Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice
Peptidoglycan monomer (PGM) originating from Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the inf...
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| Veröffentlicht in: | Vaccine 2002-10, Vol.20 (29), p.3543-3550 |
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| container_title | Vaccine |
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| creator | Halassy Špoljar, Beata Čimbora, Tamara Hanzl-Dujmović, Ivana Dojnović, Biserka Sabioncello, Ante Krstanović, Marina Tomašić, Jelka |
| description | Peptidoglycan monomer (PGM) originating from
Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T cell activation and cytokine networks in response to OVA. OVA-specific proliferative response as well as interferon-γ (IFN-γ) and interleukin-4 (IL-4) secretion in lymph node cell cultures of immunised mice were studied. Due to pharmacokinetic properties of PGM, namely its fast metabolism and excretion, special emphasis was on choosing the appropriate time for lymph node removal and duration of cell cultivation for each cytokine. PGM treatment in addition to OVA resulted in an increase of lymph node cellularity, stimulation of OVA-specific IFN-γ and IL-4 production as well as of OVA-specific proliferative response. Results demonstrate that PGM stimulated both Th1 and Th2 subpopulations. |
| doi_str_mv | 10.1016/S0264-410X(02)00336-5 |
| format | Article |
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Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T cell activation and cytokine networks in response to OVA. OVA-specific proliferative response as well as interferon-γ (IFN-γ) and interleukin-4 (IL-4) secretion in lymph node cell cultures of immunised mice were studied. Due to pharmacokinetic properties of PGM, namely its fast metabolism and excretion, special emphasis was on choosing the appropriate time for lymph node removal and duration of cell cultivation for each cytokine. PGM treatment in addition to OVA resulted in an increase of lymph node cellularity, stimulation of OVA-specific IFN-γ and IL-4 production as well as of OVA-specific proliferative response. Results demonstrate that PGM stimulated both Th1 and Th2 subpopulations.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(02)00336-5</identifier><identifier>PMID: 12297400</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants ; Adjuvants, Immunologic - pharmacology ; Animals ; Biological and medical sciences ; Biotechnology ; Female ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Interferon-gamma - analysis ; Interferon-gamma - biosynthesis ; Interferon-γ ; Interleukin-4 ; Interleukin-4 - analysis ; Interleukin-4 - biosynthesis ; Lymph Nodes - drug effects ; Lymph Nodes - pathology ; Lymphocyte Activation - drug effects ; Mice ; Mice, Inbred CBA ; Ovalbumin - immunology ; Peptidoglycan - pharmacology ; Peptidoglycan monomer ; Production of active biomolecules ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; Vaccins</subject><ispartof>Vaccine, 2002-10, Vol.20 (29), p.3543-3550</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-c9aebd982b8ddd1a6d812213573e5c113f30d44c3376d1a5a81ec893005054ab3</citedby><cites>FETCH-LOGICAL-c455t-c9aebd982b8ddd1a6d812213573e5c113f30d44c3376d1a5a81ec893005054ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0264-410X(02)00336-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64387</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13936464$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12297400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halassy Špoljar, Beata</creatorcontrib><creatorcontrib>Čimbora, Tamara</creatorcontrib><creatorcontrib>Hanzl-Dujmović, Ivana</creatorcontrib><creatorcontrib>Dojnović, Biserka</creatorcontrib><creatorcontrib>Sabioncello, Ante</creatorcontrib><creatorcontrib>Krstanović, Marina</creatorcontrib><creatorcontrib>Tomašić, Jelka</creatorcontrib><title>Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Peptidoglycan monomer (PGM) originating from
Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T cell activation and cytokine networks in response to OVA. OVA-specific proliferative response as well as interferon-γ (IFN-γ) and interleukin-4 (IL-4) secretion in lymph node cell cultures of immunised mice were studied. Due to pharmacokinetic properties of PGM, namely its fast metabolism and excretion, special emphasis was on choosing the appropriate time for lymph node removal and duration of cell cultivation for each cytokine. PGM treatment in addition to OVA resulted in an increase of lymph node cellularity, stimulation of OVA-specific IFN-γ and IL-4 production as well as of OVA-specific proliferative response. Results demonstrate that PGM stimulated both Th1 and Th2 subpopulations.