Gene transcription repression in Clostridium beijerinckii using CRISPR-dCas9

ABSTRACT CRISPR‐Cas9 has been explored as a powerful tool for genome engineering for many organisms. Meanwhile, dCas9 which lacks endonuclease activity but can still bind to target loci has been engineered for efficient gene transcription repression. Clostridium beijerinckii, an industrially signifi...

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Veröffentlicht in:	Biotechnology and bioengineering 2016-12, Vol.113 (12), p.2739-2743
Hauptverfasser:	Wang, Yi, Zhang, Zhong-Tian, Seo, Seung-Oh, Lynn, Patrick, Lu, Ting, Jin, Yong-Su, Blaschek, Hans P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Amylase amylase activity Amylases - biosynthesis Amylases - genetics Bacteriology Bioengineering Biotechnology Clostridium Clostridium beijerinckii Clostridium beijerinckii - genetics Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Associated Proteins - genetics CRISPR-dCas9 CRISPRi Culture Down-Regulation - genetics Epigenetic Repression - genetics Fermentation Gene expression Gene Expression Regulation, Bacterial - genetics gene transcription repression Genes genome engineering Genomics Microbiology Microorganisms Ribonucleic acids Starches Streptococcus pyogenes synthetic biology Transcription, Genetic - genetics Transcriptional Activation - genetics
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