Characterization of the genetic system of the xylose-fermenting yeast Pichia stipitis
High mutant frequencies indicated that the wild-type strains of Pichia stipitis are haploid. Sporulation ability of these clones pointed to a homothallic life cycle. Mating was induced by cultivation under nutritionally poor conditions on malt extract medium. Conjugation was followed immediately by...
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| Veröffentlicht in: | Current microbiology 1996-10, Vol.33 (4), p.237-242 |
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| container_title | Current microbiology |
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| creator | Melake, T. (Rheinisch-Westfalische Technische Hochschule Aachen, Aachen, Germany.) Passoth, V Klinner, U |
| description | High mutant frequencies indicated that the wild-type strains of Pichia stipitis are haploid. Sporulation ability of these clones pointed to a homothallic life cycle. Mating was induced by cultivation under nutritionally poor conditions on malt extract medium. Conjugation was followed immediately by sporulation. However, hybrids could be rescued by transferring the nascent zygotes to complete medium before meiosis had started. Under rich nutritional conditions, hybrids were mitotically stable and did not sporulate. The segregation pattern of auxotrophic markers of diploid zygotes indicated regular meiosis, although asci contained preferentially spore dyads |
| doi_str_mv | 10.1007/s002849900106 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Current microbiology, 1996-10, Vol.33 (4), p.237-242 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | ACCOUPLEMENT Biological and medical sciences CICLO VITAL CLONE CLONES COPULA CYCLE DE DEVELOPPEMENT ESPORAS FUNGICAS ESPORULACION FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology GENETICA GENETIQUE Growth, nutrition, metabolism, transports, enzymes. Molecular biology HIBRIDACION HYBRIDATION MEIOSE MEIOSIS Microbiology MITOSE MITOSIS Mycology NOMBRE CHROMOSOMIQUE NUMERO DE CROMOSOMAS PICHIA STIPITIS SEGREGACION SEGREGATION SPORE FONGIQUE SPORULATION XILOSA XYLOSE |
| title | Characterization of the genetic system of the xylose-fermenting yeast Pichia stipitis |
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