Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
[Display omitted] It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, wh...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2017-01, Vol.27 (2), p.168-170 |
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| container_title | Bioorganic & medicinal chemistry letters |
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| creator | Zhang, Hao Li, Tianhu |
| description | [Display omitted]
It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes. |
| doi_str_mv | 10.1016/j.bmcl.2016.11.088 |
| format | Article |
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It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2016.11.088</identifier><identifier>PMID: 27956347</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>AFM ; Deoxyribonuclease I - chemistry ; DNA Topoisomerases, Type I - chemistry ; DNA, Superhelical - chemistry ; E. coli topo I ; Escherichia coli ; Histone H1 ; Histones - chemistry ; Humans ; Nucleosomes - chemistry ; Plasmid DNA ; Plasmids ; Polynucleosome</subject><ispartof>Bioorganic & medicinal chemistry letters, 2017-01, Vol.27 (2), p.168-170</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-ee8cd5007833fe31d3e0fdd79000b41e874e2799d3fa43d45641f4b1d4fffac93</citedby><cites>FETCH-LOGICAL-c400t-ee8cd5007833fe31d3e0fdd79000b41e874e2799d3fa43d45641f4b1d4fffac93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960894X16312616$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27956347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Li, Tianhu</creatorcontrib><title>Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>[Display omitted]
It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes.</description><subject>AFM</subject><subject>Deoxyribonuclease I - chemistry</subject><subject>DNA Topoisomerases, Type I - chemistry</subject><subject>DNA, Superhelical - chemistry</subject><subject>E. coli topo I</subject><subject>Escherichia coli</subject><subject>Histone H1</subject><subject>Histones - chemistry</subject><subject>Humans</subject><subject>Nucleosomes - chemistry</subject><subject>Plasmid DNA</subject><subject>Plasmids</subject><subject>Polynucleosome</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMouq7-AQ_So5fWSZN-gRdZ1BUW9KDgLXaTyZolbWrSiv57W1Y9epqBeeZl5iHkjEJCgeaX22TdSJukY59QmkBZ7pEZ5TmPGYdsn8ygyiEuK_5yRI5D2AJQDpwfkqO0qLKc8WJGXh89BmwlRk5HLW7q3nxgFIYOvXTGmnYTmTaqNxs_zTBM2JsJvWsxWtK4s3VojDIy6pz9agdp0QXXYCRd01n8xHBCDnRtA57-1Dl5vr15Wizj1cPd_eJ6FUsO0MeIpVQZQFEyppFRxRC0UkUFAGtOsSw4jldXiumaM8WznFPN11RxrXUtKzYnF7vczrv3AUMvGhMkWlu36IYgaJmleZ6zKh3RdIdK70LwqEXnTVP7L0FBTGbFVkxmxWRWUCpGs-PS-U_-sG5Q_a38qhyBqx2A45cfBr0I0kxmlfEoe6Gc-S__G9-Xi-o</recordid><startdate>20170115</startdate><enddate>20170115</enddate><creator>Zhang, Hao</creator><creator>Li, Tianhu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170115</creationdate><title>Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes</title><author>Zhang, Hao ; Li, Tianhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-ee8cd5007833fe31d3e0fdd79000b41e874e2799d3fa43d45641f4b1d4fffac93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>AFM</topic><topic>Deoxyribonuclease I - chemistry</topic><topic>DNA Topoisomerases, Type I - chemistry</topic><topic>DNA, Superhelical - chemistry</topic><topic>E. coli topo I</topic><topic>Escherichia coli</topic><topic>Histone H1</topic><topic>Histones - chemistry</topic><topic>Humans</topic><topic>Nucleosomes - chemistry</topic><topic>Plasmid DNA</topic><topic>Plasmids</topic><topic>Polynucleosome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Li, Tianhu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hao</au><au>Li, Tianhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2017-01-15</date><risdate>2017</risdate><volume>27</volume><issue>2</issue><spage>168</spage><epage>170</epage><pages>168-170</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>[Display omitted]
It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27956347</pmid><doi>10.1016/j.bmcl.2016.11.088</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | AFM Deoxyribonuclease I - chemistry DNA Topoisomerases, Type I - chemistry DNA, Superhelical - chemistry E. coli topo I Escherichia coli Histone H1 Histones - chemistry Humans Nucleosomes - chemistry Plasmid DNA Plasmids Polynucleosome |
| title | Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes |
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