Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation
Strontium (Sr) ions were added to calcium- and phosphate-containing porous titanium oxide layers formed by micro-arc oxidation (MAO) of titanium (Ti) substrates to improve their osseointegration. An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcifi...
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| Veröffentlicht in: | Dental Materials Journal 2016/07/29, Vol.35(4), pp.627-634 |
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| creator | SATO, Mizuki CHEN, Peng TSUTSUMI, Yusuke SHIOTA, Makoto HANAWA, Takao KASUGAI, Shohei |
| description | Strontium (Sr) ions were added to calcium- and phosphate-containing porous titanium oxide layers formed by micro-arc oxidation (MAO) of titanium (Ti) substrates to improve their osseointegration. An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcification. Similar surface microporous morphologies of the oxide layers were observed for all specimens produced by MAO, while the contents of the incorporated Sr ions increased with increasing Sr concentrations in MAO electrolytes. The calcium- and phosphate-containing porous layers promoted the cell alkaline phosphatase (ALP) activity, while cell calcification was promoted by the Sr addition. In particular, the ALP activity significantly increased after 10 days of culture, and larger areas of calcified deposits were observed for the specimens treated with MAO electrolytes containing 0.15 mol L−1 of Sr species. The effect of Sr addition on the calcification of the MAO-treated Ti oxide layers was established in this study. |
| doi_str_mv | 10.4012/dmj.2016-032 |
| format | Article |
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An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcification. Similar surface microporous morphologies of the oxide layers were observed for all specimens produced by MAO, while the contents of the incorporated Sr ions increased with increasing Sr concentrations in MAO electrolytes. The calcium- and phosphate-containing porous layers promoted the cell alkaline phosphatase (ALP) activity, while cell calcification was promoted by the Sr addition. In particular, the ALP activity significantly increased after 10 days of culture, and larger areas of calcified deposits were observed for the specimens treated with MAO electrolytes containing 0.15 mol L−1 of Sr species. The effect of Sr addition on the calcification of the MAO-treated Ti oxide layers was established in this study.</description><identifier>ISSN: 0287-4547</identifier><identifier>EISSN: 1881-1361</identifier><identifier>DOI: 10.4012/dmj.2016-032</identifier><identifier>PMID: 27477229</identifier><language>eng</language><publisher>Japan: The Japanese Society for Dental Materials and Devices</publisher><subject>Calcification ; Calcium ; Coated Materials, Biocompatible ; Dentistry ; Electrolytes ; Electrolytic cells ; Humans ; Ions ; MC3T3-E1 ; Micro-arc oxidation ; Osseointegration ; Osteoblasts ; Osteogenic differentiation and calcification ; Oxidation-Reduction ; Oxides ; Phosphates ; Strontium ; Surface Properties ; Titanium ; Titanium base alloys ; Titanium oxides</subject><ispartof>Dental Materials Journal, 2016/07/29, Vol.35(4), pp.627-634</ispartof><rights>2016 The Japanese Society for Dental Materials and Devices</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c793t-7751e419aa931a6c043fc7bf662313f51b0fb8b9e00260d9a78fe9f584cf40d43</citedby><cites>FETCH-LOGICAL-c793t-7751e419aa931a6c043fc7bf662313f51b0fb8b9e00260d9a78fe9f584cf40d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27477229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SATO, Mizuki</creatorcontrib><creatorcontrib>CHEN, Peng</creatorcontrib><creatorcontrib>TSUTSUMI, Yusuke</creatorcontrib><creatorcontrib>SHIOTA, Makoto</creatorcontrib><creatorcontrib>HANAWA, Takao</creatorcontrib><creatorcontrib>KASUGAI, Shohei</creatorcontrib><creatorcontrib>Tokyo Medical and Dental University</creatorcontrib><creatorcontrib>Oral Implantology and Regenerative Dental Medicine</creatorcontrib><creatorcontrib>Department of Metallic Biomaterials</creatorcontrib><creatorcontrib>Institute of Biomaterials and Bioengineering</creatorcontrib><title>Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation</title><title>Dental Materials Journal</title><addtitle>Dent. Mater. J.</addtitle><description>Strontium (Sr) ions were added to calcium- and phosphate-containing porous titanium oxide layers formed by micro-arc oxidation (MAO) of titanium (Ti) substrates to improve their osseointegration. An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcification. Similar surface microporous morphologies of the oxide layers were observed for all specimens produced by MAO, while the contents of the incorporated Sr ions increased with increasing Sr concentrations in MAO electrolytes. The calcium- and phosphate-containing porous layers promoted the cell alkaline phosphatase (ALP) activity, while cell calcification was promoted by the Sr addition. In particular, the ALP activity significantly increased after 10 days of culture, and larger areas of calcified deposits were observed for the specimens treated with MAO electrolytes containing 0.15 mol L−1 of Sr species. The effect of Sr addition on the calcification of the MAO-treated Ti oxide layers was established in this study.