Characterization of nuclear polyhedrosis viruses from three subspecies of Lambdina fiscellaria
The eastern hemlock looper (EHL), Lambdina fiscellaria fiscellaria, the western hemlock looper (WHL), L. fiscellaria lugubrosa, and the western oak looper (WOL), L. fiscellaria somniaria, are closely related insects that are pests in Canadian forests. We have used restriction endonuclease (REN) and...
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Veröffentlicht in: | Journal of invertebrate pathology 1997-03, Vol.69 (2), p.125-134 |
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creator | Levin, D.B. (University of Victoria, Victoria, BC, Canada.) Laitinen, A.M Clarke, T Lucarotti, C.J Morin, B Otvos, I.S |
description | The eastern hemlock looper (EHL), Lambdina fiscellaria fiscellaria, the western hemlock looper (WHL), L. fiscellaria lugubrosa, and the western oak looper (WOL), L. fiscellaria somniaria, are closely related insects that are pests in Canadian forests. We have used restriction endonuclease (REN) and Southern blot analyses to characterize DNA of nuclear polyhedrosis viruses (NPVs) isolated from each of the three subspecies of L. fiscellaria. REN analysis of EHL-NPV, WHL-NPV, and WOL-NPV DNAs revealed that they have nonidentical REN-banding patterns. NPV DNA extracted from WOL larvae collected from Douglas fir trees had a different REN pattern than NPV DNA extracted from WOL larvae collected from Garry oak trees. Hybridization of radiolabeled EHL-NPV genomic DNA revealed that the EHL- and WHL-NPVs share considerable DNA sequence similarity, as radio-labeled EHL-NPV DNA hybridized strongly with all WHL-NPV DNA bands. The EHL-NPV polyhedrin gene hybridized strongly with WHL-NPV DNA, but the hybridization patterns of the two NPVs were different. Very weak hybridization was detected between the EHL-NPV polyhedrin gene and DNAs of the two WOL-NPVs. Nucleotide sequence analysis revealed that the polyhedrin genes of EHL-NPV and WHL-NPV differ in sequence by only eight nucleotide substitutions (five third position and three second position changes, resulting in four amino acid substitutions). Phylogenetic analysis using the polyhedrin genes of EHL-NPV and WHL-NPV indicate that these two viruses cluster with the Group II NPVs as described by Zanotto, Kessing, and Maruniak |
doi_str_mv | 10.1006/jipa.1996.4633 |
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(University of Victoria, Victoria, BC, Canada.) ; Laitinen, A.M ; Clarke, T ; Lucarotti, C.J ; Morin, B ; Otvos, I.S</creator><creatorcontrib>Levin, D.B. (University of Victoria, Victoria, BC, Canada.) ; Laitinen, A.M ; Clarke, T ; Lucarotti, C.J ; Morin, B ; Otvos, I.S</creatorcontrib><description>The eastern hemlock looper (EHL), Lambdina fiscellaria fiscellaria, the western hemlock looper (WHL), L. fiscellaria lugubrosa, and the western oak looper (WOL), L. fiscellaria somniaria, are closely related insects that are pests in Canadian forests. We have used restriction endonuclease (REN) and Southern blot analyses to characterize DNA of nuclear polyhedrosis viruses (NPVs) isolated from each of the three subspecies of L. fiscellaria. REN analysis of EHL-NPV, WHL-NPV, and WOL-NPV DNAs revealed that they have nonidentical REN-banding patterns. NPV DNA extracted from WOL larvae collected from Douglas fir trees had a different REN pattern than NPV DNA extracted from WOL larvae collected from Garry oak trees. Hybridization of radiolabeled EHL-NPV genomic DNA revealed that the EHL- and WHL-NPVs share considerable DNA sequence similarity, as radio-labeled EHL-NPV DNA hybridized strongly with all WHL-NPV DNA bands. The EHL-NPV polyhedrin gene hybridized strongly with WHL-NPV DNA, but the hybridization patterns of the two NPVs were different. Very weak hybridization was detected between the EHL-NPV polyhedrin gene and DNAs of the two WOL-NPVs. Nucleotide sequence analysis revealed that the polyhedrin genes of EHL-NPV and WHL-NPV differ in sequence by only eight nucleotide substitutions (five