venom, responsible for dermonecrotic lesion in humans. TNF-alpha was investigated by bioassay and ELISA in the supernatant of the cultures challenged with 100 ng/mL, 500 ng/mL,...
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| Veröffentlicht in: | Revista do Instituto de Medicina Tropical de São Paulo 1999-05, Vol.41 (3), p.179-182 |
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| container_title | Revista do Instituto de Medicina Tropical de São Paulo |
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| creator | Malaque, CM Ori, M Santos, SA Andrade, DR |
| description | Primary cultures of human keratinocytes were challenged with increasing doses from 10 ng/mL to 2 g/mL of Loxosceles gaucho venom, responsible for dermonecrotic lesion in humans. TNF-alpha was investigated by bioassay and ELISA in the supernatant of the cultures challenged with 100 ng/mL, 500 ng/mL, 1 and 2 g/mL of venom. TNF-alpha was detected by bioassay in the supernatant of cultures challenged with 100 ng/mL, after 6 h. The cytokine was detected by ELISA in the supernatant of the cells challenged with doses of l g/mL, after 6 and 12 h. The results point out the capacity of this venom to activate the keratinocytes in primary cultures to produce TNF-alpha. The production of cytokines could contribute to the local inflammatory process in patients bitten by Loxosceles sp. |
| doi_str_mv | 10.1590/s0036-46651999000300009 |
| format | Article |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| title | Production of TNF-alpha by primary cultures of human keratinocytes challenged with loxosceles gaucho venom |
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