Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations
This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) test and other Nuclear Abnormalities (NAs) in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application thr...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 2017-02, Vol.136, p.84-91 |
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| creator | López González, E.C. Larriera, A. Siroski, P.A. Poletta, G.L. |
| description | This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) test and other Nuclear Abnormalities (NAs) in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application through the eggshell.
Embryos were exposed (during all incubation: 70 days approximately) to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN test and analysis of other NAs in erythrocytes, as markers of genotoxicity.
Results indicated a significant increase in the frequency of MN for PANZ1000, END 10, CYP 1 and CYP 100 (p |
| doi_str_mv | 10.1016/j.ecoenv.2016.10.035 |
| format | Article |
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Embryos were exposed (during all incubation: 70 days approximately) to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN test and analysis of other NAs in erythrocytes, as markers of genotoxicity.
Results indicated a significant increase in the frequency of MN for PANZ1000, END 10, CYP 1 and CYP 100 (p<0.05), and in the frequency of other NAs including Buds: END 100, 1000 and CYP 10 (p<0.05), eccentric nuclei: END 1, 10, 1000, CYP 10, 100, 1000 (p<0.01) and END 100 (p<0.05), notched nuclei: END 1, 10 (p<0.01) and END 1000, CYP 10, 100,1000 (p<0.05), and total nuclear abnormalities: END 1, 10, 100, 1000, CYP 10, 100 and 1000 (p<0.01), and the positive control (PC) (p<0.05), compared with the negative control. It was demonstrated a concentration dependent-effect in MN frequency only for PANZ (R2=0.98; p<0.01).
Our study demonstrated that commercial formulations of pesticides induced genotoxic effects on C. latirostris, and NAs are a good indicator of genotoxicity in this specie.
[Display omitted]
•Pesticide formulations induced nuclear abnormalities on Caiman embryos.•Nuclear abnormality is a biomarker of genotoxicity on Crocodilians.•Pesticides did not affect size of Caiman hatchlings.]]></description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2016.10.035</identifier><identifier>PMID: 27821305</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Administration, Topical ; Alligators and Crocodiles - metabolism ; Animals ; Caiman latirostris ; Cypermethrin ; DNA Damage ; Embryo exposure ; Endosulfan ; Endosulfan - toxicity ; Erythrocytes - drug effects ; Genotoxicity ; Glycine - analogs & derivatives ; Glycine - toxicity ; Glyphosate ; Herbicides - toxicity ; Insecticides - toxicity ; Micronucleus Tests ; Mutagens - toxicity ; Ovum ; Pyrethrins - toxicity ; Reptiles ; Teratogens - toxicity ; Water Pollutants, Chemical - toxicity</subject><ispartof>Ecotoxicology and environmental safety, 2017-02, Vol.136, p.84-91</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-26eda617cce6b97f4324aeffc782126f5ae08dc32c213c339cd86fc1cfcace583</citedby><cites>FETCH-LOGICAL-c478t-26eda617cce6b97f4324aeffc782126f5ae08dc32c213c339cd86fc1cfcace583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ecoenv.2016.10.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27821305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>López González, E.C.</creatorcontrib><creatorcontrib>Larriera, A.</creatorcontrib><creatorcontrib>Siroski, P.A.</creatorcontrib><creatorcontrib>Poletta, G.L.</creatorcontrib><title>Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description><![CDATA[This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) test and other Nuclear Abnormalities (NAs) in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application through the eggshell.
Embryos were exposed (during all incubation: 70 days approximately) to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN test and analysis of other NAs in erythrocytes, as markers of genotoxicity.
Results indicated a significant increase in the frequency of MN for PANZ1000, END 10, CYP 1 and CYP 100 (p<0.05), and in the frequency of other NAs including Buds: END 100, 1000 and CYP 10 (p<0.05), eccentric nuclei: END 1, 10, 1000, CYP 10, 100, 1000 (p<0.01) and END 100 (p<0.05), notched nuclei: END 1, 10 (p<0.01) and END 1000, CYP 10, 100,1000 (p<0.05), and total nuclear abnormalities: END 1, 10, 100, 1000, CYP 10, 100 and 1000 (p<0.01), and the positive control (PC) (p<0.05), compared with the negative control. It was demonstrated a concentration dependent-effect in MN frequency only for PANZ (R2=0.98; p<0.01).
Our study demonstrated that commercial formulations of pesticides induced genotoxic effects on C. latirostris, and NAs are a good indicator of genotoxicity in this specie.