third position and three second position changes, resulting in four amino acid substitutions). Phylogenetic analysis using the polyhedrin genes of EHL-NPV and WHL-NPV indicate that these two viruses cluster with the Group II NPVs as described by Zanotto, Kessing, and Maruniak</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1006/jipa.1996.4633</identifier><identifier>PMID: 9056463</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Biological and medical sciences ; CLONACION ; CLONAGE ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; GEOMETRIDAE ; HIBRIDACION DE ADN ; HYBRIDATION D'ADN ; Insecta ; Invertebrates ; Lambdina fiscellaria lugubrosa ; nuclear polyhedrosis virus ; Pathology ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; VIRUS POLIEDROSIS NUCLEAR ; VIRUS POLYEDROSE NUCLEAIRE</subject><ispartof>Journal of invertebrate pathology, 1997-03, Vol.69 (2), p.125-134</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2610541$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9056463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levin, D.B. (University of Victoria, Victoria, BC, Canada.)</creatorcontrib><creatorcontrib>Laitinen, A.M</creatorcontrib><creatorcontrib>Clarke, T</creatorcontrib><creatorcontrib>Lucarotti, C.J</creatorcontrib><creatorcontrib>Morin, B</creatorcontrib><creatorcontrib>Otvos, I.S</creatorcontrib><title>Characterization of nuclear polyhedrosis viruses from three subspecies of Lambdina fiscellaria</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>The eastern hemlock looper (EHL), Lambdina fiscellaria fiscellaria, the western hemlock looper (WHL), L. fiscellaria lugubrosa, and the western oak looper (WOL), L. fiscellaria somniaria, are closely related insects that are pests in Canadian forests. We have used restriction endonuclease (REN) and Southern blot analyses to characterize DNA of nuclear polyhedrosis viruses (NPVs) isolated from each of the three subspecies of L. fiscellaria. REN analysis of EHL-NPV, WHL-NPV, and WOL-NPV DNAs revealed that they have nonidentical REN-banding patterns. NPV DNA extracted from WOL larvae collected from Douglas fir trees had a different REN pattern than NPV DNA extracted from WOL larvae collected from Garry oak trees. Hybridization of radiolabeled EHL-NPV genomic DNA revealed that the EHL- and WHL-NPVs share considerable DNA sequence similarity, as radio-labeled EHL-NPV DNA hybridized strongly with all WHL-NPV DNA bands. The EHL-NPV polyhedrin gene hybridized strongly with WHL-NPV DNA, but the hybridization patterns of the two NPVs were different. Very weak hybridization was detected between the EHL-NPV polyhedrin gene and DNAs of the two WOL-NPVs. Nucleotide sequence analysis revealed that the polyhedrin genes of EHL-NPV and WHL-NPV differ in sequence by only eight nucleotide substitutions (five third position and three second position changes, resulting in four amino acid substitutions). Phylogenetic analysis using the polyhedrin genes of EHL-NPV and WHL-NPV indicate that these two viruses cluster with the Group II NPVs as described by Zanotto, Kessing, and Maruniak</description><subject>Biological and medical sciences</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>GEOMETRIDAE</subject><subject>HIBRIDACION DE ADN</subject><subject>HYBRIDATION D'ADN</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Lambdina fiscellaria lugubrosa</subject><subject>nuclear polyhedrosis virus</subject><subject>Pathology</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>VIRUS POLIEDROSIS NUCLEAR</subject><subject>VIRUS POLYEDROSE NUCLEAIRE</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp90EtLxDAQAOAgiq6rVw-C0IOIl66TpE2Toyy-YMGDerVM08SN9GXSCvrrDVg8epph5pthGEJOKKwogLh6dwOuqFJilQnOd8iCghIpSMh3yQKAsZQBpQfkMIR3iFku1D7ZV5CL6Bfkdb1Fj3o03n3j6Pou6W3STbox6JOhb762pvZ9cCH5dH4KJiTW920ybr0xSZiqMBjtYjVObbCtatdhYl3QpmnQOzwiexabYI7nuCQvtzfP6_t083j3sL7epJbJYkwZRQGsQkZrVVXCMlVYVSldZLmQtWGWYo1VUSBkNhOodW65ZGCgQKtkLvmSXP7uHXz_MZkwlu18RGf6KZRU5ooymgFEevE_FcAplUWEZzOcqtbU5eBdi_6rnH8X--dzH4PGxnrstAt_jAkKeUYjO_1lFvsS33wkL0-q4IxLzn8AleiG_g</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>Levin, D.B. 