Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell column
Anthocyanins are water‐soluble pigments that are liable for colors ranging from red to blue of most fruits, vegetables, and flowers. A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode...
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| Veröffentlicht in: | Journal of separation science 2016-10, Vol.39 (20), p.3927-3935 |
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| container_title | Journal of separation science |
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| creator | Yıldırım, Sercan Kadıoğlu, Asım Sağlam, Aykut Yaşar, Ahmet Sellitepe, Hasan Erdinç |
| description | Anthocyanins are water‐soluble pigments that are liable for colors ranging from red to blue of most fruits, vegetables, and flowers. A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode array detection. A 10% formic acid and acetonitrile mixture was employed as mobile phase in the gradient elution mode. Mobile phase composition, column temperature, flow rate, injection volume, and column conditioning time were optimized by employing a stepwise strategy. Using a C18 core–shell column (100 × 4.6 mm, 2.7 μm), the separation of six analytes was accomplished in less than 9.5 min with a run‐to‐run analysis time of 19 min. The developed method was validated with respect to linearity (r > 0.9999), limit of detection, limit of quantification, intra‐/interday precision ( |
| doi_str_mv | 10.1002/jssc.201600661 |
| format | Article |
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A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode array detection. A 10% formic acid and acetonitrile mixture was employed as mobile phase in the gradient elution mode. Mobile phase composition, column temperature, flow rate, injection volume, and column conditioning time were optimized by employing a stepwise strategy. Using a C18 core–shell column (100 × 4.6 mm, 2.7 μm), the separation of six analytes was accomplished in less than 9.5 min with a run‐to‐run analysis time of 19 min. The developed method was validated with respect to linearity (r > 0.9999), limit of detection, limit of quantification, intra‐/interday precision (<2%), accuracy (98.6–104.4%), and specificity. 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Sep. Science</addtitle><description>Anthocyanins are water‐soluble pigments that are liable for colors ranging from red to blue of most fruits, vegetables, and flowers. A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode array detection. A 10% formic acid and acetonitrile mixture was employed as mobile phase in the gradient elution mode. Mobile phase composition, column temperature, flow rate, injection volume, and column conditioning time were optimized by employing a stepwise strategy. Using a C18 core–shell column (100 × 4.6 mm, 2.7 μm), the separation of six analytes was accomplished in less than 9.5 min with a run‐to‐run analysis time of 19 min. The developed method was validated with respect to linearity (r > 0.9999), limit of detection, limit of quantification, intra‐/interday precision (<2%), accuracy (98.6–104.4%), and specificity. Afterwards, the method was applied to the determination of anthocyanins present in 15 different samples including fruits, fruit juices, and fruit wines.</description><subject>Anthocyanins</subject><subject>Arrays</subject><subject>Chromatography</subject><subject>Core-shell columns</subject><subject>Fast separation</subject><subject>Food analysis</subject><subject>Fruit juices</subject><subject>Fruits</subject><subject>High performance liquid chromatography</subject><subject>Juices</subject><subject>Linearity</subject><subject>Separation</subject><subject>Wines</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkk1v1DAQhiMEoqVw5YgsceFAyji2Y-eIVt0CqkCoII6W40w2XhJ7aycq-4P4n3i1ZQ-ckCzNh5_X8mjeonhJ4ZICVO-2KdnLCmgNUNf0UXFOayrKhlH--JRDfVY8S2kLQKVq4GlxVkkhgEt2XvxemzSTDmeMk_NmdsGT0BPj5yHYvfHOp1x0pI-IZIejiZvgXec8yaePi5vT22Mk28VZzNURP3TuncdE2j0Z3GYodxj7ECfjLZLR3S2uI3aIYTJz2ESzG_ZkSc5viCE2RCzTgOOY03GZ_PPiSW_GhC8e4kXxfX31bfWhvPly_XH1_qa0nMm6lDaP1wPnzCpVMUqZBCV6BqqlCAoN61SHppGsMhKQ27ZVquG9pC0q2iG7KN4c393FcLdgmvXkks3_MB7DkjRVQrCaNyD_A2WCC1qzA_r6H3QblujzIJmqGqk4FyxTrx6opZ2w07voJhP3-u-uMsCPwL0bcX-6p6APTtAHJ-iTE_Sn29uVAFlnWXmUuTTjr5PMxJ-6lkwK_ePztV5zqL6uKtCS_QHMmLb-</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Yıldırım, Sercan</creator><creator>Kadıoğlu, Asım</creator><creator>Sağlam, Aykut</creator><creator>Yaşar, Ahmet</creator><creator>Sellitepe, Hasan Erdinç</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>NPM</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell column</title><author>Yıldırım, Sercan ; Kadıoğlu, Asım ; Sağlam, Aykut ; Yaşar, Ahmet ; Sellitepe, Hasan Erdinç</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4376-7c890f0443c88231137085f308b1e08ea3d8dea9732a70e4cbb8894f71be81de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anthocyanins</topic><topic>Arrays</topic><topic>Chromatography</topic><topic>Core-shell columns</topic><topic>Fast separation</topic><topic>Food analysis</topic><topic>Fruit juices</topic><topic>Fruits</topic><topic>High performance liquid chromatography</topic><topic>Juices</topic><topic>Linearity</topic><topic>Separation</topic><topic>Wines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yıldırım, Sercan</creatorcontrib><creatorcontrib>Kadıoğlu, Asım</creatorcontrib><creatorcontrib>Sağlam, Aykut</creatorcontrib><creatorcontrib>Yaşar, Ahmet</creatorcontrib><creatorcontrib>Sellitepe, Hasan Erdinç</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yıldırım, Sercan</au><au>Kadıoğlu, Asım</au><au>Sağlam, Aykut</au><au>Yaşar, Ahmet</au><au>Sellitepe, Hasan Erdinç</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell column</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2016-10</date><risdate>2016</risdate><volume>39</volume><issue>20</issue><spage>3927</spage><epage>3935</epage><pages>3927-3935</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>Anthocyanins are water‐soluble pigments that are liable for colors ranging from red to blue of most fruits, vegetables, and flowers. A novel and fast method was developed for the determination of five anthocyanins and free pelargonidin by high‐performance liquid chromatography coupled to photodiode array detection. A 10% formic acid and acetonitrile mixture was employed as mobile phase in the gradient elution mode. Mobile phase composition, column temperature, flow rate, injection volume, and column conditioning time were optimized by employing a stepwise strategy. Using a C18 core–shell column (100 × 4.6 mm, 2.7 μm), the separation of six analytes was accomplished in less than 9.5 min with a run‐to‐run analysis time of 19 min. 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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Anthocyanins Arrays Chromatography Core-shell columns Fast separation Food analysis Fruit juices Fruits High performance liquid chromatography Juices Linearity Separation Wines |
| title | Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell column |
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