Geographic Distribution of Cytoplasmic Polyhedrosis Virus Infection in Douglas-Fir Tussock Moth Larvae,Orgyia pseudotsugata,in British Columbia
We have examined the geographic distribution and prevalence of insect-specific viral infections in Douglas-fir tussock moth populations in British Columbia. Nucleic acids extracted from field collected egg masses from 10 different locations in British Columbia were subjected to agarose gel electroph...
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| Veröffentlicht in: | Journal of invertebrate pathology 1996-05, Vol.67 (3), p.229-235 |
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| container_title | Journal of invertebrate pathology |
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| creator | Laitinen, Ann Marie Otvos, Imre S. Levin, David B. |
| description | We have examined the geographic distribution and prevalence of insect-specific viral infections in Douglas-fir tussock moth populations in British Columbia. Nucleic acids extracted from field collected egg masses from 10 different locations in British Columbia were subjected to agarose gel electrophoresis and Southern blot analysis. Electrophoresis of nucleic acids after digestion with RNase A at high and low ionic strengths showed that the extra bands detected in agarose gels were double-stranded RNA (dsRNA). The dsRNA banding pattern was similar to the electropherotype of cytoplasmic polyhedrosis virus type 5. CPV infections were found in 27 of 170 DFTM larvae reared from field collected egg masses from four of 10 sites examined in three geographic regions of British Columbia. The CPV-infection rate for the DFTM larvae ranged from 10 to 86% depending on the location of the site. |
| doi_str_mv | 10.1006/jipa.1996.0038 |
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Nucleic acids extracted from field collected egg masses from 10 different locations in British Columbia were subjected to agarose gel electrophoresis and Southern blot analysis. Electrophoresis of nucleic acids after digestion with RNase A at high and low ionic strengths showed that the extra bands detected in agarose gels were double-stranded RNA (dsRNA). The dsRNA banding pattern was similar to the electropherotype of cytoplasmic polyhedrosis virus type 5. CPV infections were found in 27 of 170 DFTM larvae reared from field collected egg masses from four of 10 sites examined in three geographic regions of British Columbia. The CPV-infection rate for the DFTM larvae ranged from 10 to 86% depending on the location of the site.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1006/jipa.1996.0038</identifier><identifier>PMID: 8812604</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; COLOMBIE BRITANNIQUE ; COLUMBIA BRITANICA ; cytoplasmic polyhedrosis virus ; DISTRIBUCION GEOGRAFICA ; DISTRIBUTION GEOGRAPHIQUE ; Douglas-fir tussock moth ; ETIOLOGIA ; ETIOLOGIE ; Fundamental and applied biological sciences. Psychology ; geographic distribution ; Insecta ; Invertebrates ; LARVAS ; LARVE ; Lepidoptera ; Lymantriidae ; Nuclear polyhedrosis virus ; ORGYIA PSEUDOTSUGATA ; Pathology ; VIROSE ; VIROSIS ; VIRUS DE INVERTEBRADOS ; VIRUS DES INVERTEBRES ; VIRUS POLIEDROSIS NUCLEAR ; VIRUS POLYEDROSE NUCLEAIRE</subject><ispartof>Journal of invertebrate pathology, 1996-05, Vol.67 (3), p.229-235</ispartof><rights>1996 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-60304a1fe0a6859d22739391e9cb723220d0e8853740278d3a84e19bb795ba553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jipa.1996.0038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3100191$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8812604$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laitinen, Ann Marie</creatorcontrib><creatorcontrib>Otvos, Imre S.</creatorcontrib><creatorcontrib>Levin, David B.</creatorcontrib><title>Geographic Distribution of Cytoplasmic Polyhedrosis Virus Infection in Douglas-Fir Tussock Moth Larvae,Orgyia pseudotsugata,in British Columbia</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>We have examined the geographic distribution and prevalence of insect-specific viral infections in Douglas-fir tussock moth populations in British Columbia. Nucleic acids extracted from field collected egg masses from 10 different locations in British Columbia were subjected to agarose gel electrophoresis and Southern blot analysis. Electrophoresis of nucleic acids after digestion with RNase A at high and low ionic strengths showed that the extra bands detected in agarose gels were double-stranded RNA (dsRNA). The dsRNA banding pattern was similar to the electropherotype of cytoplasmic polyhedrosis virus type 5. CPV infections were found in 27 of 170 DFTM larvae reared from field collected egg masses from four of 10 sites examined in three geographic regions of British Columbia. The CPV-infection rate for the DFTM larvae ranged from 10 to 86% depending on the location of the site.