Ubiquitin recognition by the proteasome
The 26S proteasome is a 2.5-MDa complex responsible for the selective, ATP-dependent degradation of ubiquitylated proteins in eukaryotic cells. Substrates in hundreds cellular pathways are timely ubiquitylated and converged to the proteasome by direct recognition or by multiple shuttle factors. Enga...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 2017-02, Vol.161 (2), p.113-124 |
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| container_title | Journal of biochemistry (Tokyo) |
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| creator | Saeki, Yasushi |
| description | The 26S proteasome is a 2.5-MDa complex responsible for the selective, ATP-dependent degradation of ubiquitylated proteins in eukaryotic cells. Substrates in hundreds cellular pathways are timely ubiquitylated and converged to the proteasome by direct recognition or by multiple shuttle factors. Engagement of substrate protein triggers conformational changes of the proteasome, which drive substrate unfolding, deubiquitylation and translocation of substrates to proteolytic sites. Recent studies have challenged the previous paradigm that Lys48-linked tetraubiquitin is a minimal degradation signal: in addition, monoubiquitylation or multiple short ubiquitylations can serve as the targeting signal for proteasomal degradation. In this review, I highlight recent advances in our understanding of the proteasome structure, the ubiquitin topology in proteasome targeting, and the cellular factors that regulate proteasomal degradation. |
| doi_str_mv | 10.1093/jb/mvw091 |
| format | Article |
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Substrates in hundreds cellular pathways are timely ubiquitylated and converged to the proteasome by direct recognition or by multiple shuttle factors. Engagement of substrate protein triggers conformational changes of the proteasome, which drive substrate unfolding, deubiquitylation and translocation of substrates to proteolytic sites. Recent studies have challenged the previous paradigm that Lys48-linked tetraubiquitin is a minimal degradation signal: in addition, monoubiquitylation or multiple short ubiquitylations can serve as the targeting signal for proteasomal degradation. 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| fulltext | fulltext |
| identifier | ISSN: 0021-924X |
| ispartof | Journal of biochemistry (Tokyo), 2017-02, Vol.161 (2), p.113-124 |
| issn | 0021-924X 1756-2651 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1857372191 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphatases - metabolism Cell Cycle Proteins - metabolism Humans Lysine - metabolism Models, Biological Proteasome Endopeptidase Complex - metabolism Proteolysis Signal Transduction Ubiquitin - metabolism Ubiquitin-Protein Ligases - metabolism Ubiquitinated Proteins - metabolism Valosin Containing Protein |
| title | Ubiquitin recognition by the proteasome |
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