Testin is a tumor suppressor in non-small cell lung cancer
The Testin gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in...
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| Veröffentlicht in: | Oncology reports 2017-02, Vol.37 (2), p.1027-1035 |
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| creator | Wang, Ming Wang, Qian Peng, Wen-Jia Hu, Jun-Feng Wang, Zu-Yi Liu, Hao Huang, Li-Nian |
| description | The Testin gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in non-small cell lung cancer (NSCLC). In NSCLC cell lines, we observed lower expression of Testin compared to that noted in normal human bronchial epithelial cells. MTT assays, flow cytometry, clonogenic assay and invasion assay showed that the overexpression of the Testin gene inhibited cancer cell proliferation, invasion and colony formation. In tumor xenograft models, Testin markedly inhibited lung cancer cell xenograft formation and growth in athymic nude mice. Taken together, these data suggest that Testin plays an important role in the development and progression of NSCLC. Testin may be an effective novel target in NSCLC prevention and treatment. |
| doi_str_mv | 10.3892/or.2016.5316 |
| format | Article |
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It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in non-small cell lung cancer (NSCLC). In NSCLC cell lines, we observed lower expression of Testin compared to that noted in normal human bronchial epithelial cells. MTT assays, flow cytometry, clonogenic assay and invasion assay showed that the overexpression of the Testin gene inhibited cancer cell proliferation, invasion and colony formation. In tumor xenograft models, Testin markedly inhibited lung cancer cell xenograft formation and growth in athymic nude mice. Taken together, these data suggest that Testin plays an important role in the development and progression of NSCLC. Testin may be an effective novel target in NSCLC prevention and treatment.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2016.5316</identifier><identifier>PMID: 28000866</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; Animals ; Bone marrow ; Breast cancer ; Cancer therapies ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - pathology ; Care and treatment ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation - genetics ; Cytoskeletal Proteins - genetics ; Cytoskeletal Proteins - metabolism ; Development and progression ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genes, Tumor Suppressor ; Genetic aspects ; Health aspects ; Humans ; invasion ; Kinases ; LIM Domain Proteins - genetics ; LIM Domain Proteins - metabolism ; Lung cancer ; Lung cancer, Non-small cell ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Lymphoma ; Medical research ; Metastasis ; Mice, Inbred BALB C ; Mortality ; non-small cell lung cancer ; proliferation ; Proteins ; Studies ; Testin gene ; Tumor suppressor genes ; Xenograft Model Antitumor Assays</subject><ispartof>Oncology reports, 2017-02, Vol.37 (2), p.1027-1035</ispartof><rights>Copyright © 2017, Spandidos Publications</rights><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-7718d127da11534179f372476b0b9422efb2a118afde04e4c1656ff90b41f49f3</citedby><cites>FETCH-LOGICAL-c447t-7718d127da11534179f372476b0b9422efb2a118afde04e4c1656ff90b41f49f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28000866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ming</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Peng, Wen-Jia</creatorcontrib><creatorcontrib>Hu, Jun-Feng</creatorcontrib><creatorcontrib>Wang, Zu-Yi</creatorcontrib><creatorcontrib>Liu, Hao</creatorcontrib><creatorcontrib>Huang, Li-Nian</creatorcontrib><title>Testin is a tumor suppressor in non-small cell lung cancer</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>The Testin gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in non-small cell lung cancer (NSCLC). In NSCLC cell lines, we observed lower expression of Testin compared to that noted in normal human bronchial epithelial cells. MTT assays, flow cytometry, clonogenic assay and invasion assay showed that the overexpression of the Testin gene inhibited cancer cell proliferation, invasion and colony formation. In tumor xenograft models, Testin markedly inhibited lung cancer cell xenograft formation and growth in athymic nude mice. Taken together, these data suggest that Testin plays an important role in the development and progression of NSCLC. Testin may be an effective novel target in NSCLC prevention and treatment.