Analysis of Phosphatidic Acid Binding and Regulation of PIPKI In Vitro and in Intact Cells

Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is a lipid second messenger that regulates a wide array of essential cellular events, such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, adhesion, and motility. To control the spatiotemporal production of PI(4,5)P2, the ac...

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Veröffentlicht in:	Methods in enzymology 2017, Vol.583, p.359-374
Hauptverfasser:	Tay, L W R, Wang, Z, Du, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Cytoskeleton - drug effects Actin Cytoskeleton - metabolism Animals Cercopithecus aethiops Cloning, Molecular COS Cells Enzyme Assays Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Humans Liposomes - chemistry Liposomes - metabolism Phosphatidic Acids - metabolism Phosphatidic Acids - pharmacology Phosphatidylinositol 4,5-Diphosphate - metabolism Phospholipase D - genetics Phospholipase D - metabolism Phosphotransferases (Alcohol Group Acceptor) - genetics Phosphotransferases (Alcohol Group Acceptor) - metabolism Protein Binding Recombinant Proteins - genetics Recombinant Proteins - metabolism Signal Transduction
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