Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification

Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification

From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of structural biology 1997-12, Vol.120 (3), p.387-395
Hauptverfasser: Walz, Jochen, Typke, Dieter, Nitsch, Michael, Koster, Abraham J., Hegerl, Reiner, Baumeister, Wolfgang
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 395
container_issue 3
container_start_page 387
container_title Journal of structural biology
container_volume 120
creator Walz, Jochen
Typke, Dieter
Nitsch, Michael
Koster, Abraham J.
Hegerl, Reiner
Baumeister, Wolfgang
description From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level.
doi_str_mv 10.1006/jsbi.1997.3934
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859125898</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1047847797939344</els_id><sourcerecordid>1859125898</sourcerecordid><originalsourceid>FETCH-LOGICAL-c407t-6d74b884723e3ec7c10a9e20e2516e8ed1d473a0f20d3b2a62dc57c8d25c33633</originalsourceid><addsrcrecordid>eNp1kL1v2zAQxYmgQZqmXbMV4NhFLr8kkt0Mx20NJMgQZyYo8uTQoCSXlAP4vy8FG9063R3eu4e7H0L3lCwoIc33fW7DgmotF1xzcYVuKdF1pZpafph7ISslpPyIPuW8J4QIyugNutFCUC3oLQrrCG5K44C3Yz_ukj28nfDY4Zcw7CLgjYNq3bfgPXj8ZF0qprJwjJB_4O1bAqgeQg9DDuNgI17GsBvKOGE7eLyKNufQBWenIn9G152NGb5c6h16_bnern5Xj8-_NqvlY-UEkVPVeClaVW5mHDg46SixGhgBVtMGFHjqheSWdIx43jLbMO9q6ZRnteO84fwOfTvnHtL45wh5Mn3IDmK0A4zHbKiqNWW10qpYF2dr-SvnBJ05pNDbdDKUmJmumemama6Z6ZaFr5fsY9uD_2e_4Cy6OutQHnwPkEx2AQYHPqSC2fgx_C_6L9C1idw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1859125898</pqid></control><display><type>article</type><title>Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification</title><source>Elsevier ScienceDirect Journals</source><creator>Walz, Jochen ; Typke, Dieter ; Nitsch, Michael ; Koster, Abraham J. ; Hegerl, Reiner ; Baumeister, Wolfgang</creator><creatorcontrib>Walz, Jochen ; Typke, Dieter ; Nitsch, Michael ; Koster, Abraham J. ; Hegerl, Reiner ; Baumeister, Wolfgang</creatorcontrib><description>From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level.</description><identifier>ISSN: 1047-8477</identifier><identifier>EISSN: 1095-8657</identifier><identifier>DOI: 10.1006/jsbi.1997.3934</identifier><identifier>PMID: 9441941</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><ispartof>Journal of structural biology, 1997-12, Vol.120 (3), p.387-395</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-6d74b884723e3ec7c10a9e20e2516e8ed1d473a0f20d3b2a62dc57c8d25c33633</citedby><cites>FETCH-LOGICAL-c407t-6d74b884723e3ec7c10a9e20e2516e8ed1d473a0f20d3b2a62dc57c8d25c33633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1047847797939344$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9441941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walz, Jochen</creatorcontrib><creatorcontrib>Typke, Dieter</creatorcontrib><creatorcontrib>Nitsch, Michael</creatorcontrib><creatorcontrib>Koster, Abraham J.</creatorcontrib><creatorcontrib>Hegerl, Reiner</creatorcontrib><creatorcontrib>Baumeister, Wolfgang</creatorcontrib><title>Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification</title><title>Journal of structural biology</title><addtitle>J Struct Biol</addtitle><description>From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level.</description><issn>1047-8477</issn><issn>1095-8657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp1kL1v2zAQxYmgQZqmXbMV4NhFLr8kkt0Mx20NJMgQZyYo8uTQoCSXlAP4vy8FG9063R3eu4e7H0L3lCwoIc33fW7DgmotF1xzcYVuKdF1pZpafph7ISslpPyIPuW8J4QIyugNutFCUC3oLQrrCG5K44C3Yz_ukj28nfDY4Zcw7CLgjYNq3bfgPXj8ZF0qprJwjJB_4O1bAqgeQg9DDuNgI17GsBvKOGE7eLyKNufQBWenIn9G152NGb5c6h16_bnern5Xj8-_NqvlY-UEkVPVeClaVW5mHDg46SixGhgBVtMGFHjqheSWdIx43jLbMO9q6ZRnteO84fwOfTvnHtL45wh5Mn3IDmK0A4zHbKiqNWW10qpYF2dr-SvnBJ05pNDbdDKUmJmumemama6Z6ZaFr5fsY9uD_2e_4Cy6OutQHnwPkEx2AQYHPqSC2fgx_C_6L9C1idw</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Walz, Jochen</creator><creator>Typke, Dieter</creator><creator>Nitsch, Michael</creator><creator>Koster, Abraham J.</creator><creator>Hegerl, Reiner</creator><creator>Baumeister, Wolfgang</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification</title><author>Walz, Jochen ; Typke, Dieter ; Nitsch, Michael ; Koster, Abraham J. ; Hegerl, Reiner ; Baumeister, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-6d74b884723e3ec7c10a9e20e2516e8ed1d473a0f20d3b2a62dc57c8d25c33633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walz, Jochen</creatorcontrib><creatorcontrib>Typke, Dieter</creatorcontrib><creatorcontrib>Nitsch, Michael</creatorcontrib><creatorcontrib>Koster, Abraham J.</creatorcontrib><creatorcontrib>Hegerl, Reiner</creatorcontrib><creatorcontrib>Baumeister, Wolfgang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of structural biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walz, Jochen</au><au>Typke, Dieter</au><au>Nitsch, Michael</au><au>Koster, Abraham J.</au><au>Hegerl, Reiner</au><au>Baumeister, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification</atitle><jtitle>Journal of structural biology</jtitle><addtitle>J Struct Biol</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>120</volume><issue>3</issue><spage>387</spage><epage>395</epage><pages>387-395</pages><issn>1047-8477</issn><eissn>1095-8657</eissn><abstract>From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9441941</pmid><doi>10.1006/jsbi.1997.3934</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1047-8477
ispartof Journal of structural biology, 1997-12, Vol.120 (3), p.387-395
issn 1047-8477
1095-8657
language eng
recordid cdi_proquest_miscellaneous_1859125898
source Elsevier ScienceDirect Journals
title Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T23%3A24%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electron%20Tomography%20of%20Single%20Ice-Embedded%20Macromolecules:%20Three-Dimensional%20Alignment%20and%20Classification&rft.jtitle=Journal%20of%20structural%20biology&rft.au=Walz,%20Jochen&rft.date=1997-12-01&rft.volume=120&rft.issue=3&rft.spage=387&rft.epage=395&rft.pages=387-395&rft.issn=1047-8477&rft.eissn=1095-8657&rft_id=info:doi/10.1006/jsbi.1997.3934&rft_dat=%3Cproquest_cross%3E1859125898%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1859125898&rft_id=info:pmid/9441941&rft_els_id=S1047847797939344&rfr_iscdi=true