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Bone marrow</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Development and progression</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes, Tumor Suppressor</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>invasion</subject><subject>Kinases</subject><subject>LIM Domain Proteins - genetics</subject><subject>LIM Domain Proteins - metabolism</subject><subject>Lung cancer</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Lymphoma</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>Mice, Inbred BALB C</subject><subject>Mortality</subject><subject>non-small cell lung cancer</subject><subject>proliferation</subject><subject>Proteins</subject><subject>Studies</subject><subject>Testin gene</subject><subject>Tumor suppressor genes</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0cFr1zAUB_AgipvTm2cpCOLB_HwvSZvG2xhOhYGXCd5C2iZbR5vUvPaw_96UzU29JIH34eULX8ZeIxxka8THlA8CsDnUEpsn7Bi1QS6UxKflDQK5lPXPI_aC6AZAaGjMc3YkWgBom-aYfbr0tI6xGqly1brNKVe0LUv2ROVZBjFFTrObpqr35Zi2eFX1LvY-v2TPgpvIv7q_T9iP88-XZ1_5xfcv385OL3ivlF651tgOKPTgEGupSr4gtVC66aAzSggfOlFGrQuDB-VVj03dhGCgUxhUwSfs_d3eJadfW4lr55H2MC76tJHFtkYJYFos9O1_9CZtOZZ0Fo1CYRAFPKorN3k7xpDW7Pp9qT1VhUhTG1HUu7_UtXfTek1p2tYxRfoXfriDfU5E2Qe75HF2-dYi2L0im7LdK7J7RYW_uc-4dbMfHvCfTh4_psXFYRwSPZiUudQcBC_NavkbiAKT-g</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Wang, Ming</creator><creator>Wang, Qian</creator><creator>Peng, Wen-Jia</creator><creator>Hu, Jun-Feng</creator><creator>Wang, Zu-Yi</creator><creator>Liu, Hao</creator><creator>Huang, Li-Nian</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>Testin is a tumor suppressor in non-small cell lung cancer</title><author>Wang, Ming ; Wang, Qian ; Peng, Wen-Jia ; Hu, Jun-Feng ; Wang, Zu-Yi ; Liu, Hao ; Huang, Li-Nian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-7718d127da11534179f372476b0b9422efb2a118afde04e4c1656ff90b41f49f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Bone marrow</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Development and progression</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes, Tumor Suppressor</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>invasion</topic><topic>Kinases</topic><topic>LIM Domain Proteins - genetics</topic><topic>LIM Domain Proteins - metabolism</topic><topic>Lung cancer</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Lymphoma</topic><topic>Medical research</topic><topic>Metastasis</topic><topic>Mice, Inbred BALB C</topic><topic>Mortality</topic><topic>non-small cell lung cancer</topic><topic>proliferation</topic><topic>Proteins</topic><topic>Studies</topic><topic>Testin gene</topic><topic>Tumor suppressor genes</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ming</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Peng, Wen-Jia</creatorcontrib><creatorcontrib>Hu, Jun-Feng</creatorcontrib><creatorcontrib>Wang, Zu-Yi</creatorcontrib><creatorcontrib>Liu, Hao</creatorcontrib><creatorcontrib>Huang, Li-Nian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ming</au><au>Wang, Qian</au><au>Peng, Wen-Jia</au><au>Hu, Jun-Feng</au><au>Wang, Zu-Yi</au><au>Liu, Hao</au><au>Huang, Li-Nian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testin is a tumor suppressor in non-small cell lung cancer</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>37</volume><issue>2</issue><spage>1027</spage><epage>1035</epage><pages>1027-1035</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>The Testin gene was previously identified in the fragile chromosomal region FRA7G at 7q31.2. It has been implicated in several types of cancers including prostate, ovarian, breast and gastric cancer. In the present study, we investigated the function of the candidate tumor-suppressor Testin gene in non-small cell lung cancer (NSCLC). In NSCLC cell lines, we observed lower expression of Testin compared to that noted in normal human bronchial epithelial cells. MTT assays, flow cytometry, clonogenic assay and invasion assay showed that the overexpression of the Testin gene inhibited cancer cell proliferation, invasion and colony formation. In tumor xenograft models, Testin markedly inhibited lung cancer cell xenograft formation and growth in athymic nude mice. Taken together, these data suggest that Testin plays an important role in the development and progression of NSCLC. Testin may be an effective novel target in NSCLC prevention and treatment.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>28000866</pmid><doi>10.3892/or.2016.5316</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Angiogenesis Animals Bone marrow Breast cancer Cancer therapies Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Care and treatment Cell cycle Cell growth Cell Line, Tumor Cell Movement - genetics Cell Proliferation - genetics Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Development and progression Female Gene expression Gene Expression Regulation, Neoplastic Genes, Tumor Suppressor Genetic aspects Health aspects Humans invasion Kinases LIM Domain Proteins - genetics LIM Domain Proteins - metabolism Lung cancer Lung cancer, Non-small cell Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Lymphoma Medical research Metastasis Mice, Inbred BALB C Mortality non-small cell lung cancer proliferation Proteins Studies Testin gene Tumor suppressor genes Xenograft Model Antitumor Assays |
| title | Testin is a tumor suppressor in non-small cell lung cancer |
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