The pH of the Plasmodium falciparum digestive vacuole: holy grail or dead-end trail?
The maintenance of acidic pH in the digestive vacuole of the malaria parasite is thought to be crucial to the digestion of host cell haemoglobin and the subsequent process of heme detoxification. It may also be important in the mode of action of chloroquine and in the mechanism of resistance to the...
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| Veröffentlicht in: | Trends in parasitology 2002-10, Vol.18 (10), p.441-444 |
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| creator | Spiller, David G Bray, Patrick G Hughes, Ruth H Ward, Stephen A White, Michael R.H |
| description | The maintenance of acidic pH in the digestive vacuole of the malaria parasite is thought to be crucial to the digestion of host cell haemoglobin and the subsequent process of heme detoxification. It may also be important in the mode of action of chloroquine and in the mechanism of resistance to the drug. Obtaining a definitive measurement of digestive vacuole pH has been surprisingly difficult. Some of the techniques for the measurement of pH in acid vesicles are outlined here along with some key aspects that are specific to malaria parasites. The use of acridine orange and dextran-tagged dyes as probes for the measurement of digestive vacuole pH has proved problematic, yet some surprising findings have emerged from work with these compounds.
Recent studies of P. faciparum digestive vacuole pH have raised more questions than answers, but have hinted that this organelle may be a dump for waste products, rather than a metabolic headquarters. |
| doi_str_mv | 10.1016/S1471-4922(02)02365-6 |
| format | Article |
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Recent studies of P. faciparum digestive vacuole pH have raised more questions than answers, but have hinted that this organelle may be a dump for waste products, rather than a metabolic headquarters.</description><subject>acridine orange</subject><subject>Acridine Orange - metabolism</subject><subject>Animals</subject><subject>Antimalarials - metabolism</subject><subject>Antimalarials - pharmacology</subject><subject>Benzopyrans</subject><subject>Biological and medical sciences</subject><subject>chloroquine</subject><subject>Chloroquine - metabolism</subject><subject>Chloroquine - pharmacology</subject><subject>dextran</subject><subject>digestive vacuole</subject><subject>Drug Resistance</subject><subject>Erythrocytes - metabolism</subject><subject>Erythrocytes - parasitology</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>malaria</subject><subject>Malaria, Falciparum - drug therapy</subject><subject>Malaria, Falciparum - parasitology</subject><subject>Plasmodium falciparum - metabolism</subject><subject>Protozoa</subject><subject>Vacuoles - metabolism</subject><issn>1471-4922</issn><issn>1471-5007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9L3TAUx4Ns-HN_gpKXje2hepI2TeqLDHFzICh49xzS5FQj7c01aS_43y_1FnwcBPLl5HOSwyeEnDI4Z8Dqi0dWSVZUDeffgf8AXtaiqPfI4XtZAMhPS56RA3KU0gsAE1I2--SA8VJK0VSHZLV6Rrq5paGjY04PvUlDcH4aaGd66zcm5uj8E6bRb5FujZ1Cj5f0OfRv9Cka39MQqUPjClw7Os6VqxPyOXcn_LLsx-Tvr5vV9W1xd__7z_XPu8JWnI9F2dVQcSdrKa2QIGpumVIOoFVVkw_aqnRWdZ0Q2FpwbWOEKhWXtgZrO8XLY_Jtd-8mhtcpj6gHnyz2vVljmJJmSoBqeJlBsQNtDClF7PQm-sHEN81Azzr1u049u9Iwr6xT17nvbHlgagd0H12Lvwx8XQCTrOm7aNbWpw-ubFj-C5a5qx2HWcfWY9TJelxbdD6iHbUL_j-j_AMsQJBQ</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Spiller, David G</creator><creator>Bray, Patrick G</creator><creator>Hughes, Ruth H</creator><creator>Ward, Stephen A</creator><creator>White, Michael R.H</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20021001</creationdate><title>The pH of the Plasmodium falciparum digestive vacuole: holy grail or dead-end trail?</title><author>Spiller, David G ; Bray, Patrick G ; Hughes, Ruth H ; Ward, Stephen A ; White, Michael R.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-3f6042d7677c570562c188d00b84942db43dc8ff55ebc0db9a583827c60ccf823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>acridine orange</topic><topic>Acridine Orange - metabolism</topic><topic>Animals</topic><topic>Antimalarials - metabolism</topic><topic>Antimalarials - pharmacology</topic><topic>Benzopyrans</topic><topic>Biological and medical sciences</topic><topic>chloroquine</topic><topic>Chloroquine - metabolism</topic><topic>Chloroquine - pharmacology</topic><topic>dextran</topic><topic>digestive vacuole</topic><topic>Drug Resistance</topic><topic>Erythrocytes - metabolism</topic><topic>Erythrocytes - parasitology</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>malaria</topic><topic>Malaria, Falciparum - drug therapy</topic><topic>Malaria, Falciparum - parasitology</topic><topic>Plasmodium falciparum - metabolism</topic><topic>Protozoa</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spiller, David G</creatorcontrib><creatorcontrib>Bray, Patrick G</creatorcontrib><creatorcontrib>Hughes, Ruth H</creatorcontrib><creatorcontrib>Ward, Stephen A</creatorcontrib><creatorcontrib>White, Michael R.H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Trends in parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spiller, David G</au><au>Bray, Patrick G</au><au>Hughes, Ruth H</au><au>Ward, Stephen A</au><au>White, Michael R.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The pH of the Plasmodium falciparum digestive vacuole: holy grail or dead-end trail?</atitle><jtitle>Trends in parasitology</jtitle><addtitle>Trends Parasitol</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>18</volume><issue>10</issue><spage>441</spage><epage>444</epage><pages>441-444</pages><issn>1471-4922</issn><eissn>1471-5007</eissn><abstract>The maintenance of acidic pH in the digestive vacuole of the malaria parasite is thought to be crucial to the digestion of host cell haemoglobin and the subsequent process of heme detoxification. It may also be important in the mode of action of chloroquine and in the mechanism of resistance to the drug. Obtaining a definitive measurement of digestive vacuole pH has been surprisingly difficult. Some of the techniques for the measurement of pH in acid vesicles are outlined here along with some key aspects that are specific to malaria parasites. The use of acridine orange and dextran-tagged dyes as probes for the measurement of digestive vacuole pH has proved problematic, yet some surprising findings have emerged from work with these compounds.
Recent studies of P. faciparum digestive vacuole pH have raised more questions than answers, but have hinted that this organelle may be a dump for waste products, rather than a metabolic headquarters.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12377594</pmid><doi>10.1016/S1471-4922(02)02365-6</doi><tpages>4</tpages></addata></record> |
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| subjects | acridine orange Acridine Orange - metabolism Animals Antimalarials - metabolism Antimalarials - pharmacology Benzopyrans Biological and medical sciences chloroquine Chloroquine - metabolism Chloroquine - pharmacology dextran digestive vacuole Drug Resistance Erythrocytes - metabolism Erythrocytes - parasitology Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Hemoglobins - metabolism Humans Hydrogen-Ion Concentration Life cycle. Host-agent relationship. Pathogenesis malaria Malaria, Falciparum - drug therapy Malaria, Falciparum - parasitology Plasmodium falciparum - metabolism Protozoa Vacuoles - metabolism |
| title | The pH of the Plasmodium falciparum digestive vacuole: holy grail or dead-end trail? |
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