</description><subject>Adjuvants</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Interferon-gamma - analysis</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-γ</subject><subject>Interleukin-4</subject><subject>Interleukin-4 - analysis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Lymph Nodes - drug effects</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Ovalbumin - immunology</subject><subject>Peptidoglycan - pharmacology</subject><subject>Peptidoglycan monomer</subject><subject>Production of active biomolecules</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccins</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EosvCTwD5AiqHwDi283GqUMVHpUocKIKb5R1PkKvEDnayUv893u6K3uA0Bz-v_foZxl4KeCdANO-_Qd2oSgn4eQ71WwApm0o_YhvRtbKqteges81f5Iw9y_kWALQU_VN2Juq6bxXAhv24CsO4UkDiceDW3a57G5bK4uL3xGeaF-_ir_EObeBTDHGixGPgeSb0g0d-w5HGkSfKcwyZMveF80jP2ZPBjplenOaWff_08ebyS3X99fPV5YfrCpXWS4W9pZ3ru3rXOeeEbVxXugmpW0kahZCDBKcUStk25VjbThB2vSw_Aa3sTm7Zm-O9c4q_V8qLmXw-VLKB4pqN6LRUGuoCnv8b1ACt1n0xuWX6iGKKOScazJz8ZNOdEWAO8s29fHMwa6A29_KNLrlXpyfW3UTuIXWyXYDXJ8BmtOOQbECfHzjZy0Y1qnAXR46Kub2nZDL6w46cT4SLcdH_p8oftmmgfg</recordid><startdate>20021004</startdate><enddate>20021004</enddate><creator>Halassy Špoljar, Beata</creator><creator>Čimbora, Tamara</creator><creator>Hanzl-Dujmović, Ivana</creator><creator>Dojnović, Biserka</creator><creator>Sabioncello, Ante</creator><creator>Krstanović, Marina</creator><creator>Tomašić, Jelka</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20021004</creationdate><title>Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice</title><author>Halassy Špoljar, Beata ; Čimbora, Tamara ; Hanzl-Dujmović, Ivana ; Dojnović, Biserka ; Sabioncello, Ante ; Krstanović, Marina ; Tomašić, Jelka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-c9aebd982b8ddd1a6d812213573e5c113f30d44c3376d1a5a81ec893005054ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adjuvants</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Interferon-gamma - analysis</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-γ</topic><topic>Interleukin-4</topic><topic>Interleukin-4 - analysis</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Lymph Nodes - drug effects</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Ovalbumin - immunology</topic><topic>Peptidoglycan - pharmacology</topic><topic>Peptidoglycan monomer</topic><topic>Production of active biomolecules</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halassy Špoljar, Beata</creatorcontrib><creatorcontrib>Čimbora, Tamara</creatorcontrib><creatorcontrib>Hanzl-Dujmović, Ivana</creatorcontrib><creatorcontrib>Dojnović, Biserka</creatorcontrib><creatorcontrib>Sabioncello, Ante</creatorcontrib><creatorcontrib>Krstanović, Marina</creatorcontrib><creatorcontrib>Tomašić, Jelka</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halassy Špoljar, Beata</au><au>Čimbora, Tamara</au><au>Hanzl-Dujmović, Ivana</au><au>Dojnović, Biserka</au><au>Sabioncello, Ante</au><au>Krstanović, Marina</au><au>Tomašić, Jelka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2002-10-04</date><risdate>2002</risdate><volume>20</volume><issue>29</issue><spage>3543</spage><epage>3550</epage><pages>3543-3550</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Peptidoglycan monomer (PGM) originating from
Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T cell activation and cytokine networks in response to OVA. OVA-specific proliferative response as well as interferon-γ (IFN-γ) and interleukin-4 (IL-4) secretion in lymph node cell cultures of immunised mice were studied. Due to pharmacokinetic properties of PGM, namely its fast metabolism and excretion, special emphasis was on choosing the appropriate time for lymph node removal and duration of cell cultivation for each cytokine. PGM treatment in addition to OVA resulted in an increase of lymph node cellularity, stimulation of OVA-specific IFN-γ and IL-4 production as well as of OVA-specific proliferative response. Results demonstrate that PGM stimulated both Th1 and Th2 subpopulations.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12297400</pmid><doi>10.1016/S0264-410X(02)00336-5</doi><tpages>8</tpages></addata></record> |
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| ispartof | Vaccine, 2002-10, Vol.20 (29), p.3543-3550 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); ProQuest Central UK/Ireland |
| subjects | Adjuvants Adjuvants, Immunologic - pharmacology Animals Biological and medical sciences Biotechnology Female Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Interferon-gamma - analysis Interferon-gamma - biosynthesis Interferon-γ Interleukin-4 Interleukin-4 - analysis Interleukin-4 - biosynthesis Lymph Nodes - drug effects Lymph Nodes - pathology Lymphocyte Activation - drug effects Mice Mice, Inbred CBA Ovalbumin - immunology Peptidoglycan - pharmacology Peptidoglycan monomer Production of active biomolecules T-Lymphocytes - drug effects T-Lymphocytes - immunology Vaccins |
| title | Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice |
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