Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats
Abstract Background Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia. Objective The purpose of this study w...
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| Veröffentlicht in: | Physical therapy 2016-11, Vol.96 (11), p.1791-1798 |
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| creator | Sasaki, Yuichi Sasaki, Masanori Kataoka-Sasaki, Yuko Nakazaki, Masahito Nagahama, Hiroshi Suzuki, Junpei Tateyama, Daiki Oka, Shinichi Namioka, Takahiro Namioka, Ai Onodera, Rie Mikami, Takeshi Wanibuchi, Masahiko Kakizawa, Masafumi Ishiai, Sumio Kocsis, Jeffery D. Honmou, Osamu |
| description | Abstract
Background
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia.
Objective
The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats.
Design
This was an experimental study.
Methods
A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test.
Results
Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group.
Limitations
A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.
Conclusions
The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model. |
| doi_str_mv | 10.2522/ptj.20150504 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1850786885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A474767866</galeid><oup_id>10.2522/ptj.20150504</oup_id><sourcerecordid>A474767866</sourcerecordid><originalsourceid>FETCH-LOGICAL-c733t-6fc655b0ceefd7f79af905c1a5e765ab32ef52ee77b3b0a046fa82cec98616f13</originalsourceid><addsrcrecordid>eNqV0s2LEzEUAPBBFLeu3jzLgAcVnJpkJsnMsZR1LVQXWj2HTPrSnTqT1ElG7H_vK-0qlbKs5JCQ_PLy8V6SvKRkzDhjH7ZxM2aEcsJJ8SgZUZ6XmZCseJyMCMlpVhGWXyTPQtgQQqgsqqfJBZM4YLwaJZvlzkG_bkx6ZS2YGFJv0wXc6rppm6hj412q3Sqdudjrn-D8EHBsh7BfQPoZAjhzu-t0my4jdOkU2jakExuhx4nef4e0celCx_A8eWJ1G-DFsb9Mvn28-jr9lM1vrmfTyTwzMs9jJqwRnNfEANiVtLLStiLcUM1BCq7rnIHlDEDKOq-JJoWwumQGTFUKKizNL5O3h7jb3v8YIETVNcHgtbQDvL6iJSeyFGXJH0CZEFLklCF9_Q_d-KF3-BBUucSg2P6qtW5BNc56_DazD6omhSykwIMFquyMWgNmQrfegW1w-sSPz3hsK-gac3bDu5MNaCL8ims9hKBmy8V_2C8PtuX1_L5HHq3xbQtrUJjy6c2pf3_wpvch9GDVtm863e8UJWpf6QorXd1VOvJXx3wMdQerP_iutBG8OQA_bO8P9RuR6f_K</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1837185858</pqid></control><display><type>article</type><title>Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Sasaki, Yuichi ; Sasaki, Masanori ; Kataoka-Sasaki, Yuko ; Nakazaki, Masahito ; Nagahama, Hiroshi ; Suzuki, Junpei ; Tateyama, Daiki ; Oka, Shinichi ; Namioka, Takahiro ; Namioka, Ai ; Onodera, Rie ; Mikami, Takeshi ; Wanibuchi, Masahiko ; Kakizawa, Masafumi ; Ishiai, Sumio ; Kocsis, Jeffery D. ; Honmou, Osamu</creator><creatorcontrib>Sasaki, Yuichi ; Sasaki, Masanori ; Kataoka-Sasaki, Yuko ; Nakazaki, Masahito ; Nagahama, Hiroshi ; Suzuki, Junpei ; Tateyama, Daiki ; Oka, Shinichi ; Namioka, Takahiro ; Namioka, Ai ; Onodera, Rie ; Mikami, Takeshi ; Wanibuchi, Masahiko ; Kakizawa, Masafumi ; Ishiai, Sumio ; Kocsis, Jeffery D. ; Honmou, Osamu</creatorcontrib><description>Abstract
Background
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia.
Objective
The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats.
Design
This was an experimental study.
Methods
A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test.
Results
Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group.
Limitations
A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.
