Estrogen receptor α in mouse splenic lymphocytes: possible involvement in immunity
To elucidate relevance of estrogens to immune responses, we investigated whether estrogen receptor α (ER α) exists in mouse splenic B cells and T cells and the effect of 17β-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ER α was identified in both male and female mou...
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| Veröffentlicht in: | Toxicology letters 2002-07, Vol.133 (2), p.221-229 |
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| creator | Sakazaki, Humitoshi Ueno, Hitoshi Nakamuro, Katsuhiko |
| description | To elucidate relevance of estrogens to immune responses, we investigated whether estrogen receptor
α (ER
α) exists in mouse splenic B cells and T cells and the effect of 17β-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ER
α was identified in both male and female mouse splenic cells using RT-PCR. Crude splenic cells were stained with anti-ER antibody, and the distribution of ER
α in the splenic B cells and part of the splenic T cells was confirmed by flow cytometry. 17β-Estradiol inhibited B cell mitogenesis at the concentration of 10
−8 M and T cell mitogenesis at the concentration of 10
−6 M. Some EDCs, diethylstilbestrol, bisphenol A,
p-nonylphenol and di-2-ethylhexylphthalate, suppressed lymphocyte mitogenesis at the concentration of 10
−6–10
−5 M. We therefore suggest that estrogen may suppress lymphocyte mitogenesis through ER
α in B and T cells. |
| doi_str_mv | 10.1016/S0378-4274(02)00203-5 |
| format | Article |
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α (ER
α) exists in mouse splenic B cells and T cells and the effect of 17β-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ER
α was identified in both male and female mouse splenic cells using RT-PCR. Crude splenic cells were stained with anti-ER antibody, and the distribution of ER
α in the splenic B cells and part of the splenic T cells was confirmed by flow cytometry. 17β-Estradiol inhibited B cell mitogenesis at the concentration of 10
−8 M and T cell mitogenesis at the concentration of 10
−6 M. Some EDCs, diethylstilbestrol, bisphenol A,
p-nonylphenol and di-2-ethylhexylphthalate, suppressed lymphocyte mitogenesis at the concentration of 10
−6–10
−5 M. We therefore suggest that estrogen may suppress lymphocyte mitogenesis through ER
α in B and T cells.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/S0378-4274(02)00203-5</identifier><identifier>PMID: 12119130</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; B cell ; Biological and medical sciences ; Bone Marrow Cells - drug effects ; Cell Division - drug effects ; Chemical and industrial products toxicology. Toxic occupational diseases ; Environmental Pollutants - toxicity ; Estradiol - toxicity ; Estrogen Receptor alpha ; Estrogen receptor α ; Estrogens - toxicity ; Estrogens, Non-Steroidal - toxicity ; Female ; Flow Cytometry ; Lymphocyte mitogenesis ; Lymphocytes - immunology ; Lymphocytes - metabolism ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Receptors, Estrogen - immunology ; Receptors, Estrogen - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - biosynthesis ; RNA - isolation & purification ; Spleen - immunology ; Spleen - metabolism ; Splenic lymphocyte ; Step-down PCR ; T cell ; Toxicology ; Various organic compounds</subject><ispartof>Toxicology letters, 2002-07, Vol.133 (2), p.221-229</ispartof><rights>2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-6ee9ca621e64eccdd75cb53aa0d21c980d34dee63f5a0363d8bdeafb3f4f4f6b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378427402002035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13763933$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12119130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakazaki, Humitoshi</creatorcontrib><creatorcontrib>Ueno, Hitoshi</creatorcontrib><creatorcontrib>Nakamuro, Katsuhiko</creatorcontrib><title>Estrogen receptor α in mouse splenic lymphocytes: possible involvement in immunity</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>To elucidate relevance of estrogens to immune responses, we investigated whether estrogen receptor
α (ER
α) exists in mouse splenic B cells and T cells and the effect of 17β-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ER
α was identified in both male and female mouse splenic cells using RT-PCR. Crude splenic cells were stained with anti-ER antibody, and the distribution of ER
α in the splenic B cells and part of the splenic T cells was confirmed by flow cytometry. 17β-Estradiol inhibited B cell mitogenesis at the concentration of 10
−8 M and T cell mitogenesis at the concentration of 10
−6 M. Some EDCs, diethylstilbestrol, bisphenol A,
p-nonylphenol and di-2-ethylhexylphthalate, suppressed lymphocyte mitogenesis at the concentration of 10
−6–10
−5 M. We therefore suggest that estrogen may suppress lymphocyte mitogenesis through ER
