FGF11 induced by hypoxia interacts with HIF‐1α and enhances its stability
Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia‐inducible factor‐1α...
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| Veröffentlicht in: | FEBS letters 2017-01, Vol.591 (2), p.348-357 |
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| creator | Lee, Kyeong Won Yim, Hyung‐Soon Shin, Jihye Lee, Cheolju Lee, Jung‐Hyun Jeong, Jae‐Yeon |
| description | Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia‐inducible factor‐1α (HIF‐1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF‐1α protein, while FGF11 overexpression increased it, without affecting HIF‐1α mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF‐1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross‐regulation between HIF‐1α and FGF11, through which hypoxia‐induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF‐1α. |
| doi_str_mv | 10.1002/1873-3468.12547 |
| format | Article |
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Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia‐inducible factor‐1α (HIF‐1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF‐1α protein, while FGF11 overexpression increased it, without affecting HIF‐1α mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF‐1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross‐regulation between HIF‐1α and FGF11, through which hypoxia‐induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF‐1α.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1002/1873-3468.12547</identifier><identifier>PMID: 28027390</identifier><language>eng</language><publisher>England</publisher><subject>Cell Hypoxia ; Cell Line, Tumor ; FGF11 ; Fibroblast Growth Factors - metabolism ; Gene Expression Regulation ; Gene Knockdown Techniques ; HIF‐1α ; Humans ; hypoxia ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; intracellular fibroblast growth factor ; Protein Binding ; Protein Stability ; Proteolysis ; Transcription, Genetic</subject><ispartof>FEBS letters, 2017-01, Vol.591 (2), p.348-357</ispartof><rights>2016 Federation of European Biochemical Societies</rights><rights>2016 Federation of European Biochemical Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1873-3468.12547$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1873-3468.12547$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27928,27929,45578,45579,46413,46837</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28027390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Kyeong Won</creatorcontrib><creatorcontrib>Yim, Hyung‐Soon</creatorcontrib><creatorcontrib>Shin, Jihye</creatorcontrib><creatorcontrib>Lee, Cheolju</creatorcontrib><creatorcontrib>Lee, Jung‐Hyun</creatorcontrib><creatorcontrib>Jeong, Jae‐Yeon</creatorcontrib><title>FGF11 induced by hypoxia interacts with HIF‐1α and enhances its stability</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia‐inducible factor‐1α (HIF‐1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF‐1α protein, while FGF11 overexpression increased it, without affecting HIF‐1α mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF‐1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross‐regulation between HIF‐1α and FGF11, through which hypoxia‐induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF‐1α.</description><subject>Cell Hypoxia</subject><subject>Cell Line, Tumor</subject><subject>FGF11</subject><subject>Fibroblast Growth Factors - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Gene Knockdown Techniques</subject><subject>HIF‐1α</subject><subject>Humans</subject><subject>hypoxia</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>intracellular fibroblast growth factor</subject><subject>Protein Binding</subject><subject>Protein Stability</subject><subject>Proteolysis</subject><subject>Transcription, Genetic</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kL1OwzAUhS0EoqUwsyGPLCn-S2yPUDVtpUosMFu246pGaRriRCUbj8Cr8CI8BE9C0pYu9-ecc-_wAXCL0RgjRB6w4DSiLBFjTGLGz8DwpJyDIUKYRTGXdACuQnhD3S6wvAQDIhDhVKIhWKazFGPoi6yxLoOmheu23H543Um1q7StA9z5eg3ni_T38wv_fENdZNAVa11YF6Dv_FBr43Nft9fgYqXz4G6OfQRe0-nLZB4tn2eLyeMyKomUPJI2tiZhxCBmMLU8dsIQgk2WZaIrHDtHJUu4xlpbJFnM-SphiGinrY4TR0fg_vC3rLbvjQu12vhgXZ7rwm2boLCIKe8OhOyid8doYzYuU2XlN7pq1T-BLpAcAjufu_bkY6R6wKrHqXqcag9YpdMnsp_oH9mfbVc</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Lee, Kyeong Won</creator><creator>Yim, Hyung‐Soon</creator><creator>Shin, Jihye</creator><creator>Lee, Cheolju</creator><creator>Lee, Jung‐Hyun</creator><creator>Jeong, Jae‐Yeon</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>FGF11 induced by hypoxia interacts with HIF‐1α and enhances its stability</title><author>Lee, Kyeong Won ; Yim, Hyung‐Soon ; Shin, Jihye ; Lee, Cheolju ; Lee, Jung‐Hyun ; Jeong, Jae‐Yeon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2997-9c5cb642b04b13c75e8b221bddd8bdd71ee39467a1aac094577f6402aeaca56e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Cell Hypoxia</topic><topic>Cell Line, Tumor</topic><topic>FGF11</topic><topic>Fibroblast Growth Factors - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Gene Knockdown Techniques</topic><topic>HIF‐1α</topic><topic>Humans</topic><topic>hypoxia</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>intracellular fibroblast growth factor</topic><topic>Protein Binding</topic><topic>Protein Stability</topic><topic>Proteolysis</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Kyeong Won</creatorcontrib><creatorcontrib>Yim, Hyung‐Soon</creatorcontrib><creatorcontrib>Shin, Jihye</creatorcontrib><creatorcontrib>Lee, Cheolju</creatorcontrib><creatorcontrib>Lee, Jung‐Hyun</creatorcontrib><creatorcontrib>Jeong, Jae‐Yeon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Kyeong Won</au><au>Yim, Hyung‐Soon</au><au>Shin, Jihye</au><au>Lee, Cheolju</au><au>Lee, Jung‐Hyun</au><au>Jeong, Jae‐Yeon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FGF11 induced by hypoxia interacts with HIF‐1α and enhances its stability</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2017-01</date><risdate>2017</risdate><volume>591</volume><issue>2</issue><spage>348</spage><epage>357</epage><pages>348-357</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia‐inducible factor‐1α (HIF‐1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF‐1α protein, while FGF11 overexpression increased it, without affecting HIF‐1α mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF‐1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross‐regulation between HIF‐1α and FGF11, through which hypoxia‐induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF‐1α.</abstract><cop>England</cop><pmid>28027390</pmid><doi>10.1002/1873-3468.12547</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Cell Hypoxia Cell Line, Tumor FGF11 Fibroblast Growth Factors - metabolism Gene Expression Regulation Gene Knockdown Techniques HIF‐1α Humans hypoxia Hypoxia-Inducible Factor 1, alpha Subunit - metabolism intracellular fibroblast growth factor Protein Binding Protein Stability Proteolysis Transcription, Genetic |
| title | FGF11 induced by hypoxia interacts with HIF‐1α and enhances its stability |
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