Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway
To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia. The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electro...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2016-11, Vol.37 (11), p.982 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Liu, M H Yang, L Liu, X J Nie, Z Y Luo, J M |
| description | To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia.
The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electrotransfection. RT-PCR was used to detect the miRNA-21 expression changes. Cell proliferation and apoptosis were determined by using MTT and flow cytometry. Western-blot were used to detect the protein expression changes of PTEN, PI3K and p-AKT respectively.
The relative expression of miRNA-21 in experimental group was (8.070 ± 5.138)% at 24 hours, which was lower than control groups ( |
| doi_str_mv | 10.3760/cma.j.issn.0253-2727.2016.11.011 |
| format | Article |
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The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electrotransfection. RT-PCR was used to detect the miRNA-21 expression changes. Cell proliferation and apoptosis were determined by using MTT and flow cytometry. Western-blot were used to detect the protein expression changes of PTEN, PI3K and p-AKT respectively.
The relative expression of miRNA-21 in experimental group was (8.070 ± 5.138)% at 24 hours, which was lower than control groups (
<0.05). The apoptotic rate of (13.370±0.250)% at 24 hours in experimental group was obviously higher than control groups. The cellular proliferation were significantly different at 24 hours. The proliferation inhibition rate was (8.1±0.9)% at 24 hours, which was up to (43.1±2.1)% at 60 hours, but the control groups showed no difference</description><identifier>ISSN: 0253-2727</identifier><identifier>DOI: 10.3760/cma.j.issn.0253-2727.2016.11.011</identifier><identifier>PMID: 27995885</identifier><language>chi</language><publisher>China</publisher><subject>Apoptosis ; Cell Cycle ; Cell Proliferation ; Down-Regulation ; Humans ; K562 Cells ; Leukemia ; MicroRNAs - physiology ; Phosphatidylinositol 3-Kinases - metabolism ; Proto-Oncogene Proteins c-akt - metabolism ; PTEN Phosphohydrolase ; Signal Transduction ; Up-Regulation</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2016-11, Vol.37 (11), p.982</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27995885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, M H</creatorcontrib><creatorcontrib>Yang, L</creatorcontrib><creatorcontrib>Liu, X J</creatorcontrib><creatorcontrib>Nie, Z Y</creatorcontrib><creatorcontrib>Luo, J M</creatorcontrib><title>Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia.
The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electrotransfection. RT-PCR was used to detect the miRNA-21 expression changes. Cell proliferation and apoptosis were determined by using MTT and flow cytometry. Western-blot were used to detect the protein expression changes of PTEN, PI3K and p-AKT respectively.
The relative expression of miRNA-21 in experimental group was (8.070 ± 5.138)% at 24 hours, which was lower than control groups (
<0.05). The apoptotic rate of (13.370±0.250)% at 24 hours in experimental group was obviously higher than control groups. The cellular proliferation were significantly different at 24 hours. The proliferation inhibition rate was (8.1±0.9)% at 24 hours, which was up to (43.1±2.1)% at 60 hours, but the control groups showed no difference</description><subject>Apoptosis</subject><subject>Cell Cycle</subject><subject>Cell Proliferation</subject><subject>Down-Regulation</subject><subject>Humans</subject><subject>K562 Cells</subject><subject>Leukemia</subject><subject>MicroRNAs - physiology</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>PTEN Phosphohydrolase</subject><subject>Signal Transduction</subject><subject>Up-Regulation</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLw0AAhPeg2FL7F2SPvSTdR_aRYylVS0otEs9ht9lNVvIym1D6741YPc0wfAzDALDCKKSCo_W5VuFn6LxvQkQYDYggIiQI8xDjEGF8B-b_-QwsvXcaMUy5pBQ9gBkRccykZHOgU9UXZjA59GPX9WYi2wa2Ftbu_bgJCIauKZ12A0wYJ_BsqsrDom8vQwmHsm_HooSndHcMTnuarDdJCr0rGlW5poCdGsqLuj6Ce6sqb5Y3XYCP5126fQ0Oby_77eYQdJjwIRDSxpbIydOIcS5yaxiWhCCuI2MVnWwU0Tw6kzxWVjFpdK6Z1EZSFjON6AKsfnu7vv0ajR-y2vmfwaox7egzLBkmsSAontCnGzrq2uRZ17ta9dfs7xf6Db3JZo0</recordid><startdate>20161114</startdate><enddate>20161114</enddate><creator>Liu, M H</creator><creator>Yang, L</creator><creator>Liu, X J</creator><creator>Nie, Z Y</creator><creator>Luo, J M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20161114</creationdate><title>Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway</title><author>Liu, M H ; Yang, L ; Liu, X J ; Nie, Z Y ; Luo, J M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-78f9f28126345667dfe5182206b4efa3822443d4c2d9afa58ebdb58be83595b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2016</creationdate><topic>Apoptosis</topic><topic>Cell Cycle</topic><topic>Cell Proliferation</topic><topic>Down-Regulation</topic><topic>Humans</topic><topic>K562 Cells</topic><topic>Leukemia</topic><topic>MicroRNAs - physiology</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>PTEN Phosphohydrolase</topic><topic>Signal Transduction</topic><topic>Up-Regulation</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, M H</creatorcontrib><creatorcontrib>Yang, L</creatorcontrib><creatorcontrib>Liu, X J</creatorcontrib><creatorcontrib>Nie, Z Y</creatorcontrib><creatorcontrib>Luo, J M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, M H</au><au>Yang, L</au><au>Liu, X J</au><au>Nie, Z Y</au><au>Luo, J M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2016-11-14</date><risdate>2016</risdate><volume>37</volume><issue>11</issue><spage>982</spage><pages>982-</pages><issn>0253-2727</issn><abstract>To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia.
The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electrotransfection. RT-PCR was used to detect the miRNA-21 expression changes. Cell proliferation and apoptosis were determined by using MTT and flow cytometry. Western-blot were used to detect the protein expression changes of PTEN, PI3K and p-AKT respectively.
The relative expression of miRNA-21 in experimental group was (8.070 ± 5.138)% at 24 hours, which was lower than control groups (
<0.05). The apoptotic rate of (13.370±0.250)% at 24 hours in experimental group was obviously higher than control groups. The cellular proliferation were significantly different at 24 hours. The proliferation inhibition rate was (8.1±0.9)% at 24 hours, which was up to (43.1±2.1)% at 60 hours, but the control groups showed no difference</abstract><cop>China</cop><pmid>27995885</pmid><doi>10.3760/cma.j.issn.0253-2727.2016.11.011</doi></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2016-11, Vol.37 (11), p.982 |
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| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Apoptosis Cell Cycle Cell Proliferation Down-Regulation Humans K562 Cells Leukemia MicroRNAs - physiology Phosphatidylinositol 3-Kinases - metabolism Proto-Oncogene Proteins c-akt - metabolism PTEN Phosphohydrolase Signal Transduction Up-Regulation |
| title | Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway |
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