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(University of Victoria, Victoria, BC, Canada.) ; Laitinen, A.M ; Clarke, T ; Lucarotti, C.J ; Morin, B ; Otvos, I.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f287t-21a602ba21d9bb6f297f9b9c74568de2f1adab77a04f46acc5f3820e07af98583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>GEOMETRIDAE</topic><topic>HIBRIDACION DE ADN</topic><topic>HYBRIDATION D'ADN</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Lambdina fiscellaria lugubrosa</topic><topic>nuclear polyhedrosis virus</topic><topic>Pathology</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>VIRUS POLIEDROSIS NUCLEAR</topic><topic>VIRUS POLYEDROSE NUCLEAIRE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Levin, D.B. (University of Victoria, Victoria, BC, Canada.)</creatorcontrib><creatorcontrib>Laitinen, A.M</creatorcontrib><creatorcontrib>Clarke, T</creatorcontrib><creatorcontrib>Lucarotti, C.J</creatorcontrib><creatorcontrib>Morin, B</creatorcontrib><creatorcontrib>Otvos, I.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Levin, D.B. (University of Victoria, Victoria, BC, Canada.)</au><au>Laitinen, A.M</au><au>Clarke, T</au><au>Lucarotti, C.J</au><au>Morin, B</au><au>Otvos, I.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of nuclear polyhedrosis viruses from three subspecies of Lambdina fiscellaria</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>69</volume><issue>2</issue><spage>125</spage><epage>134</epage><pages>125-134</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>The eastern hemlock looper (EHL), Lambdina fiscellaria fiscellaria, the western hemlock looper (WHL), L. fiscellaria lugubrosa, and the western oak looper (WOL), L. fiscellaria somniaria, are closely related insects that are pests in Canadian forests. We have used restriction endonuclease (REN) and Southern blot analyses to characterize DNA of nuclear polyhedrosis viruses (NPVs) isolated from each of the three subspecies of L. fiscellaria. REN analysis of EHL-NPV, WHL-NPV, and WOL-NPV DNAs revealed that they have nonidentical REN-banding patterns. NPV DNA extracted from WOL larvae collected from Douglas fir trees had a different REN pattern than NPV DNA extracted from WOL larvae collected from Garry oak trees. Hybridization of radiolabeled EHL-NPV genomic DNA revealed that the EHL- and WHL-NPVs share considerable DNA sequence similarity, as radio-labeled EHL-NPV DNA hybridized strongly with all WHL-NPV DNA bands. The EHL-NPV polyhedrin gene hybridized strongly with WHL-NPV DNA, but the hybridization patterns of the two NPVs were different. Very weak hybridization was detected between the EHL-NPV polyhedrin gene and DNAs of the two WOL-NPVs. Nucleotide sequence analysis revealed that the polyhedrin genes of EHL-NPV and WHL-NPV differ in sequence by only eight nucleotide substitutions (five third position and three second position changes, resulting in four amino acid substitutions). Phylogenetic analysis using the polyhedrin genes of EHL-NPV and WHL-NPV indicate that these two viruses cluster with the Group II NPVs as described by Zanotto, Kessing, and Maruniak</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>9056463</pmid><doi>10.1006/jipa.1996.4633</doi><tpages>10</tpages></addata></record> |
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subjects | Biological and medical sciences CLONACION CLONAGE COMPOSICION QUIMICA COMPOSITION CHIMIQUE Fundamental and applied biological sciences. Psychology GENE GENES GEOMETRIDAE HIBRIDACION DE ADN HYBRIDATION D'ADN Insecta Invertebrates Lambdina fiscellaria lugubrosa nuclear polyhedrosis virus Pathology SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS VIRUS POLIEDROSIS NUCLEAR VIRUS POLYEDROSE NUCLEAIRE |
title | Characterization of nuclear polyhedrosis viruses from three subspecies of Lambdina fiscellaria |
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