Single-Molecule Insight Into Target Recognition by CRISPR-Cas Complexes

Ribonucleoprotein (RNP) complexes from CRISPR-Cas systems have attracted enormous interest since they can be easily and flexibly reprogrammed to target any desired locus for genome engineering and gene regulation applications. Basis for the programmability is a short RNA (crRNA) inside these complex...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Methods in enzymology 2017, Vol.582, p.239-273
Hauptverfasser:	Rutkauskas, M, Krivoy, A, Szczelkun, M D, Rouillon, C, Seidel, R
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR-Associated Proteins - chemistry CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics DNA - chemistry DNA - genetics DNA Helicases - chemistry Nucleotide Motifs Protein Conformation Ribonucleoproteins - chemistry Ribonucleoproteins - isolation & purification RNA - chemistry Single Molecule Imaging - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!