[Display omitted]
•Pesticide formulations induced nuclear abnormalities on Caiman embryos.•Nuclear abnormality is a biomarker of genotoxicity on Crocodilians.•Pesticides did not affect size of Caiman hatchlings.]]></description><subject>Administration, Topical</subject><subject>Alligators and Crocodiles - metabolism</subject><subject>Animals</subject><subject>Caiman latirostris</subject><subject>Cypermethrin</subject><subject>DNA Damage</subject><subject>Embryo exposure</subject><subject>Endosulfan</subject><subject>Endosulfan - toxicity</subject><subject>Erythrocytes - drug effects</subject><subject>Genotoxicity</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - toxicity</subject><subject>Glyphosate</subject><subject>Herbicides - toxicity</subject><subject>Insecticides - toxicity</subject><subject>Micronucleus Tests</subject><subject>Mutagens - toxicity</subject><subject>Ovum</subject><subject>Pyrethrins - toxicity</subject><subject>Reptiles</subject><subject>Teratogens - toxicity</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQtRAVXQr_ACEfyyGLncRJ9oJEV3xJrXqBs-Udj9lZJfZiOxX9N_zUOt3CkdN4nt7Mm-fH2Bsp1lLI7v1hjRDQ363r0hVoLRr1jK2k2IiqbmX7nK2EbPuqU7I5Zy9TOgghGqHUC3Ze90Mty3vF_twQxOBnGJG48ZaHvMfIHwETudn5ECczUiZMPHi-NTQZz0eTKYaUIyV-eRWDsVXyYc5oOTwy3vG9ybAfyf9M3LhcduK0i_fBE3D8fQxpjshz4Jacw4g-8yOmTEAWuSua8yIRfHrFzpwZE75-qhfsx-dP37dfq-vbL9-2H68raPshV3WH1nSyB8But-ld29StQedgcVp3ThkUg4WmhmIcmmYDdugcSHBgANXQXLDL095jDL_mcoqeKAGOo_EY5qTloJToZbdRhdqeqOXnUoro9DEWz_FeS6GXbPRBn7LRSzYLWrIpY2-fFObdhPbf0N8wCuHDiYDF5x1h1AkIPaCliJC1DfR_hQf1h6d-</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>López González, E.C.</creator><creator>Larriera, A.</creator><creator>Siroski, P.A.</creator><creator>Poletta, G.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>20170201</creationdate><title>Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations</title><author>López González, E.C. ; Larriera, A. ; Siroski, P.A. ; Poletta, G.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-26eda617cce6b97f4324aeffc782126f5ae08dc32c213c339cd86fc1cfcace583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Administration, Topical</topic><topic>Alligators and Crocodiles - metabolism</topic><topic>Animals</topic><topic>Caiman latirostris</topic><topic>Cypermethrin</topic><topic>DNA Damage</topic><topic>Embryo exposure</topic><topic>Endosulfan</topic><topic>Endosulfan - toxicity</topic><topic>Erythrocytes - drug effects</topic><topic>Genotoxicity</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - toxicity</topic><topic>Glyphosate</topic><topic>Herbicides - toxicity</topic><topic>Insecticides - toxicity</topic><topic>Micronucleus Tests</topic><topic>Mutagens - toxicity</topic><topic>Ovum</topic><topic>Pyrethrins - toxicity</topic><topic>Reptiles</topic><topic>Teratogens - toxicity</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López González, E.C.</creatorcontrib><creatorcontrib>Larriera, A.</creatorcontrib><creatorcontrib>Siroski, P.A.</creatorcontrib><creatorcontrib>Poletta, G.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López González, E.C.</au><au>Larriera, A.</au><au>Siroski, P.A.</au><au>Poletta, G.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>136</volume><spage>84</spage><epage>91</epage><pages>84-91</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><abstract><![CDATA[This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) test and other Nuclear Abnormalities (NAs) in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application through the eggshell.
Embryos were exposed (during all incubation: 70 days approximately) to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN test and analysis of other NAs in erythrocytes, as markers of genotoxicity.
Results indicated a significant increase in the frequency of MN for PANZ1000, END 10, CYP 1 and CYP 100 (p<0.05), and in the frequency of other NAs including Buds: END 100, 1000 and CYP 10 (p<0.05), eccentric nuclei: END 1, 10, 1000, CYP 10, 100, 1000 (p<0.01) and END 100 (p<0.05), notched nuclei: END 1, 10 (p<0.01) and END 1000, CYP 10, 100,1000 (p<0.05), and total nuclear abnormalities: END 1, 10, 100, 1000, CYP 10, 100 and 1000 (p<0.01), and the positive control (PC) (p<0.05), compared with the negative control. It was demonstrated a concentration dependent-effect in MN frequency only for PANZ (R2=0.98; p<0.01).
Our study demonstrated that commercial formulations of pesticides induced genotoxic effects on C. latirostris, and NAs are a good indicator of genotoxicity in this specie.
[Display omitted]
•Pesticide formulations induced nuclear abnormalities on Caiman embryos.•Nuclear abnormality is a biomarker of genotoxicity on Crocodilians.•Pesticides did not affect size of Caiman hatchlings.]]></abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>27821305</pmid><doi>10.1016/j.ecoenv.2016.10.035</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Ecotoxicology and environmental safety, 2017-02, Vol.136, p.84-91 |
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| subjects | Administration, Topical Alligators and Crocodiles - metabolism Animals Caiman latirostris Cypermethrin DNA Damage Embryo exposure Endosulfan Endosulfan - toxicity Erythrocytes - drug effects Genotoxicity Glycine - analogs & derivatives Glycine - toxicity Glyphosate Herbicides - toxicity Insecticides - toxicity Micronucleus Tests Mutagens - toxicity Ovum Pyrethrins - toxicity Reptiles Teratogens - toxicity Water Pollutants, Chemical - toxicity |
| title | Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations |
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