Quantitative determination by screening ELISA and HPLC-MS/MS of microcystins LR, LY, LA, YR, RR, LF, LW, and nodularin in the water of Occhito lake and crops
The occurrence of harmful cyanobacterial blooms in surface waters is often accompanied by the production of a variety of cyanotoxins, and these toxins are designed to target in humans specific organs on which they act. When introduced into the soil ecosystem by spray irrigation of crops, they may af...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2016-11, Vol.408 (27), p.7699-7708 |
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| creator | Trifirò, Gianluca Barbaro, Elena Gambaro, Andrea Vita, Valeria Clausi, Maria Teresa Franchino, Cinzia Palumbo, M. Paola Floridi, Francesca De Pace, Rita |
| description | The occurrence of harmful cyanobacterial blooms in surface waters is often accompanied by the production of a variety of cyanotoxins, and these toxins are designed to target in humans specific organs on which they act. When introduced into the soil ecosystem by spray irrigation of crops, they may affect the same molecular pathways in plants having identical or similar target organs, tissues, cells, or biomolecules. There are also several indications that terrestrial plants, including crops, can bioaccumulate cyanotoxins and present, therefore, potential health hazards for humans. During this project, for monitoring purposes, water samples were collected from lake Occhito, in which there was an algal bloom (
Planktothrix rubescens
) in 2009, and from three tanks which acted as hydraulic junctions. In addition, crop samples irrigated with water from the three tanks mentioned above were also picked. Finally, the characterization of principal cyanobacteria was performed, to determine the presence of cyanotoxins such as microcystins and validate a method of screening ELISA for the determination of microcystins in vegetable samples and a confirmatory method by HPLC-ESI-MS/MS.
Graphical abstract
Occhito lake (
left
), microcystin LR (
center
), Tomato field in Foggia (
right
); figures below: ELISA (
left
), HPLC-MS/MS (
right
) |
| doi_str_mv | 10.1007/s00216-016-9867-3 |
| format | Article |
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Planktothrix rubescens
) in 2009, and from three tanks which acted as hydraulic junctions. In addition, crop samples irrigated with water from the three tanks mentioned above were also picked. Finally, the characterization of principal cyanobacteria was performed, to determine the presence of cyanotoxins such as microcystins and validate a method of screening ELISA for the determination of microcystins in vegetable samples and a confirmatory method by HPLC-ESI-MS/MS.
Graphical abstract
Occhito lake (
left
), microcystin LR (
center
), Tomato field in Foggia (
right
); figures below: ELISA (
left
), HPLC-MS/MS (
right
)</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-016-9867-3</identifier><identifier>PMID: 27544518</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Algae ; Amino acids ; Analytical Chemistry ; Aquatic ecosystems ; Bacterial Toxins - analysis ; Biochemistry ; Blooms ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, High Pressure Liquid ; Crops ; Crops, Agricultural - chemistry ; Cyanobacteria ; Cyanobacteria - pathogenicity ; Cyanobacteria - physiology ; ELISA ; Environmental Monitoring ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Food Science ; Harmful Algal Bloom ; Health hazards ; High performance liquid chromatography ; Identification and classification ; Laboratory Medicine ; Lakes ; Lakes - chemistry ; Lakes - microbiology ; Liquid chromatography ; Mass spectrometry ; Methods ; Microcystins ; Microcystins - analysis ; Microcystis ; Monitoring/Environmental Analysis ; Observations ; Organs ; Peptides, Cyclic - analysis ; Planktothrix rubescens ; Plant-water relationships ; Research Paper ; Screening ; Sensors ; Spray irrigation ; Surface water ; Tandem Mass Spectrometry ; Tanks ; Toxicity ; Toxins ; Vegetables - chemistry ; Water analysis ; Water Pollutants, Chemical - analysis ; Water quality ; Water sampling ; Water tanks</subject><ispartof>Analytical and bioanalytical chemistry, 2016-11, Vol.408 (27), p.7699-7708</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-739e7212e5b02784589a6dc82059ddc9693053ea4a747edf15b5ab3bb41adfa63</citedby><cites>FETCH-LOGICAL-c584t-739e7212e5b02784589a6dc82059ddc9693053ea4a747edf15b5ab3bb41adfa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-016-9867-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-016-9867-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27544518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trifirò, Gianluca</creatorcontrib><creatorcontrib>Barbaro, Elena</creatorcontrib><creatorcontrib>Gambaro, Andrea</creatorcontrib><creatorcontrib>Vita, Valeria</creatorcontrib><creatorcontrib>Clausi, Maria Teresa</creatorcontrib><creatorcontrib>Franchino, Cinzia</creatorcontrib><creatorcontrib>Palumbo, M. Paola</creatorcontrib><creatorcontrib>Floridi, Francesca</creatorcontrib><creatorcontrib>De Pace, Rita</creatorcontrib><title>Quantitative determination by screening ELISA and HPLC-MS/MS of microcystins LR, LY, LA, YR, RR, LF, LW, and nodularin in the water of Occhito lake and crops</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The occurrence of harmful cyanobacterial blooms in surface waters is often accompanied by the production of a variety of cyanotoxins, and these toxins are designed to target in humans specific organs on which they act. When introduced into the soil ecosystem by spray irrigation of crops, they may affect the same molecular pathways in plants having identical or similar target organs, tissues, cells, or biomolecules. There are also several indications that terrestrial plants, including crops, can bioaccumulate cyanotoxins and present, therefore, potential health hazards for humans. During this project, for monitoring purposes, water samples were collected from lake Occhito, in which there was an algal bloom (
Planktothrix rubescens
) in 2009, and from three tanks which acted as hydraulic junctions. In addition, crop samples irrigated with water from the three tanks mentioned above were also picked. Finally, the characterization of principal cyanobacteria was performed, to determine the presence of cyanotoxins such as microcystins and validate a method of screening ELISA for the determination of microcystins in vegetable samples and a confirmatory method by HPLC-ESI-MS/MS.