</description><subject>Calcification</subject><subject>Calcium</subject><subject>Coated Materials, Biocompatible</subject><subject>Dentistry</subject><subject>Electrolytes</subject><subject>Electrolytic cells</subject><subject>Humans</subject><subject>Ions</subject><subject>MC3T3-E1</subject><subject>Micro-arc oxidation</subject><subject>Osseointegration</subject><subject>Osteoblasts</subject><subject>Osteogenic differentiation and calcification</subject><subject>Oxidation-Reduction</subject><subject>Oxides</subject><subject>Phosphates</subject><subject>Strontium</subject><subject>Surface Properties</subject><subject>Titanium</subject><subject>Titanium base alloys</subject><subject>Titanium oxides</subject><issn>0287-4547</issn><issn>1881-1361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxSMEotvCjTPykQMp4z-JnSNUpUWqxAXOluPYXa8SO9iOxH4fPijOpt1zL2PJ8_N743lV9QHDNQNMvgzT4ZoAbmug5FW1w0LgGtMWv652QASvWcP4RXWZ0gGAda0Qb6sLwhnnhHS76t-ttUZnFCxKOQaf3TIhF3xCwSOtRu2s0yqXmxWZowkpm9CPKuWE9DLmJZphZecQw5K2J8tUI-UHNO9Dmvcqm1oXZeW8848ou6z86hL-usGgUR1NTMiGOBWh_ogmp2OoVdQn4GT9rnpj1ZjM-6fzqvr9_fbXzX398PPux83Xh1rzjuaa8wYbhjulOopVq4FRq3lv25ZQTG2De7C96DsDQFoYOsWFNZ1tBNOWwcDoVfVp051j-LOYlOXkkjbjqLwpn5NYNA3llBD-ArSAgHkLL0BBUFHodYDPG1pWkFI0Vs7RTSoeJQa5pi1L2nJNW5a0C_7xSXnpy_bO8HO8BbjbgNItMY7Bj84beQhL9GWRcjjCYPxZEmgDTK420BJeCmWEY2joqvRtUzqkrB7N2UrF7PRoTnPRRrK1PM93buq9itJ4-h93NNZk</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>SATO, Mizuki</creator><creator>CHEN, Peng</creator><creator>TSUTSUMI, Yusuke</creator><creator>SHIOTA, Makoto</creator><creator>HANAWA, Takao</creator><creator>KASUGAI, Shohei</creator><general>The Japanese Society for Dental Materials and Devices</general><general>Japanese Society for Dental Materials and Devices</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>2016</creationdate><title>Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation</title><author>SATO, Mizuki ; CHEN, Peng ; TSUTSUMI, Yusuke ; SHIOTA, Makoto ; HANAWA, Takao ; KASUGAI, Shohei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c793t-7751e419aa931a6c043fc7bf662313f51b0fb8b9e00260d9a78fe9f584cf40d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Calcification</topic><topic>Calcium</topic><topic>Coated Materials, Biocompatible</topic><topic>Dentistry</topic><topic>Electrolytes</topic><topic>Electrolytic cells</topic><topic>Humans</topic><topic>Ions</topic><topic>MC3T3-E1</topic><topic>Micro-arc oxidation</topic><topic>Osseointegration</topic><topic>Osteoblasts</topic><topic>Osteogenic differentiation and calcification</topic><topic>Oxidation-Reduction</topic><topic>Oxides</topic><topic>Phosphates</topic><topic>Strontium</topic><topic>Surface Properties</topic><topic>Titanium</topic><topic>Titanium base alloys</topic><topic>Titanium oxides</topic><toplevel>online_resources</toplevel><creatorcontrib>SATO, Mizuki</creatorcontrib><creatorcontrib>CHEN, Peng</creatorcontrib><creatorcontrib>TSUTSUMI, Yusuke</creatorcontrib><creatorcontrib>SHIOTA, Makoto</creatorcontrib><creatorcontrib>HANAWA, Takao</creatorcontrib><creatorcontrib>KASUGAI, Shohei</creatorcontrib><creatorcontrib>Tokyo Medical and Dental University</creatorcontrib><creatorcontrib>Oral Implantology and Regenerative Dental Medicine</creatorcontrib><creatorcontrib>Department of Metallic Biomaterials</creatorcontrib><creatorcontrib>Institute of Biomaterials and Bioengineering</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Dental Materials Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SATO, Mizuki</au><au>CHEN, Peng</au><au>TSUTSUMI, Yusuke</au><au>SHIOTA, Makoto</au><au>HANAWA, Takao</au><au>KASUGAI, Shohei</au><aucorp>Tokyo Medical and Dental University</aucorp><aucorp>Oral Implantology and Regenerative Dental Medicine</aucorp><aucorp>Department of Metallic Biomaterials</aucorp><aucorp>Institute of Biomaterials and Bioengineering</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation</atitle><jtitle>Dental Materials Journal</jtitle><addtitle>Dent. Mater. J.</addtitle><date>2016</date><risdate>2016</risdate><volume>35</volume><issue>4</issue><spage>627</spage><epage>634</epage><pages>627-634</pages><issn>0287-4547</issn><eissn>1881-1361</eissn><abstract>Strontium (Sr) ions were added to calcium- and phosphate-containing porous titanium oxide layers formed by micro-arc oxidation (MAO) of titanium (Ti) substrates to improve their osseointegration. An MC3T3-E1 preosteoblast was used to evaluate the effect of the incorporated Sr species on cell calcification. Similar surface microporous morphologies of the oxide layers were observed for all specimens produced by MAO, while the contents of the incorporated Sr ions increased with increasing Sr concentrations in MAO electrolytes. The calcium- and phosphate-containing porous layers promoted the cell alkaline phosphatase (ALP) activity, while cell calcification was promoted by the Sr addition. In particular, the ALP activity significantly increased after 10 days of culture, and larger areas of calcified deposits were observed for the specimens treated with MAO electrolytes containing 0.15 mol L−1 of Sr species. The effect of Sr addition on the calcification of the MAO-treated Ti oxide layers was established in this study.</abstract><cop>Japan</cop><pub>The Japanese Society for Dental Materials and Devices</pub><pmid>27477229</pmid><doi>10.4012/dmj.2016-032</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Calcification Calcium Coated Materials, Biocompatible Dentistry Electrolytes Electrolytic cells Humans Ions MC3T3-E1 Micro-arc oxidation Osseointegration Osteoblasts Osteogenic differentiation and calcification Oxidation-Reduction Oxides Phosphates Strontium Surface Properties Titanium Titanium base alloys Titanium oxides |
| title | Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation |
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