New tools for redox biology: From imaging to manipulation
Redox reactions play a key role in maintaining essential biological processes. Deviations in redox pathways result in the development of various pathologies at cellular and organismal levels. Until recently, studies on transformations in the intracellular redox state have been significantly hampered...
Gespeichert in:
| Veröffentlicht in: | Free radical biology & medicine 2017-08, Vol.109, p.167-188 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 188 |
|---|---|
| container_issue | |
| container_start_page | 167 |
| container_title | Free radical biology & medicine |
| container_volume | 109 |
| creator | Bilan, Dmitry S. Belousov, Vsevolod V. |
| description | Redox reactions play a key role in maintaining essential biological processes. Deviations in redox pathways result in the development of various pathologies at cellular and organismal levels. Until recently, studies on transformations in the intracellular redox state have been significantly hampered in living systems. The genetically encoded indicators, based on fluorescent proteins, have provided new opportunities in biomedical research. The existing indicators already enable monitoring of cellular redox parameters in different processes including embryogenesis, aging, inflammation, tissue regeneration, and pathogenesis of various diseases. In this review, we summarize information about all genetically encoded redox indicators developed to date. We provide the description of each indicator and discuss its advantages and limitations, as well as points that need to be considered when choosing an indicator for a particular experiment. One chapter is devoted to the important discoveries that have been made by using genetically encoded redox indicators.
•Genetically encoded indicators for redox biology research.•Detailed characteristics of each group of indicators.•Examples of studies using redox indicators.
[Display omitted] |
| doi_str_mv | 10.1016/j.freeradbiomed.2016.12.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1852660778</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0891584916310929</els_id><sourcerecordid>1852660778</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-4eb5c8ef64076847063902fb668cb49ed11bb202f7a242c8a7fd25841c3513b73</originalsourceid><addsrcrecordid>eNqNkMlOwzAQQC0EomX5BRSJC5cEb_ECJ4RaQEJwgbOVOJPKVRIXO2X5e1y1PXDjNNLMm-0hdElwQTAR18uiDQChamrne2gKmpIFoQXG_ABNiZIs56UWh2iKlSZ5qbieoJMYlzgRJVPHaEKlZlqXfIr0C3xlo_ddzFofsgCN_87S4M4vfm6yefB95vpq4YZForK-Gtxq3VWj88MZOmqrLsL5Lp6i9_ns7f4xf359eLq_e84tU2zMOdSlVdAKjqVQXGLBNKZtLYSyNdfQEFLXNGVkRTm1qpJtQ9PNxLKSsFqyU3S1nbsK_mMNcTS9ixa6rhrAr6MhqqRCYClVQm-3qA0-xgCtWYV0ffgxBJuNO7M0f9yZjTtDqElmUvfFbtG63tT2vXtZCZhtAUjvfjoIJloHg4XGBbCjabz716JfVjCGjw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1852660778</pqid></control><display><type>article</type><title>New tools for redox biology: From imaging to manipulation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Bilan, Dmitry S. ; Belousov, Vsevolod V.</creator><creatorcontrib>Bilan, Dmitry S. ; Belousov, Vsevolod V.</creatorcontrib><description>Redox reactions play a key role in maintaining essential biological processes. Deviations in redox pathways result in the development of various pathologies at cellular and organismal levels. Until recently, studies on transformations in the intracellular redox state have been significantly hampered in living systems. The genetically encoded indicators, based on fluorescent proteins, have provided new opportunities in biomedical research. The existing indicators already enable monitoring of cellular redox parameters in different processes including embryogenesis, aging, inflammation, tissue regeneration, and pathogenesis of various diseases. In this review, we summarize information about all genetically encoded redox indicators developed to date. We provide the description of each indicator and discuss its advantages and limitations, as well as points that need to be considered when choosing an indicator for a particular experiment. One chapter is devoted to the important discoveries that have been made by using genetically encoded redox indicators.
•Genetically encoded indicators for redox biology research.•Detailed characteristics of each group of indicators.•Examples of studies using redox indicators.
