Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library

Long non-coding RNAs are identified using a high-throughput paired-guide RNA genomic deletion screen. CRISPR–Cas9 screens have been widely adopted to analyze coding-gene functions, but high-throughput screening of non-coding elements using this method is more challenging because indels caused by a s...

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Veröffentlicht in:	Nature biotechnology 2016-12, Vol.34 (12), p.1279-1286
Hauptverfasser:	Zhu, Shiyou, Li, Wei, Liu, Jingze, Chen, Chen-Hao, Liao, Qi, Xu, Ping, Xu, Han, Xiao, Tengfei, Cao, Zhongzheng, Peng, Jingyu, Yuan, Pengfei, Brown, Myles, Liu, Xiaole Shirley, Wei, Wensheng
Format:	Artikel
Sprache:	eng
Schlagworte:	13 13/31 13/44 38/23 38/39 38/43 631/1647/1513/1967/3196 631/1647/2163 631/61/191/1908 Agriculture Bioinformatics Biomedical Engineering/Biotechnology Biomedicine Biotechnology Chromosome Mapping - methods Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA Gene Deletion Gene expression Genetic Testing - methods Genome, Human - genetics Genomes Genomic libraries Genomics High-Throughput Nucleotide Sequencing - methods Humans Life Sciences Mammals Methods Mutagenesis Ribonucleic acid RNA RNA sequencing RNA, Long Noncoding - genetics Sequence Analysis, RNA - methods
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