Graphical abstract
Occhito lake (
left
), microcystin LR (
center
), Tomato field in Foggia (
right
); figures below: ELISA (
left
), HPLC-MS/MS (
right
)</description><subject>Algae</subject><subject>Amino acids</subject><subject>Analytical Chemistry</subject><subject>Aquatic ecosystems</subject><subject>Bacterial Toxins - analysis</subject><subject>Biochemistry</subject><subject>Blooms</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Crops</subject><subject>Crops, Agricultural - chemistry</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - pathogenicity</subject><subject>Cyanobacteria - physiology</subject><subject>ELISA</subject><subject>Environmental Monitoring</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Food Science</subject><subject>Harmful Algal Bloom</subject><subject>Health hazards</subject><subject>High performance liquid chromatography</subject><subject>Identification and classification</subject><subject>Laboratory Medicine</subject><subject>Lakes</subject><subject>Lakes - chemistry</subject><subject>Lakes - microbiology</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Microcystins</subject><subject>Microcystins - analysis</subject><subject>Microcystis</subject><subject>Monitoring/Environmental Analysis</subject><subject>Observations</subject><subject>Organs</subject><subject>Peptides, Cyclic - analysis</subject><subject>Planktothrix rubescens</subject><subject>Plant-water relationships</subject><subject>Research Paper</subject><subject>Screening</subject><subject>Sensors</subject><subject>Spray irrigation</subject><subject>Surface water</subject><subject>Tandem Mass Spectrometry</subject><subject>Tanks</subject><subject>Toxicity</subject><subject>Toxins</subject><subject>Vegetables - chemistry</subject><subject>Water analysis</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water quality</subject><subject>Water sampling</subject><subject>Water tanks</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkttu1DAQhiMEoqXwANwgS9xwsWl9iA-5XK1aWilVoQtCvYqcZLJ1yTpbOwHtw_CuTLptVSEhVcnYY_ubP7bzJ8l7Rg8ZpfooUsqZSilGbpROxYtknylmUq4kffmYZ3wveRPjDaVMGqZeJ3tcyyyTzOwnf76O1g9usIP7BaSBAcLaeRz1nlRbEusA4J1fkePibDkn1jfk9EuxSM-XR-dL0rdk7erQ19s4OB9JcTkjxRXGfEauML-cxicYP2Z3pb5vxs4G5wm-wzWQ3xY_OMlc1PW1G3rS2Z9wh6LqJr5NXrW2i_Duvj9Ivp8cf1ucpsXF57PFvEhrabIh1SIHzRkHWVGuTSZNblVTG05l3jR1rnJBpQCbWZ1paFomK2krUVUZs01rlThIPu10N6G_HSEO5drFGrrOeujHWDIjpZBZruUzUKEFV1Tx56BSGGw0oh__QW_6MXg8M1LcME5zOgke7qiV7aB0vu2HYGt8GsDf0HtoHc7PM00VGoBP52K7ArzNGAO05Sa4tQ3bktFyslC5s1CJFionC5UCaz7cb2Ws1tA8Vjx4BgG-AyIu-RWEJ3v9r-pfXR_MLg</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Trifirò, Gianluca</creator><creator>Barbaro, Elena</creator><creator>Gambaro, Andrea</creator><creator>Vita, Valeria</creator><creator>Clausi, Maria Teresa</creator><creator>Franchino, Cinzia</creator><creator>Palumbo, M. 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Paola ; Floridi, Francesca ; De Pace, Rita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-739e7212e5b02784589a6dc82059ddc9693053ea4a747edf15b5ab3bb41adfa63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Algae</topic><topic>Amino acids</topic><topic>Analytical Chemistry</topic><topic>Aquatic ecosystems</topic><topic>Bacterial Toxins - analysis</topic><topic>Biochemistry</topic><topic>Blooms</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Crops</topic><topic>Crops, Agricultural - chemistry</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - pathogenicity</topic><topic>Cyanobacteria - physiology</topic><topic>ELISA</topic><topic>Environmental Monitoring</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Food Science</topic><topic>Harmful Algal Bloom</topic><topic>Health hazards</topic><topic>High performance liquid chromatography</topic><topic>Identification and classification</topic><topic>Laboratory Medicine</topic><topic>Lakes</topic><topic>Lakes - chemistry</topic><topic>Lakes - microbiology</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Microcystins</topic><topic>Microcystins - analysis</topic><topic>Microcystis</topic><topic>Monitoring/Environmental Analysis</topic><topic>Observations</topic><topic>Organs</topic><topic>Peptides, Cyclic - analysis</topic><topic>Planktothrix rubescens</topic><topic>Plant-water relationships</topic><topic>Research Paper</topic><topic>Screening</topic><topic>Sensors</topic><topic>Spray irrigation</topic><topic>Surface water</topic><topic>Tandem Mass Spectrometry</topic><topic>Tanks</topic><topic>Toxicity</topic><topic>Toxins</topic><topic>Vegetables - chemistry</topic><topic>Water analysis</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water quality</topic><topic>Water sampling</topic><topic>Water tanks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trifirò, Gianluca</creatorcontrib><creatorcontrib>Barbaro, Elena</creatorcontrib><creatorcontrib>Gambaro, Andrea</creatorcontrib><creatorcontrib>Vita, Valeria</creatorcontrib><creatorcontrib>Clausi, Maria Teresa</creatorcontrib><creatorcontrib>Franchino, Cinzia</creatorcontrib><creatorcontrib>Palumbo, M. 