Conclusions
The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model.</description><identifier>ISSN: 0031-9023</identifier><identifier>EISSN: 1538-6724</identifier><identifier>DOI: 10.2522/ptj.20150504</identifier><identifier>PMID: 27174259</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Animals ; Bone marrow ; Care and treatment ; Carotid arteries ; Disease Models, Animal ; Fitness equipment ; Infusions, Intravenous ; Ischemia ; Mesenchymal Stem Cell Transplantation ; Methods ; Nervous system ; NMR ; Nuclear magnetic resonance ; Patient outcomes ; Physical Conditioning, Animal ; Rats ; Rats, Sprague-Dawley ; Rehabilitation ; Rodents ; Stem cell transplantation ; Stem cells ; Stroke ; Stroke (Disease) ; Stroke Rehabilitation - methods ; Studies ; Transplantation ; Veins & arteries</subject><ispartof>Physical therapy, 2016-11, Vol.96 (11), p.1791-1798</ispartof><rights>2016 American Physical Therapy Association 2016</rights><rights>2016 American Physical Therapy Association.</rights><rights>COPYRIGHT 2016 Oxford University Press</rights><rights>COPYRIGHT 2016 Oxford University Press</rights><rights>Copyright AMERICAN PHYSICAL THERAPY ASSOCIATION Nov 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c733t-6fc655b0ceefd7f79af905c1a5e765ab32ef52ee77b3b0a046fa82cec98616f13</citedby><cites>FETCH-LOGICAL-c733t-6fc655b0ceefd7f79af905c1a5e765ab32ef52ee77b3b0a046fa82cec98616f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27174259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasaki, Yuichi</creatorcontrib><creatorcontrib>Sasaki, Masanori</creatorcontrib><creatorcontrib>Kataoka-Sasaki, Yuko</creatorcontrib><creatorcontrib>Nakazaki, Masahito</creatorcontrib><creatorcontrib>Nagahama, Hiroshi</creatorcontrib><creatorcontrib>Suzuki, Junpei</creatorcontrib><creatorcontrib>Tateyama, Daiki</creatorcontrib><creatorcontrib>Oka, Shinichi</creatorcontrib><creatorcontrib>Namioka, Takahiro</creatorcontrib><creatorcontrib>Namioka, Ai</creatorcontrib><creatorcontrib>Onodera, Rie</creatorcontrib><creatorcontrib>Mikami, Takeshi</creatorcontrib><creatorcontrib>Wanibuchi, Masahiko</creatorcontrib><creatorcontrib>Kakizawa, Masafumi</creatorcontrib><creatorcontrib>Ishiai, Sumio</creatorcontrib><creatorcontrib>Kocsis, Jeffery D.</creatorcontrib><creatorcontrib>Honmou, Osamu</creatorcontrib><title>Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats</title><title>Physical therapy</title><addtitle>Phys Ther</addtitle><description>Abstract
Background
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia.
Objective
The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats.
Design
This was an experimental study.
Methods
A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test.
Results
Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group.
Limitations
A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.
Conclusions
The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model.</description><subject>Animals</subject><subject>Bone marrow</subject><subject>Care and treatment</subject><subject>Carotid arteries</subject><subject>Disease Models, Animal</subject><subject>Fitness equipment</subject><subject>Infusions, Intravenous</subject><subject>Ischemia</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Methods</subject><subject>Nervous system</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Patient outcomes</subject><subject>Physical Conditioning, Animal</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rehabilitation</subject><subject>Rodents</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stroke</subject><subject>Stroke (Disease)</subject><subject>Stroke Rehabilitation - methods</subject><subject>Studies</subject><subject>Transplantation</subject><subject>Veins & 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Yuichi</creator><creator>Sasaki, Masanori</creator><creator>Kataoka-Sasaki, Yuko</creator><creator>Nakazaki, Masahito</creator><creator>Nagahama, Hiroshi</creator><creator>Suzuki, Junpei</creator><creator>Tateyama, Daiki</creator><creator>Oka, Shinichi</creator><creator>Namioka, Takahiro</creator><creator>Namioka, Ai</creator><creator>Onodera, Rie</creator><creator>Mikami, Takeshi</creator><creator>Wanibuchi, Masahiko</creator><creator>Kakizawa, Masafumi</creator><creator>Ishiai, Sumio</creator><creator>Kocsis, Jeffery D.