[Display omitted]</description><identifier>ISSN: 0891-5849</identifier><identifier>EISSN: 1873-4596</identifier><identifier>DOI: 10.1016/j.freeradbiomed.2016.12.004</identifier><identifier>PMID: 27939954</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biosensing Techniques ; D-aminoacid oxidase ; Embryonic Development - genetics ; Fluorescence Resonance Energy Transfer ; Gene Expression ; Green Fluorescent Proteins - chemistry ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Humans ; HyPer ; Imaging ; Inflammation ; Luminescent Proteins - chemistry ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Models, Molecular ; Molecular Imaging - methods ; Molecular Probes - biosynthesis ; Molecular Probes - chemical synthesis ; Molecular Probes - metabolism ; NAD ; NADH ; NADPH ; Oxidation-Reduction ; Protein Structure, Secondary ; Reactive Oxygen Species - analysis ; Reactive Oxygen Species - metabolism ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Redox probes ; RoGFP</subject><ispartof>Free radical biology & medicine, 2017-08, Vol.109, p.167-188</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-4eb5c8ef64076847063902fb668cb49ed11bb202f7a242c8a7fd25841c3513b73</citedby><cites>FETCH-LOGICAL-c383t-4eb5c8ef64076847063902fb668cb49ed11bb202f7a242c8a7fd25841c3513b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0891584916310929$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27939954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bilan, Dmitry S.</creatorcontrib><creatorcontrib>Belousov, Vsevolod V.</creatorcontrib><title>New tools for redox biology: From imaging to manipulation</title><title>Free radical biology & medicine</title><addtitle>Free Radic Biol Med</addtitle><description>Redox reactions play a key role in maintaining essential biological processes. Deviations in redox pathways result in the development of various pathologies at cellular and organismal levels. Until recently, studies on transformations in the intracellular redox state have been significantly hampered in living systems. The genetically encoded indicators, based on fluorescent proteins, have provided new opportunities in biomedical research. The existing indicators already enable monitoring of cellular redox parameters in different processes including embryogenesis, aging, inflammation, tissue regeneration, and pathogenesis of various diseases. In this review, we summarize information about all genetically encoded redox indicators developed to date. We provide the description of each indicator and discuss its advantages and limitations, as well as points that need to be considered when choosing an indicator for a particular experiment. One chapter is devoted to the important discoveries that have been made by using genetically encoded redox indicators.
•Genetically encoded indicators for redox biology research.•Detailed characteristics of each group of indicators.•Examples of studies using redox indicators.
[Display omitted]</description><subject>Animals</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biosensing Techniques</subject><subject>D-aminoacid oxidase</subject><subject>Embryonic Development - genetics</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Gene Expression</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>HyPer</subject><subject>Imaging</subject><subject>Inflammation</subject><subject>Luminescent Proteins - chemistry</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Models, Molecular</subject><subject>Molecular Imaging - methods</subject><subject>Molecular Probes - biosynthesis</subject><subject>Molecular Probes - chemical synthesis</subject><subject>Molecular Probes - metabolism</subject><subject>NAD</subject><subject>NADH</subject><subject>NADPH</subject><subject>Oxidation-Reduction</subject><subject>Protein Structure, Secondary</subject><subject>Reactive Oxygen Species - analysis</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Redox probes</subject><subject>RoGFP</subject><issn>0891-5849</issn><issn>1873-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMlOwzAQQC0EomX5BRSJC5cEb_ECJ4RaQEJwgbOVOJPKVRIXO2X5e1y1PXDjNNLMm-0hdElwQTAR18uiDQChamrne2gKmpIFoQXG_ABNiZIs56UWh2iKlSZ5qbieoJMYlzgRJVPHaEKlZlqXfIr0C3xlo_ddzFofsgCN_87S4M4vfm6yefB95vpq4YZForK-Gtxq3VWj88MZOmqrLsL5Lp6i9_ns7f4xf359eLq_e84tU2zMOdSlVdAKjqVQXGLBNKZtLYSyNdfQEFLXNGVkRTm1qpJtQ9PNxLKSsFqyU3S1nbsK_mMNcTS9ixa6rhrAr6MhqqRCYClVQm-3qA0-xgCtWYV0ffgxBJuNO7M0f9yZjTtDqElmUvfFbtG63tT2vXtZCZhtAUjvfjoIJloHg4XGBbCjabz716JfVjCGjw</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Bilan, Dmitry S.</creator><creator>Belousov, Vsevolod V.