α in B and T cells.</description><subject>Animals</subject><subject>B cell</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Environmental Pollutants - toxicity</subject><subject>Estradiol - toxicity</subject><subject>Estrogen Receptor alpha</subject><subject>Estrogen receptor α</subject><subject>Estrogens - toxicity</subject><subject>Estrogens, Non-Steroidal - toxicity</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Lymphocyte mitogenesis</subject><subject>Lymphocytes - immunology</subject><subject>Lymphocytes - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Receptors, Estrogen - immunology</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - biosynthesis</subject><subject>RNA - isolation & purification</subject><subject>Spleen - immunology</subject><subject>Spleen - metabolism</subject><subject>Splenic lymphocyte</subject><subject>Step-down PCR</subject><subject>T cell</subject><subject>Toxicology</subject><subject>Various organic compounds</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqXwCaBsQLAI2LHjJGwQqspDqsSisLYcewJGeWEnlfJZ_AjfhPsQXaJZzObcmauD0CnB1wQTfrPANElDFiXsEkdXGEeYhvEeGpM0yUJKeLaPxn_ICB0594kx5ozHh2hEIkIyQvEYLWaus8071IEFBW3X2ODnOzB1UDW9g8C1JdRGBeVQtR-NGjpwt0HbOGfyEjy2bMolVFB3q4ipqr423XCMDgpZOjjZ7gl6e5i9Tp_C-cvj8_R-HiqWsC7kAJmSPCLAGSildRKrPKZSYh0RlaVYU6YBOC1iiSmnOs01yCKnBfPDczpBF5u7rW2-enCdqIxTUJayBt9ekJSlCcmYB-MNqKyvbqEQrTWVtIMgWKxsirVNsVIlcCTWNkXsc2fbB31egd6ltvo8cL4FpFOyLKyslXE7jiacZpR67m7DgdexNGCFUwZqBdp47Z3Qjfmnyi9ogZRk</recordid><startdate>20020721</startdate><enddate>20020721</enddate><creator>Sakazaki, Humitoshi</creator><creator>Ueno, Hitoshi</creator><creator>Nakamuro, Katsuhiko</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20020721</creationdate><title>Estrogen receptor α in mouse splenic lymphocytes: possible involvement in immunity</title><author>Sakazaki, Humitoshi ; Ueno, Hitoshi ; Nakamuro, Katsuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-6ee9ca621e64eccdd75cb53aa0d21c980d34dee63f5a0363d8bdeafb3f4f4f6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>B cell</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Environmental Pollutants - toxicity</topic><topic>Estradiol - toxicity</topic><topic>Estrogen Receptor alpha</topic><topic>Estrogen receptor α</topic><topic>Estrogens - toxicity</topic><topic>Estrogens, Non-Steroidal - toxicity</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Lymphocyte mitogenesis</topic><topic>Lymphocytes - immunology</topic><topic>Lymphocytes - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Receptors, Estrogen - immunology</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - biosynthesis</topic><topic>RNA - isolation & purification</topic><topic>Spleen - immunology</topic><topic>Spleen - metabolism</topic><topic>Splenic lymphocyte</topic><topic>Step-down PCR</topic><topic>T cell</topic><topic>Toxicology</topic><topic>Various organic compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakazaki, Humitoshi</creatorcontrib><creatorcontrib>Ueno, Hitoshi</creatorcontrib><creatorcontrib>Nakamuro, Katsuhiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakazaki, Humitoshi</au><au>Ueno, Hitoshi</au><au>Nakamuro, Katsuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estrogen receptor α in mouse splenic lymphocytes: possible involvement in immunity</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2002-07-21</date><risdate>2002</risdate><volume>133</volume><issue>2</issue><spage>221</spage><epage>229</epage><pages>221-229</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>To elucidate relevance of estrogens to immune responses, we investigated whether estrogen receptor
α (ER
α) exists in mouse splenic B cells and T cells and the effect of 17β-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ER
α was identified in both male and female mouse splenic cells using RT-PCR. Crude splenic cells were stained with anti-ER antibody, and the distribution of ER
α in the splenic B cells and part of the splenic T cells was confirmed by flow cytometry. 17β-Estradiol inhibited B cell mitogenesis at the concentration of 10
−8 M and T cell mitogenesis at the concentration of 10
−6 M. Some EDCs, diethylstilbestrol, bisphenol A,
p-nonylphenol and di-2-ethylhexylphthalate, suppressed lymphocyte mitogenesis at the concentration of 10
−6–10
−5 M. We therefore suggest that estrogen may suppress lymphocyte mitogenesis through ER
α in B and T cells.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>12119130</pmid><doi>10.1016/S0378-4274(02)00203-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Toxicology letters, 2002-07, Vol.133 (2), p.221-229 |
| issn | 0378-4274 1879-3169 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals B cell Biological and medical sciences Bone Marrow Cells - drug effects Cell Division - drug effects Chemical and industrial products toxicology. Toxic occupational diseases Environmental Pollutants - toxicity Estradiol - toxicity Estrogen Receptor alpha Estrogen receptor α Estrogens - toxicity Estrogens, Non-Steroidal - toxicity Female Flow Cytometry Lymphocyte mitogenesis Lymphocytes - immunology Lymphocytes - metabolism Male Medical sciences Mice Mice, Inbred BALB C Receptors, Estrogen - immunology Receptors, Estrogen - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA - biosynthesis RNA - isolation & purification Spleen - immunology Spleen - metabolism Splenic lymphocyte Step-down PCR T cell Toxicology Various organic compounds |
| title | Estrogen receptor α in mouse splenic lymphocytes: possible involvement in immunity |
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