Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility
The effects of tributyltin (TBT) on the energy metabolism and motility of fish spermatozoa were investigated in vitro in African catfish and common carp. A significant ( P
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| Veröffentlicht in: | Comparative biochemistry and physiology. Toxicology & pharmacology 2002-03, Vol.131 (3), p.335-344 |
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| creator | Rurangwa, E. Biegniewska, A. Slominska, E. Skorkowski, E.F. Ollevier, F. |
| description | The effects of tributyltin (TBT) on the energy metabolism and motility of fish spermatozoa were investigated in vitro in African catfish and common carp. A significant (
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| doi_str_mv | 10.1016/S1532-0456(02)00019-4 |
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P<0.05) decrease of the duration and the intensity of motility was observed in catfish spermatozoa exposed to 0.27 μg/l TBT for 24 h. Exposure of catfish spermatozoa to 2.7–27 μg/l TBT caused an instant decrease in ATP content. In the presence of 27 μg/l TBT approximately 55% of the initial ATP concentration in catfish semen was lost after 60 min incubation while AMP concentrations increased and the total adenine nucleotide (TAN) pool remained unchanged. The reduction in sperm ATP levels could not be attributed to cell death since viability decreased only slightly over the period of exposure. In carp by contrast, none of the adenylates concentrations studied (ATP, ADP and AMP) were affected by TBT exposure at any experimental condition. However, carp sperm motility was significantly reduced by exposure to 2.7 μg/l TBT. Among the enzymes investigated only lactate dehydrogenase (LDH) in catfish sperm was significantly (
P<0.01) affected by 27 μg/l TBT treatment with a reduction in activity of approximately 75%. Compared with carp sperm before TBT exposure, that of catfish had lower adenylate contents and overall lower enzymatic activities; this explains its slower sperm velocity and shorter duration of movement as measured by computer assisted sperm analysis (CASA). The present in vitro study shows that catfish spermatozoa are more sensitive to TBT exposure (and probably to other toxicants) than those of carp.</description><identifier>ISSN: 1532-0456</identifier><identifier>EISSN: 1878-1659</identifier><identifier>DOI: 10.1016/S1532-0456(02)00019-4</identifier><identifier>PMID: 11912058</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenine Nucleotides - analysis ; Adenine Nucleotides - metabolism ; Adenosine Diphosphate - analysis ; Adenosine Diphosphate - metabolism ; Adenosine Monophosphate - analysis ; Adenosine Monophosphate - metabolism ; Adenosine Triphosphate - analysis ; Adenosine Triphosphate - metabolism ; Adenylate ; Animals ; Carp ; Carps ; Catfish ; Catfishes ; Cell Survival - drug effects ; Clarias gariepinus ; Computer assisted sperm analysis ; Cyprinus carpio ; Dose-Response Relationship, Drug ; Enzyme ; Enzyme Activation - drug effects ; L-Lactate Dehydrogenase - metabolism ; Male ; Motility ; Semen - chemistry ; Species Specificity ; Sperm ; Sperm Count ; Sperm Motility - drug effects ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Trialkyltin Compounds - toxicity ; Tributyltin</subject><ispartof>Comparative biochemistry and physiology. Toxicology & pharmacology, 2002-03, Vol.131 (3), p.335-344</ispartof><rights>2002 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-81dd90afaabcb5c59dfc701f17e2de17396844d5bd25cdab3172fe2bb4137b9d3</citedby><cites>FETCH-LOGICAL-c458t-81dd90afaabcb5c59dfc701f17e2de17396844d5bd25cdab3172fe2bb4137b9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1532045602000194$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11912058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rurangwa, E.</creatorcontrib><creatorcontrib>Biegniewska, A.</creatorcontrib><creatorcontrib>Slominska, E.</creatorcontrib><creatorcontrib>Skorkowski, E.F.</creatorcontrib><creatorcontrib>Ollevier, F.</creatorcontrib><title>Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility</title><title>Comparative biochemistry and physiology. Toxicology & pharmacology</title><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><description>The effects of tributyltin (TBT) on the energy metabolism and motility of fish spermatozoa were investigated in vitro in African catfish and common carp. A significant (