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Paola</au><au>Floridi, Francesca</au><au>De Pace, Rita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative determination by screening ELISA and HPLC-MS/MS of microcystins LR, LY, LA, YR, RR, LF, LW, and nodularin in the water of Occhito lake and crops</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>408</volume><issue>27</issue><spage>7699</spage><epage>7708</epage><pages>7699-7708</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The occurrence of harmful cyanobacterial blooms in surface waters is often accompanied by the production of a variety of cyanotoxins, and these toxins are designed to target in humans specific organs on which they act. When introduced into the soil ecosystem by spray irrigation of crops, they may affect the same molecular pathways in plants having identical or similar target organs, tissues, cells, or biomolecules. There are also several indications that terrestrial plants, including crops, can bioaccumulate cyanotoxins and present, therefore, potential health hazards for humans. During this project, for monitoring purposes, water samples were collected from lake Occhito, in which there was an algal bloom (
Planktothrix rubescens
) in 2009, and from three tanks which acted as hydraulic junctions. In addition, crop samples irrigated with water from the three tanks mentioned above were also picked. Finally, the characterization of principal cyanobacteria was performed, to determine the presence of cyanotoxins such as microcystins and validate a method of screening ELISA for the determination of microcystins in vegetable samples and a confirmatory method by HPLC-ESI-MS/MS.
Graphical abstract
Occhito lake (
left
), microcystin LR (
center
), Tomato field in Foggia (
right
); figures below: ELISA (
left
), HPLC-MS/MS (
right
)</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27544518</pmid><doi>10.1007/s00216-016-9867-3</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2016-11, Vol.408 (27), p.7699-7708 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1855354975 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Algae Amino acids Analytical Chemistry Aquatic ecosystems Bacterial Toxins - analysis Biochemistry Blooms Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography Chromatography, High Pressure Liquid Crops Crops, Agricultural - chemistry Cyanobacteria Cyanobacteria - pathogenicity Cyanobacteria - physiology ELISA Environmental Monitoring Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Food Science Harmful Algal Bloom Health hazards High performance liquid chromatography Identification and classification Laboratory Medicine Lakes Lakes - chemistry Lakes - microbiology Liquid chromatography Mass spectrometry Methods Microcystins Microcystins - analysis Microcystis Monitoring/Environmental Analysis Observations Organs Peptides, Cyclic - analysis Planktothrix rubescens Plant-water relationships Research Paper Screening Sensors Spray irrigation Surface water Tandem Mass Spectrometry Tanks Toxicity Toxins Vegetables - chemistry Water analysis Water Pollutants, Chemical - analysis Water quality Water sampling Water tanks |
| title | Quantitative determination by screening ELISA and HPLC-MS/MS of microcystins LR, LY, LA, YR, RR, LF, LW, and nodularin in the water of Occhito lake and crops |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T02%3A30%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20determination%20by%20screening%20ELISA%20and%20HPLC-MS/MS%20of%20microcystins%20LR,%20LY,%20LA,%20YR,%20RR,%20LF,%20LW,%20and%20nodularin%20in%20the%20water%20of%20Occhito%20lake%20and%20crops&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Trifir%C3%B2,%20Gianluca&rft.date=2016-11-01&rft.volume=408&rft.issue=27&rft.spage=7699&rft.epage=7708&rft.pages=7699-7708&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-016-9867-3&rft_dat=%3Cgale_proqu%3EA470665026%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1828120902&rft_id=info:pmid/27544518&rft_galeid=A470665026&rfr_iscdi=true |