Stabilizing Mutations and Calcium-Dependent Stability of Subtilisin

Stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis and screening, yet the effects of most stabilizing mutations are not understood in structural and energetic detail. This paper seeks to explain the longstanding observation that stabili...

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Veröffentlicht in:	Biochemistry (Easton) 2001-09, Vol.40 (35), p.10640-10644
Hauptverfasser:	Alexander, Patrick A, Ruan, Biao, Strausberg, Susan L, Bryan, Philip N
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - enzymology Bacillus subtilis Calcium - metabolism Cloning, Molecular Enzyme Stability Models, Molecular Mutation Protein Conformation Subtilisin - chemistry Subtilisin - genetics Subtilisin - isolation & purification Subtilisin - metabolism
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