Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells
Although fluoride is known to reduce the incidence of caries, chronic excessive fluoride exposure can impair human health, even resulting in fluorosis. Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating t...
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| Veröffentlicht in: | Biological trace element research 2015-09, Vol.167 (1), p.11-17 |
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| creator | He, Hong Wang, Hongmei Jiao, Yuguo Ma, Congli Zhang, Han Zhou, Zhou |
| description | Although fluoride is known to reduce the incidence of caries, chronic excessive fluoride exposure can impair human health, even resulting in fluorosis. Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10–160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p |
| doi_str_mv | 10.1007/s12011-015-0271-1 |
| format | Article |
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Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10–160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p < 0.05). In addition, the genes mRNA expression, including ANKRD1, CRSP6, KLF2, SBNO2, ZNF649, FANCM, PDGFA, RNF152, CDK10, and CETN2 changed in a concentration-dependent manner and increased with fluoride exposure concentration. The results suggest that overexposure to fluoride (160–320 μM) can induce cytotoxicity and regulate relevant genes expression. Our findings provide novel insights into the mechanisms of action of fluoride-induced toxicity.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-015-0271-1</identifier><identifier>PMID: 25726004</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Apoptosis - drug effects ; Apoptosis - genetics ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Line, Tumor ; cell proliferation ; Cell Proliferation - drug effects ; Cell Proliferation - genetics ; Cell Survival - drug effects ; Cell Survival - genetics ; cell viability ; Cells ; cultured cells ; Cytotoxicity ; Dose-Response Relationship, Drug ; Fluorides ; fluorosis ; Gene expression ; gene expression regulation ; genes ; Humans ; Life Sciences ; mechanism of action ; messenger RNA ; Nutrition ; Oncology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sodium ; sodium fluoride ; Sodium Fluoride - pharmacology ; Transcriptome - drug effects ; Transcriptome - genetics</subject><ispartof>Biological trace element research, 2015-09, Vol.167 (1), p.11-17</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-dc9f8b5907d1c6d8920f9bf17af215c8ed660ff2f5ac86142c2b5b29a3eadf4d3</citedby><cites>FETCH-LOGICAL-c499t-dc9f8b5907d1c6d8920f9bf17af215c8ed660ff2f5ac86142c2b5b29a3eadf4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-015-0271-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-015-0271-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25726004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Hong</creatorcontrib><creatorcontrib>Wang, Hongmei</creatorcontrib><creatorcontrib>Jiao, Yuguo</creatorcontrib><creatorcontrib>Ma, Congli</creatorcontrib><creatorcontrib>Zhang, Han</creatorcontrib><creatorcontrib>Zhou, Zhou</creatorcontrib><title>Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Although fluoride is known to reduce the incidence of caries, chronic excessive fluoride exposure can impair human health, even resulting in fluorosis. Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10–160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p < 0.05). In addition, the genes mRNA expression, including ANKRD1, CRSP6, KLF2, SBNO2, ZNF649, FANCM, PDGFA, RNF152, CDK10, and CETN2 changed in a concentration-dependent manner and increased with fluoride exposure concentration. The results suggest that overexposure to fluoride (160–320 μM) can induce cytotoxicity and regulate relevant genes expression. Our findings provide novel insights into the mechanisms of action of fluoride-induced toxicity.</description><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Proliferation - genetics</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - genetics</subject><subject>cell viability</subject><subject>Cells</subject><subject>cultured cells</subject><subject>Cytotoxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fluorides</subject><subject>fluorosis</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>mechanism of action</subject><subject>messenger RNA</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium</subject><subject>sodium fluoride</subject><subject>Sodium Fluoride - pharmacology</subject><subject>Transcriptome - drug effects</subject><subject>Transcriptome - genetics</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9v1DAQxS0EokvhA3ABS1x6Ccw4dhwf0bL9IxVRUXq2nNheXCXxYicSfPt6lYIQB04jzfzem9E8Ql4jvEcA-SEjA8QKUFTAJFb4hGxQCFWBZPCUbACbuuKq5SfkRc73ACiZqp-TEyYkawD4hux33rt-ptHT22jDMtLzYYkpWEfjROfvjt6kOATvkplD6ZjJ0gs3OfopFGFy0xzMQHc_D8nlfATCRC-X0Uz0683nq5axhm7dMOSX5Jk3Q3avHuspuTvffdteVtdfLq62H6-rnis1V7ZXvu2EAmmxb2yrGHjVeZTGMxR962zTgPfMC9O3DXLWs050TJnaGeu5rU_J2ep7SPHH4vKsx5D7coGZXFyyxlYIkFwiFPTdP-h9XNJUrtPYqFZKIbgoFK5Un2LOyXl9SGE06ZdG0McU9JqCLinoYwoai-bNo_PSjc7-Ufx-ewHYCuQymvYu_bX6P65vV5E3UZt9Clnf3RaoOAIoXqv6AbZRmf4</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>He, Hong</creator><creator>Wang, Hongmei</creator><creator>Jiao, Yuguo</creator><creator>Ma, Congli</creator><creator>Zhang, Han</creator><creator>Zhou, Zhou</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150901</creationdate><title>Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells</title><author>He, Hong ; Wang, Hongmei ; Jiao, Yuguo ; Ma, Congli ; Zhang, Han ; Zhou, Zhou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-dc9f8b5907d1c6d8920f9bf17af215c8ed660ff2f5ac86142c2b5b29a3eadf4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Apoptosis - 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Now the underlying mechanisms of fluoride-induced toxicity are not fully understood. So, we conducted this study with the purpose of investigating the effect of sodium fluoride (NaF) in human RPMI8226 cells. In this experiment, human RPMI8226 cells were cultured with varied doses of fluoride (10, 20, 40, 80, 160, 320 μM). After 48 h exposure, the change of cell viability was examined by CCK-8 assay, and also the messenger RNA (mRNA) expression of relevant genes was assessed by QRT-PCR. Compared to the control group, fluoride exposure increased the human RPMI8226 cells viability at relatively lower levels (10–160 μM); however, when the concentration reached to 320 μM, the cell proliferation was significantly inhibited (p < 0.05). In addition, the genes mRNA expression, including ANKRD1, CRSP6, KLF2, SBNO2, ZNF649, FANCM, PDGFA, RNF152, CDK10, and CETN2 changed in a concentration-dependent manner and increased with fluoride exposure concentration. The results suggest that overexposure to fluoride (160–320 μM) can induce cytotoxicity and regulate relevant genes expression. Our findings provide novel insights into the mechanisms of action of fluoride-induced toxicity.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25726004</pmid><doi>10.1007/s12011-015-0271-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Apoptosis - drug effects Apoptosis - genetics Biochemistry Biomedical and Life Sciences Biotechnology Cell Line, Tumor cell proliferation Cell Proliferation - drug effects Cell Proliferation - genetics Cell Survival - drug effects Cell Survival - genetics cell viability Cells cultured cells Cytotoxicity Dose-Response Relationship, Drug Fluorides fluorosis Gene expression gene expression regulation genes Humans Life Sciences mechanism of action messenger RNA Nutrition Oncology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Sodium sodium fluoride Sodium Fluoride - pharmacology Transcriptome - drug effects Transcriptome - genetics |
| title | Effect of Sodium Fluoride on the Proliferation and Gene Differential Expression in Human RPMI8226 Cells |
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