</description><subject>Biological and medical sciences</subject><subject>COLOMBIE BRITANNIQUE</subject><subject>COLUMBIA BRITANICA</subject><subject>cytoplasmic polyhedrosis virus</subject><subject>DISTRIBUCION GEOGRAFICA</subject><subject>DISTRIBUTION GEOGRAPHIQUE</subject><subject>Douglas-fir tussock moth</subject><subject>ETIOLOGIA</subject><subject>ETIOLOGIE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>geographic distribution</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>LARVAS</subject><subject>LARVE</subject><subject>Lepidoptera</subject><subject>Lymantriidae</subject><subject>Nuclear polyhedrosis virus</subject><subject>ORGYIA PSEUDOTSUGATA</subject><subject>Pathology</subject><subject>VIROSE</subject><subject>VIROSIS</subject><subject>VIRUS DE INVERTEBRADOS</subject><subject>VIRUS DES INVERTEBRES</subject><subject>VIRUS POLIEDROSIS NUCLEAR</subject><subject>VIRUS POLYEDROSE NUCLEAIRE</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAURi0EKkNhywIJyQuEWDTDtfNnL2FKS6VBRaJla904TsYliVM7qTRPwSvjMKPuYGVL37mfrXsIec1gzQCKj3d2xDWTslgDpOIJWTGQRQIC8qdkBcB5woGx5-RFCHcQb3khT8iJEIwXkK3I70vjWo_jzmp6bsPkbTVP1g3UNXSzn9zYYehj9t11-52pvQs20J_Wz4FeDY3Rf1k70HM3txFNLqynN3MITv-i39y0o1v0D2jOrn27t0jHYObaTWFuccKzOPfZ28mGHd24bu4riy_Jswa7YF4dz1Nye_HlZvM12V5fXm0-bROdcZiSAlLIkDUGsBC5rDkvU5lKZqSuSp5yDjUYIfK0zICXok5RZIbJqiplXmGep6fkw6F39O5-NmFSvQ3adB0Oxs1BsdjKmMyFiOj7_6N5ASXIpXN9AHVcU_CmUaO3Pfq9YqAWWWqRpRZZapEVB94em-eqN_UjfrQT83fHHIPGrvE4aBsesTR2Mski9uaANegUtj4itz9kwVkKyxviEJq4zQdrvAramkGb2vqoT9XO_ut7fwBedLkx</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Laitinen, Ann Marie</creator><creator>Otvos, Imre S.</creator><creator>Levin, David B.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960501</creationdate><title>Geographic Distribution of Cytoplasmic Polyhedrosis Virus Infection in Douglas-Fir Tussock Moth Larvae,Orgyia pseudotsugata,in British Columbia</title><author>Laitinen, Ann Marie ; Otvos, Imre S. ; Levin, David B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-60304a1fe0a6859d22739391e9cb723220d0e8853740278d3a84e19bb795ba553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>COLOMBIE BRITANNIQUE</topic><topic>COLUMBIA BRITANICA</topic><topic>cytoplasmic polyhedrosis virus</topic><topic>DISTRIBUCION GEOGRAFICA</topic><topic>DISTRIBUTION GEOGRAPHIQUE</topic><topic>Douglas-fir tussock moth</topic><topic>ETIOLOGIA</topic><topic>ETIOLOGIE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>geographic distribution</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>LARVAS</topic><topic>LARVE</topic><topic>Lepidoptera</topic><topic>Lymantriidae</topic><topic>Nuclear polyhedrosis virus</topic><topic>ORGYIA PSEUDOTSUGATA</topic><topic>Pathology</topic><topic>VIROSE</topic><topic>VIROSIS</topic><topic>VIRUS DE INVERTEBRADOS</topic><topic>VIRUS DES INVERTEBRES</topic><topic>VIRUS POLIEDROSIS NUCLEAR</topic><topic>VIRUS POLYEDROSE NUCLEAIRE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laitinen, Ann Marie</creatorcontrib><creatorcontrib>Otvos, Imre S.</creatorcontrib><creatorcontrib>Levin, David B.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laitinen, Ann Marie</au><au>Otvos, Imre S.</au><au>Levin, David B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Geographic Distribution of Cytoplasmic Polyhedrosis Virus Infection in Douglas-Fir Tussock Moth Larvae,Orgyia pseudotsugata,in British Columbia</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>67</volume><issue>3</issue><spage>229</spage><epage>235</epage><pages>229-235</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>We have examined the geographic distribution and prevalence of insect-specific viral infections in Douglas-fir tussock moth populations in British Columbia. Nucleic acids extracted from field collected egg masses from 10 different locations in British Columbia were subjected to agarose gel electrophoresis and Southern blot analysis. Electrophoresis of nucleic acids after digestion with RNase A at high and low ionic strengths showed that the extra bands detected in agarose gels were double-stranded RNA (dsRNA). The dsRNA banding pattern was similar to the electropherotype of cytoplasmic polyhedrosis virus type 5. CPV infections were found in 27 of 170 DFTM larvae reared from field collected egg masses from four of 10 sites examined in three geographic regions of British Columbia. The CPV-infection rate for the DFTM larvae ranged from 10 to 86% depending on the location of the site.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8812604</pmid><doi>10.1006/jipa.1996.0038</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences COLOMBIE BRITANNIQUE COLUMBIA BRITANICA cytoplasmic polyhedrosis virus DISTRIBUCION GEOGRAFICA DISTRIBUTION GEOGRAPHIQUE Douglas-fir tussock moth ETIOLOGIA ETIOLOGIE Fundamental and applied biological sciences. Psychology geographic distribution Insecta Invertebrates LARVAS LARVE Lepidoptera Lymantriidae Nuclear polyhedrosis virus ORGYIA PSEUDOTSUGATA Pathology VIROSE VIROSIS VIRUS DE INVERTEBRADOS VIRUS DES INVERTEBRES VIRUS POLIEDROSIS NUCLEAR VIRUS POLYEDROSE NUCLEAIRE |
| title | Geographic Distribution of Cytoplasmic Polyhedrosis Virus Infection in Douglas-Fir Tussock Moth Larvae,Orgyia pseudotsugata,in British Columbia |
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