</creator><creator>Honmou, Osamu</creator><general>Oxford University 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Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats</title><author>Sasaki, Yuichi ; Sasaki, Masanori ; Kataoka-Sasaki, Yuko ; Nakazaki, Masahito ; Nagahama, Hiroshi ; Suzuki, Junpei ; Tateyama, Daiki ; Oka, Shinichi ; Namioka, Takahiro ; Namioka, Ai ; Onodera, Rie ; Mikami, Takeshi ; Wanibuchi, Masahiko ; Kakizawa, Masafumi ; Ishiai, Sumio ; Kocsis, Jeffery D. ; Honmou, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c733t-6fc655b0ceefd7f79af905c1a5e765ab32ef52ee77b3b0a046fa82cec98616f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bone marrow</topic><topic>Care and treatment</topic><topic>Carotid arteries</topic><topic>Disease Models, Animal</topic><topic>Fitness equipment</topic><topic>Infusions, Intravenous</topic><topic>Ischemia</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Methods</topic><topic>Nervous system</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Patient outcomes</topic><topic>Physical Conditioning, Animal</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rehabilitation</topic><topic>Rodents</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stroke</topic><topic>Stroke (Disease)</topic><topic>Stroke Rehabilitation - methods</topic><topic>Studies</topic><topic>Transplantation</topic><topic>Veins & arteries</topic><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Yuichi</creatorcontrib><creatorcontrib>Sasaki, Masanori</creatorcontrib><creatorcontrib>Kataoka-Sasaki, Yuko</creatorcontrib><creatorcontrib>Nakazaki, Masahito</creatorcontrib><creatorcontrib>Nagahama, Hiroshi</creatorcontrib><creatorcontrib>Suzuki, Junpei</creatorcontrib><creatorcontrib>Tateyama, Daiki</creatorcontrib><creatorcontrib>Oka, Shinichi</creatorcontrib><creatorcontrib>Namioka, Takahiro</creatorcontrib><creatorcontrib>Namioka, Ai</creatorcontrib><creatorcontrib>Onodera, Rie</creatorcontrib><creatorcontrib>Mikami, Takeshi</creatorcontrib><creatorcontrib>Wanibuchi, Masahiko</creatorcontrib><creatorcontrib>Kakizawa, Masafumi</creatorcontrib><creatorcontrib>Ishiai, Sumio</creatorcontrib><creatorcontrib>Kocsis, Jeffery D.</creatorcontrib><creatorcontrib>Honmou, Osamu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health 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Masahiko</au><au>Kakizawa, Masafumi</au><au>Ishiai, Sumio</au><au>Kocsis, Jeffery D.</au><au>Honmou, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats</atitle><jtitle>Physical therapy</jtitle><addtitle>Phys Ther</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>96</volume><issue>11</issue><spage>1791</spage><epage>1798</epage><pages>1791-1798</pages><issn>0031-9023</issn><eissn>1538-6724</eissn><abstract>Abstract
Background
Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat stroke models. Rehabilitation therapy through physical exercise also provides therapeutic efficacy for cerebral ischemia.
Objective
The purpose of this study was to investigate whether synergic effects of daily rehabilitation and intravenous infusion of MSCs has therapeutic effects after stroke in rats.
Design
This was an experimental study.
Methods
A permanent middle cerebral artery occlusion (MCAO) was induced by intraluminal vascular occlusion with a microfilament. Four experimental groups were studied: group 1 (vehicle only, n=10), group 2 (vehicle + exercise, n=10), group 3 (MSCs only, n=10), and group 4 (MSCs + exercise, n=10). Rat MSCs were intravenously infused at 6 hours after MCAO, and the rats received daily rehabilitation with treadmill running exercise for 20 minutes. Lesion size was assessed at 1, 14, and 35 days using magnetic resonance imaging. Functional outcome was assessed using the Limb Placement Test.
Results
Both combined therapy and MSC infusion reduced lesion volume, induced synaptogenesis, and elicited functional improvement compared with the groups without MSC infusion, but the effect was greater in the combined therapy group.
Limitations
A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.
Conclusions
The data indicate that the combined therapy of daily rehabilitation and intravenous infusion of MSCs improved functional outcome in a rat MCAO model.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>27174259</pmid><doi>10.2522/ptj.20150504</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9023 |
| ispartof | Physical therapy, 2016-11, Vol.96 (11), p.1791-1798 |
| issn | 0031-9023 1538-6724 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1850786885 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Bone marrow Care and treatment Carotid arteries Disease Models, Animal Fitness equipment Infusions, Intravenous Ischemia Mesenchymal Stem Cell Transplantation Methods Nervous system NMR Nuclear magnetic resonance Patient outcomes Physical Conditioning, Animal Rats Rats, Sprague-Dawley Rehabilitation Rodents Stem cell transplantation Stem cells Stroke Stroke (Disease) Stroke Rehabilitation - methods Studies Transplantation Veins & arteries |
| title | Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats |
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