P<0.05) decrease of the duration and the intensity of motility was observed in catfish spermatozoa exposed to 0.27 μg/l TBT for 24 h. Exposure of catfish spermatozoa to 2.7–27 μg/l TBT caused an instant decrease in ATP content. In the presence of 27 μg/l TBT approximately 55% of the initial ATP concentration in catfish semen was lost after 60 min incubation while AMP concentrations increased and the total adenine nucleotide (TAN) pool remained unchanged. The reduction in sperm ATP levels could not be attributed to cell death since viability decreased only slightly over the period of exposure. In carp by contrast, none of the adenylates concentrations studied (ATP, ADP and AMP) were affected by TBT exposure at any experimental condition. However, carp sperm motility was significantly reduced by exposure to 2.7 μg/l TBT. Among the enzymes investigated only lactate dehydrogenase (LDH) in catfish sperm was significantly (
P<0.01) affected by 27 μg/l TBT treatment with a reduction in activity of approximately 75%. Compared with carp sperm before TBT exposure, that of catfish had lower adenylate contents and overall lower enzymatic activities; this explains its slower sperm velocity and shorter duration of movement as measured by computer assisted sperm analysis (CASA). The present in vitro study shows that catfish spermatozoa are more sensitive to TBT exposure (and probably to other toxicants) than those of carp.</description><subject>Adenine Nucleotides - analysis</subject><subject>Adenine Nucleotides - metabolism</subject><subject>Adenosine Diphosphate - analysis</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Monophosphate - analysis</subject><subject>Adenosine Monophosphate - metabolism</subject><subject>Adenosine Triphosphate - analysis</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Adenylate</subject><subject>Animals</subject><subject>Carp</subject><subject>Carps</subject><subject>Catfish</subject><subject>Catfishes</subject><subject>Cell Survival - drug effects</subject><subject>Clarias gariepinus</subject><subject>Computer assisted sperm analysis</subject><subject>Cyprinus carpio</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme</subject><subject>Enzyme Activation - drug effects</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Male</subject><subject>Motility</subject><subject>Semen - chemistry</subject><subject>Species Specificity</subject><subject>Sperm</subject><subject>Sperm Count</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Trialkyltin Compounds - toxicity</subject><subject>Tributyltin</subject><issn>1532-0456</issn><issn>1878-1659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAURSNERUvhE0AeIRik9UniJGZSoao8pEodAGPLjxNdo9i-2E5F-kl8Jb7NRQwZHQ_W3sf2qqpXQC-AQn_5FVjb1LRj_VvavKOUAq-7J9UZjMNYQ8_403L-i5xWz1P6USDWQf-sOgXg0FA2nlW_b6YJdSZhIjlateR1ztaT4Ik06NdZZiQ6-Iw-E-kNQf-wOiRSZ3tvs8X0mMQZQ8ok7TG690QSZYPeobNazkTu9zFIvSM5kJQXs5K8Q-JQ76S3yW0F4Vdhy4qIZtFotiaZw0OQxIVsZ5vXF9XJJOeEL4_zvPr-8ebb9ef69u7Tl-sPt7Xu2JjrEYzhVE5SKq2YZtxMeqAwwYCNQRha3o9dZ5gyDdNGqhaGZsJGqQ7aQXHTnldvtt5y758LpiycTRrnWXoMSxIwMk550xSQbaCOIaWIk9hH62RcBVBxkCQeJYmDAUHLPEgSXcm9Pi5YlEPzL3W0UoCrDcDyzHuLUSRt0ZePsbHIEibY_6z4Azi6pyI</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Rurangwa, E.</creator><creator>Biegniewska, A.</creator><creator>Slominska, E.</creator><creator>Skorkowski, E.F.</creator><creator>Ollevier, F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20020301</creationdate><title>Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility</title><author>Rurangwa, E. ; Biegniewska, A. ; Slominska, E. ; Skorkowski, E.F. ; Ollevier, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-81dd90afaabcb5c59dfc701f17e2de17396844d5bd25cdab3172fe2bb4137b9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenine Nucleotides - analysis</topic><topic>Adenine Nucleotides - metabolism</topic><topic>Adenosine Diphosphate - analysis</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Monophosphate - analysis</topic><topic>Adenosine Monophosphate - metabolism</topic><topic>Adenosine