Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave
Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2016-12, Vol.66 (12), p.5345-5351 |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Makk, Judit Tóth, Erika M Anda, Dóra Pál, Sára Schumann, Peter Kovács, Attila L Mádl-Szőnyi, Judit Márialigeti, Károly Borsodi, Andrea K |
| description | Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0-9.0, with an optimum at pH 7.0. The temperature range for growth was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1 %), Deinococcus aquaticus PB314T (93.3 %) and Deinococcus aerophilus 5516T-11T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C16 : 0 and C16 : 1ω7c, and the cell-wall peptidoglycan type was A3β l-Orn-Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was |
| doi_str_mv | 10.1099/ijsem.0.001519 |
| format | Article |
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The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0-9.0, with an optimum at pH 7.0. The temperature range for growth was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1 %), Deinococcus aquaticus PB314T (93.3 %) and Deinococcus aerophilus 5516T-11T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C16 : 0 and C16 : 1ω7c, and the cell-wall peptidoglycan type was A3β l-Orn-Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38T (=NCAIM B.02630T=DSM 101791T).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.001519</identifier><identifier>PMID: 27667170</identifier><language>eng</language><publisher>England</publisher><subject>Bacterial Typing Techniques ; Base Composition ; Biofilms ; Caves - microbiology ; Deinococcus - classification ; Deinococcus - isolation & purification ; Deinococcus - radiation effects ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Fatty Acids - chemistry ; Gamma Rays ; Glycolipids - chemistry ; Hungary ; Peptidoglycan - chemistry ; Phospholipids - chemistry ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Vitamin K 2 - analogs & derivatives ; Vitamin K 2 - chemistry</subject><ispartof>International journal of systematic and evolutionary microbiology, 2016-12, Vol.66 (12), p.5345-5351</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-2c6c1dfaaec3bbd98f93308ed940b99f421f233295309d17feb8acc9983e73f53</citedby><cites>FETCH-LOGICAL-c335t-2c6c1dfaaec3bbd98f93308ed940b99f421f233295309d17feb8acc9983e73f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3732,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27667170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Makk, Judit</creatorcontrib><creatorcontrib>Tóth, Erika M</creatorcontrib><creatorcontrib>Anda, Dóra</creatorcontrib><creatorcontrib>Pál, Sára</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Kovács, Attila L</creatorcontrib><creatorcontrib>Mádl-Szőnyi, Judit</creatorcontrib><creatorcontrib>Márialigeti, Károly</creatorcontrib><creatorcontrib>Borsodi, Andrea K</creatorcontrib><title>Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0-9.0, with an optimum at pH 7.0. The temperature range for growth was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1 %), Deinococcus aquaticus PB314T (93.3 %) and Deinococcus aerophilus 5516T-11T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C16 : 0 and C16 : 1ω7c, and the cell-wall peptidoglycan type was A3β l-Orn-Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38T (=NCAIM B.02630T=DSM 101791T).</description><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Biofilms</subject><subject>Caves - microbiology</subject><subject>Deinococcus - classification</subject><subject>Deinococcus - isolation & purification</subject><subject>Deinococcus - radiation effects</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Gamma Rays</subject><subject>Glycolipids - chemistry</subject><subject>Hungary</subject><subject>Peptidoglycan - chemistry</subject><subject>Phospholipids - chemistry</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Vitamin K 2 - analogs & derivatives</subject><subject>Vitamin K 2 - chemistry</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kD1PwzAQhi0EoqWwMiKPDCTYcb48ovIpVWKBOXKcM3UVx2kuqdR_j6HQ6U6n5311egi55izmTMp7u0FwMYsZ4xmXJ2TO0zyPMibS0-Oe5DNygbgJTJozdk5mSZHnBS_YnGwfwXZee60npPXUKOjQIsU-pp3fxXdUUQfo-7VtrQ5n0BaQWvStGqGhZvAuILX1xraOonJ9C9SbcFvvm8GPaxicakNwsN0X1WoHl-TMqBbh6m8uyOfz08fyNVq9v7wtH1aRFiIbo0TnmjdGKdCirhtZGikEK6GRKaulNGnCTSJEIjPBZMMLA3WptJayFFAIk4kFuT309oPfToBj5SxqaFvVgZ-w4mVWcpYzwQMaH1A9eMQBTBXedWrYV5xVP5qrX80Vqw6aQ-Dmr3uqHTRH_N-r-AasE3rE</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Makk, Judit</creator><creator>Tóth, Erika M</creator><creator>Anda, Dóra</creator><creator>Pál, Sára</creator><creator>Schumann, Peter</creator><creator>Kovács, Attila L</creator><creator>Mádl-Szőnyi, Judit</creator><creator>Márialigeti, Károly</creator><creator>Borsodi, Andrea K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave</title><author>Makk, Judit ; Tóth, Erika M ; Anda, Dóra ; Pál, Sára ; Schumann, Peter ; Kovács, Attila L ; Mádl-Szőnyi, Judit ; Márialigeti, Károly ; Borsodi, Andrea K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-2c6c1dfaaec3bbd98f93308ed940b99f421f233295309d17feb8acc9983e73f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Biofilms</topic><topic>Caves - microbiology</topic><topic>Deinococcus - classification</topic><topic>Deinococcus - isolation & purification</topic><topic>Deinococcus - radiation effects</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Gamma Rays</topic><topic>Glycolipids - chemistry</topic><topic>Hungary</topic><topic>Peptidoglycan - chemistry</topic><topic>Phospholipids - chemistry</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Vitamin K 2 - analogs & derivatives</topic><topic>Vitamin K 2 - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Makk, Judit</creatorcontrib><creatorcontrib>Tóth, Erika M</creatorcontrib><creatorcontrib>Anda, Dóra</creatorcontrib><creatorcontrib>Pál, Sára</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Kovács, Attila L</creatorcontrib><creatorcontrib>Mádl-Szőnyi, Judit</creatorcontrib><creatorcontrib>Márialigeti, Károly</creatorcontrib><creatorcontrib>Borsodi, Andrea K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Makk, Judit</au><au>Tóth, Erika M</au><au>Anda, Dóra</au><au>Pál, Sára</au><au>Schumann, Peter</au><au>Kovács, Attila L</au><au>Mádl-Szőnyi, Judit</au><au>Márialigeti, Károly</au><au>Borsodi, Andrea K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2016-12</date><risdate>2016</risdate><volume>66</volume><issue>12</issue><spage>5345</spage><epage>5351</epage><pages>5345-5351</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0-9.0, with an optimum at pH 7.0. The temperature range for growth was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1 %), Deinococcus aquaticus PB314T (93.3 %) and Deinococcus aerophilus 5516T-11T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C16 : 0 and C16 : 1ω7c, and the cell-wall peptidoglycan type was A3β l-Orn-Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38T (=NCAIM B.02630T=DSM 101791T).</abstract><cop>England</cop><pmid>27667170</pmid><doi>10.1099/ijsem.0.001519</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Typing Techniques Base Composition Biofilms Caves - microbiology Deinococcus - classification Deinococcus - isolation & purification Deinococcus - radiation effects DNA, Bacterial - genetics DNA, Ribosomal - genetics Fatty Acids - chemistry Gamma Rays Glycolipids - chemistry Hungary Peptidoglycan - chemistry Phospholipids - chemistry Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Vitamin K 2 - analogs & derivatives Vitamin K 2 - chemistry |
| title | Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave |
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