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>New tools for redox biology: From imaging to manipulation</title><author>Bilan, Dmitry S. ; Belousov, Vsevolod V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-4eb5c8ef64076847063902fb668cb49ed11bb202f7a242c8a7fd25841c3513b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biosensing Techniques</topic><topic>D-aminoacid oxidase</topic><topic>Embryonic Development - genetics</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Gene Expression</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Humans</topic><topic>HyPer</topic><topic>Imaging</topic><topic>Inflammation</topic><topic>Luminescent Proteins - chemistry</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Models, Molecular</topic><topic>Molecular Imaging - methods</topic><topic>Molecular Probes - biosynthesis</topic><topic>Molecular Probes - chemical synthesis</topic><topic>Molecular Probes - metabolism</topic><topic>NAD</topic><topic>NADH</topic><topic>NADPH</topic><topic>Oxidation-Reduction</topic><topic>Protein Structure, Secondary</topic><topic>Reactive Oxygen Species - analysis</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Redox probes</topic><topic>RoGFP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bilan, Dmitry S.</creatorcontrib><creatorcontrib>Belousov, Vsevolod V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bilan, Dmitry S.</au><au>Belousov, Vsevolod V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New tools for redox biology: From imaging to manipulation</atitle><jtitle>Free radical biology & medicine</jtitle><addtitle>Free Radic Biol Med</addtitle><date>2017-08</date><risdate>2017</risdate><volume>109</volume><spage>167</spage><epage>188</epage><pages>167-188</pages><issn>0891-5849</issn><eissn>1873-4596</eissn><abstract>Redox reactions play a key role in maintaining essential biological processes. Deviations in redox pathways result in the development of various pathologies at cellular and organismal levels. Until recently, studies on transformations in the intracellular redox state have been significantly hampered in living systems. The genetically encoded indicators, based on fluorescent proteins, have provided new opportunities in biomedical research. The existing indicators already enable monitoring of cellular redox parameters in different processes including embryogenesis, aging, inflammation, tissue regeneration, and pathogenesis of various diseases. In this review, we summarize information about all genetically encoded redox indicators developed to date. We provide the description of each indicator and discuss its advantages and limitations, as well as points that need to be considered when choosing an indicator for a particular experiment. One chapter is devoted to the important discoveries that have been made by using genetically encoded redox indicators.
•Genetically encoded indicators for redox biology research.•Detailed characteristics of each group of indicators.•Examples of studies using redox indicators.
[Display omitted]</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27939954</pmid><doi>10.1016/j.freeradbiomed.2016.12.004</doi><tpages>22</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0891-5849 |
| ispartof | Free radical biology & medicine, 2017-08, Vol.109, p.167-188 |
| issn | 0891-5849 1873-4596 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1852660778 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosensing Techniques D-aminoacid oxidase Embryonic Development - genetics Fluorescence Resonance Energy Transfer Gene Expression Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Humans HyPer Imaging Inflammation Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Models, Molecular Molecular Imaging - methods Molecular Probes - biosynthesis Molecular Probes - chemical synthesis Molecular Probes - metabolism NAD NADH NADPH Oxidation-Reduction Protein Structure, Secondary Reactive Oxygen Species - analysis Reactive Oxygen Species - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Redox probes RoGFP |
| title | New tools for redox biology: From imaging to manipulation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T17%3A35%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=New%20tools%20for%20redox%20biology:%20From%20imaging%20to%20manipulation&rft.jtitle=Free%20radical%20biology%20&%20medicine&rft.au=Bilan,%20Dmitry%20S.&rft.date=2017-08&rft.volume=109&rft.spage=167&rft.epage=188&rft.pages=167-188&rft.issn=0891-5849&rft.eissn=1873-4596&rft_id=info:doi/10.1016/j.freeradbiomed.2016.12.004&rft_dat=%3Cproquest_cross%3E1852660778%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1852660778&rft_id=info:pmid/27939954&rft_els_id=S0891584916310929&rfr_iscdi=true |