Triphosphate - analysis</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Adenylate</topic><topic>Animals</topic><topic>Carp</topic><topic>Carps</topic><topic>Catfish</topic><topic>Catfishes</topic><topic>Cell Survival - drug effects</topic><topic>Clarias gariepinus</topic><topic>Computer assisted sperm analysis</topic><topic>Cyprinus carpio</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme</topic><topic>Enzyme Activation - drug effects</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Male</topic><topic>Motility</topic><topic>Semen - chemistry</topic><topic>Species Specificity</topic><topic>Sperm</topic><topic>Sperm Count</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Trialkyltin Compounds - toxicity</topic><topic>Tributyltin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rurangwa, E.</creatorcontrib><creatorcontrib>Biegniewska, A.</creatorcontrib><creatorcontrib>Slominska, E.</creatorcontrib><creatorcontrib>Skorkowski, E.F.</creatorcontrib><creatorcontrib>Ollevier, F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rurangwa, E.</au><au>Biegniewska, A.</au><au>Slominska, E.</au><au>Skorkowski, E.F.</au><au>Ollevier, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility</atitle><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>131</volume><issue>3</issue><spage>335</spage><epage>344</epage><pages>335-344</pages><issn>1532-0456</issn><eissn>1878-1659</eissn><abstract>The effects of tributyltin (TBT) on the energy metabolism and motility of fish spermatozoa were investigated in vitro in African catfish and common carp. A significant (
P<0.05) decrease of the duration and the intensity of motility was observed in catfish spermatozoa exposed to 0.27 μg/l TBT for 24 h. Exposure of catfish spermatozoa to 2.7–27 μg/l TBT caused an instant decrease in ATP content. In the presence of 27 μg/l TBT approximately 55% of the initial ATP concentration in catfish semen was lost after 60 min incubation while AMP concentrations increased and the total adenine nucleotide (TAN) pool remained unchanged. The reduction in sperm ATP levels could not be attributed to cell death since viability decreased only slightly over the period of exposure. In carp by contrast, none of the adenylates concentrations studied (ATP, ADP and AMP) were affected by TBT exposure at any experimental condition. However, carp sperm motility was significantly reduced by exposure to 2.7 μg/l TBT. Among the enzymes investigated only lactate dehydrogenase (LDH) in catfish sperm was significantly (
P<0.01) affected by 27 μg/l TBT treatment with a reduction in activity of approximately 75%. Compared with carp sperm before TBT exposure, that of catfish had lower adenylate contents and overall lower enzymatic activities; this explains its slower sperm velocity and shorter duration of movement as measured by computer assisted sperm analysis (CASA). The present in vitro study shows that catfish spermatozoa are more sensitive to TBT exposure (and probably to other toxicants) than those of carp.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11912058</pmid><doi>10.1016/S1532-0456(02)00019-4</doi><tpages>10</tpages></addata></record> |
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| ispartof | Comparative biochemistry and physiology. Toxicology & pharmacology, 2002-03, Vol.131 (3), p.335-344 |
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| subjects | Adenine Nucleotides - analysis Adenine Nucleotides - metabolism Adenosine Diphosphate - analysis Adenosine Diphosphate - metabolism Adenosine Monophosphate - analysis Adenosine Monophosphate - metabolism Adenosine Triphosphate - analysis Adenosine Triphosphate - metabolism Adenylate Animals Carp Carps Catfish Catfishes Cell Survival - drug effects Clarias gariepinus Computer assisted sperm analysis Cyprinus carpio Dose-Response Relationship, Drug Enzyme Enzyme Activation - drug effects L-Lactate Dehydrogenase - metabolism Male Motility Semen - chemistry Species Specificity Sperm Sperm Count Sperm Motility - drug effects Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - metabolism Trialkyltin Compounds - toxicity Tributyltin |
| title | Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility |
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