Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm

Is it possible to improve clinical visualization of phospholipase C zeta (PLCζ) as a diagnostic marker of sperm oocyte activation capacity and male fertility? Poor PLCζ visualization efficacy using current protocols may be due to steric or conformational occlusion of native PLCζ, hindering antibody...

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Veröffentlicht in:	Molecular human reproduction 2017-01, Vol.23 (1), p.54-67
Hauptverfasser:	Kashir, Junaid, Buntwal, Luke, Nomikos, Michail, Calver, Brian L, Stamatiadis, Panagiotis, Ashley, Peter, Vassilakopoulou, Vyronia, Sanders, David, Knaggs, Paul, Livaniou, Evangelia, Bunkheila, Adnan, Swann, Karl, Lai, F Anthony
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosin - genetics Acrosin - immunology Animals Antibodies - chemistry Antibody Specificity Antigen-Antibody Complex - chemistry Biomarkers - metabolism Carrier Proteins - genetics Carrier Proteins - immunology Fluorescent Antibody Technique - standards Gene Expression Humans Infertility, Male - enzymology Infertility, Male - genetics Male Mice Oocytes - cytology Oocytes - physiology Phosphoinositide Phospholipase C - analysis Phosphoinositide Phospholipase C - chemistry Phosphoinositide Phospholipase C - genetics Phosphoinositide Phospholipase C - immunology Protein Binding Protein Conformation Seminal Plasma Proteins - genetics Seminal Plasma Proteins - immunology Sperm-Ovum Interactions - physiology Spermatozoa - enzymology Spermatozoa - pathology Swine Tissue Fixation - methods
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