Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell
[Display omitted] Lung cancer is recognized as the most prevalent type of cancer with high death rate. Ginsenoside Rg3 isolated from Traditional Chinese Medicine Panax Ginseng has significant anticancer effects on many tumors. In this study, the effects of ginsenoside Rg3 on cells viability, apoptos...
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| Veröffentlicht in: | Biomedicine & pharmacotherapy 2017-01, Vol.85, p.16-21 |
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| creator | Xie, Qipeng Wen, Huaikai Zhang, Qiong Zhou, Weihe Lin, Xiaoming Xie, Deyao Liu, Yu |
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Lung cancer is recognized as the most prevalent type of cancer with high death rate. Ginsenoside Rg3 isolated from Traditional Chinese Medicine Panax Ginseng has significant anticancer effects on many tumors. In this study, the effects of ginsenoside Rg3 on cells viability, apoptosis and PI3K/Akt signaling pathway in lung cancer cells were investigated in vitro and in vivo. In vitro, the viability of lung cancer cell lines A549，H23 was examined by CCK-8 kits; The proportion of cell apoptosis was measured by flow cytometry. The expression of p-PI3K/PI3K and p-Akt/Akt was evaluated with Western blot. In vivo, A549，H23 cells were subcutaneously injected into the nude mice. Histopathological analysis was stained with HE, and TUNEL assay was used to detect cell apoptosis. The results showed that Rg3 obviously inhibited cell viability, induced apoptosis and inhibited PI3K/Akt signalling pathway on A549, H23 cells in vitro and in vivo. Rg3 effectively inhibited the volume and weight of tumor in xenografts model, which may be related with inhibiting PI3K/Akt signaling pathways. |
| doi_str_mv | 10.1016/j.biopha.2016.11.096 |
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Lung cancer is recognized as the most prevalent type of cancer with high death rate. Ginsenoside Rg3 isolated from Traditional Chinese Medicine Panax Ginseng has significant anticancer effects on many tumors. In this study, the effects of ginsenoside Rg3 on cells viability, apoptosis and PI3K/Akt signaling pathway in lung cancer cells were investigated in vitro and in vivo. In vitro, the viability of lung cancer cell lines A549，H23 was examined by CCK-8 kits; The proportion of cell apoptosis was measured by flow cytometry. The expression of p-PI3K/PI3K and p-Akt/Akt was evaluated with Western blot. In vivo, A549，H23 cells were subcutaneously injected into the nude mice. Histopathological analysis was stained with HE, and TUNEL assay was used to detect cell apoptosis. The results showed that Rg3 obviously inhibited cell viability, induced apoptosis and inhibited PI3K/Akt signalling pathway on A549, H23 cells in vitro and in vivo. Rg3 effectively inhibited the volume and weight of tumor in xenografts model, which may be related with inhibiting PI3K/Akt signaling pathways.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/j.biopha.2016.11.096</identifier><identifier>PMID: 27930981</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Adenocarcinoma - drug therapy ; Animals ; Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Gene Expression Regulation - drug effects ; Ginsenoside Rg3 ; Ginsenosides - administration & dosage ; Ginsenosides - pharmacology ; Humans ; Internal Medicine ; Lung cancer ; Lung Neoplasms - drug therapy ; Medical Education ; Mice ; Mice, Nude ; Neoplasms, Experimental - drug therapy ; Phosphatidylinositol 3-Kinases - genetics ; Phosphatidylinositol 3-Kinases - metabolism ; Phytotherapy ; PI3K/Akt ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; Signal Transduction - drug effects</subject><ispartof>Biomedicine & pharmacotherapy, 2017-01, Vol.85, p.16-21</ispartof><rights>2016</rights><rights>Copyright © 2016. Published by Elsevier Masson SAS.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-7fc2d331f33f7a8ae6a0c59bda997d271fdab425efbf5b1dc74c5b63dd34775e3</citedby><cites>FETCH-LOGICAL-c417t-7fc2d331f33f7a8ae6a0c59bda997d271fdab425efbf5b1dc74c5b63dd34775e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0753332216311003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27930981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Qipeng</creatorcontrib><creatorcontrib>Wen, Huaikai</creatorcontrib><creatorcontrib>Zhang, Qiong</creatorcontrib><creatorcontrib>Zhou, Weihe</creatorcontrib><creatorcontrib>Lin, Xiaoming</creatorcontrib><creatorcontrib>Xie, Deyao</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><title>Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell</title><title>Biomedicine & pharmacotherapy</title><addtitle>Biomed Pharmacother</addtitle><description>[Display omitted]
Lung cancer is recognized as the most prevalent type of cancer with high death rate. Ginsenoside Rg3 isolated from Traditional Chinese Medicine Panax Ginseng has significant anticancer effects on many tumors. In this study, the effects of ginsenoside Rg3 on cells viability, apoptosis and PI3K/Akt signaling pathway in lung cancer cells were investigated in vitro and in vivo. In vitro, the viability of lung cancer cell lines A549，H23 was examined by CCK-8 kits; The proportion of cell apoptosis was measured by flow cytometry. The expression of p-PI3K/PI3K and p-Akt/Akt was evaluated with Western blot. In vivo, A549，H23 cells were subcutaneously injected into the nude mice. Histopathological analysis was stained with HE, and TUNEL assay was used to detect cell apoptosis. The results showed that Rg3 obviously inhibited cell viability, induced apoptosis and inhibited PI3K/Akt signalling pathway on A549, H23 cells in vitro and in vivo. Rg3 effectively inhibited the volume and weight of tumor in xenografts model, which may be related with inhibiting PI3K/Akt signaling pathways.