Dipeptide transport by crustacean hepatopancreatic brush-border membrane vesicles

Epithelial brush-border membrane vesicles (BBMVs) of lobster (Homarus americanus) hepatopancreas were formed by a Mg2+ precipitation technique. In these BBMVs, [14C]glycylsarcosine ([14C]Gly-Sar) uptake was stimulated by a transmembrane proton gradient. transmembrane K+ diffusion potential (inside negative) stimulated [14C]Gly-Sar uptake above that observed with short-circuited vesicles, while an inwardly directed Na+ gradient had no stimulatory effect on peptide uptake. [14C]Gly-Sar influx (over 10 s) occurred by a low-affinity, saturable, proton-gradient-dependent carrier system (Kt=5.90±0.13 mmol l-1, Jmax=4662±487 pmol mg-1 protein 10 s-1; mean ± s.e.m., N=3). This carrier exhibited a high-affinity proton binding site (KH=235±25 nmol l-1; pK=6.6) and an apparent 1H+:1Gly-Sar transport stoichiometry. Influx of 0.1 mmol l-1 [14C]Gly-Sar into lobster hepatopancreatic BBMVs was significantly (P