</description><subject>Adenocarcinoma - drug therapy</subject><subject>Animals</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Ginsenoside Rg3</subject><subject>Ginsenosides - administration & dosage</subject><subject>Ginsenosides - pharmacology</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - drug therapy</subject><subject>Medical Education</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neoplasms, Experimental - drug therapy</subject><subject>Phosphatidylinositol 3-Kinases - genetics</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Phytotherapy</subject><subject>PI3K/Akt</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Signal Transduction - drug effects</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqBP0DISzYJrjiJkw3SaMSjNSOBeKwtxy53u0nsxk4a9d_jqAcWbFj5oVu3qs4l5CWwEhi0bw7l4MJxr8oqv0qAkvXtI7KBvmFFy5h4TDZMNLzgvKquyLOUDoyxpuXdU3JViZ6zvoMNmbZ-7wY3O7-jn7f8rri5m2lyO6_G9euo5v0vdaYuUedPYTyhyReq_OzmZQqRorWo50SDpTvnE_qQnEH6ZcdX3bhkD628xkg1juNz8sSqMeGLh_OafH__7tvtx-L-04ft7c19oWsQcyGsrgznYDm3QnUKW8V00w9G9b0wlQBr1FBXDdrBNgMYLWrdDC03htdCNMivyeuL7zGGnwumWU4urQMoj2FJErpadH0juMjS-iLVMaQU0cpjdJOKZwlMrqDlQV5AyxW0BJAZdC579dBhGSY0f4v-kM2CtxcB5j1PDqNM2mEmYVzMxKQJ7n8d_jXQORKn1fgDz5gOYYk5pLyLTJVk8usa9po1tByAMc5_A0vkpsw</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Xie, Qipeng</creator><creator>Wen, Huaikai</creator><creator>Zhang, Qiong</creator><creator>Zhou, Weihe</creator><creator>Lin, Xiaoming</creator><creator>Xie, Deyao</creator><creator>Liu, Yu</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell</title><author>Xie, Qipeng ; Wen, Huaikai ; Zhang, Qiong ; Zhou, Weihe ; Lin, Xiaoming ; Xie, Deyao ; Liu, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-7fc2d331f33f7a8ae6a0c59bda997d271fdab425efbf5b1dc74c5b63dd34775e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenocarcinoma - drug therapy</topic><topic>Animals</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Ginsenoside Rg3</topic><topic>Ginsenosides - administration & dosage</topic><topic>Ginsenosides - pharmacology</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - drug therapy</topic><topic>Medical Education</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Neoplasms, Experimental - drug therapy</topic><topic>Phosphatidylinositol 3-Kinases - genetics</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Phytotherapy</topic><topic>PI3K/Akt</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Signal Transduction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Qipeng</creatorcontrib><creatorcontrib>Wen, Huaikai</creatorcontrib><creatorcontrib>Zhang, Qiong</creatorcontrib><creatorcontrib>Zhou, Weihe</creatorcontrib><creatorcontrib>Lin, Xiaoming</creatorcontrib><creatorcontrib>Xie, Deyao</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine & pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Qipeng</au><au>Wen, Huaikai</au><au>Zhang, Qiong</au><au>Zhou, Weihe</au><au>Lin, Xiaoming</au><au>Xie, Deyao</au><au>Liu, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell</atitle><jtitle>Biomedicine & pharmacotherapy</jtitle><addtitle>Biomed Pharmacother</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>85</volume><spage>16</spage><epage>21</epage><pages>16-21</pages><issn>0753-3322</issn><eissn>1950-6007</eissn><abstract>[Display omitted]
Lung cancer is recognized as the most prevalent type of cancer with high death rate. Ginsenoside Rg3 isolated from Traditional Chinese Medicine Panax Ginseng has significant anticancer effects on many tumors. In this study, the effects of ginsenoside Rg3 on cells viability, apoptosis and PI3K/Akt signaling pathway in lung cancer cells were investigated in vitro and in vivo. In vitro, the viability of lung cancer cell lines A549，H23 was examined by CCK-8 kits; The proportion of cell apoptosis was measured by flow cytometry. The expression of p-PI3K/PI3K and p-Akt/Akt was evaluated with Western blot. In vivo, A549，H23 cells were subcutaneously injected into the nude mice. Histopathological analysis was stained with HE, and TUNEL assay was used to detect cell apoptosis. The results showed that Rg3 obviously inhibited cell viability, induced apoptosis and inhibited PI3K/Akt signalling pathway on A549, H23 cells in vitro and in vivo. Rg3 effectively inhibited the volume and weight of tumor in xenografts model, which may be related with inhibiting PI3K/Akt signaling pathways.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>27930981</pmid><doi>10.1016/j.biopha.2016.11.096</doi><tpages>6</tpages></addata></record> |
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| subjects | Adenocarcinoma - drug therapy Animals Antineoplastic Agents, Phytogenic - pharmacology Apoptosis Cell Line, Tumor Dose-Response Relationship, Drug Gene Expression Regulation - drug effects Ginsenoside Rg3 Ginsenosides - administration & dosage Ginsenosides - pharmacology Humans Internal Medicine Lung cancer Lung Neoplasms - drug therapy Medical Education Mice Mice, Nude Neoplasms, Experimental - drug therapy Phosphatidylinositol 3-Kinases - genetics Phosphatidylinositol 3-Kinases - metabolism Phytotherapy PI3K/Akt Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Signal Transduction - drug effects |
| title | Inhibiting PI3K-AKt signaling pathway is involved in antitumor effects of ginsenoside Rg3 in lung cancer cell |
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