New anonymous nuclear DNA markers for the pearl oyster Pinctada margaritifera and other Pinctada species

The black‐lipped oyster Pinctada margaritifera is highly exploited in French Polynesia where the pearl industry relies mostly on spat collection, and therefore on resources available in the wild. Little is known of these resources, and population genetic studies would be useful to improve management...

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Veröffentlicht in:Molecular ecology notes 2002-09, Vol.2 (3), p.220-222
Hauptverfasser: Arnaud-Haond, S., Boudry, P., Saulnier, D., Seaman, T., Vonau, V., Bonhomme, F., Goyard, E.
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container_end_page 222
container_issue 3
container_start_page 220
container_title Molecular ecology notes
container_volume 2
creator Arnaud-Haond, S.
Boudry, P.
Saulnier, D.
Seaman, T.
Vonau, V.
Bonhomme, F.
Goyard, E.
description The black‐lipped oyster Pinctada margaritifera is highly exploited in French Polynesia where the pearl industry relies mostly on spat collection, and therefore on resources available in the wild. Little is known of these resources, and population genetic studies would be useful to improve management. We used two methods, direct amplification of length polymorphism (DALP) and exon primed intron crossing (EPIC) to develop five new nuclear markers presenting length polymorphism. Although these markers remained anonymous after using blast on GenBank, they are codominant and follow Hardy–Weinberg equilibrium. Tests on related species or subspecies of Pinctada gave encouraging results.
doi_str_mv 10.1046/j.1471-8286.2002.00199.x
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subjects anonymous nuclear DNA
genetic resources
introns
length polymorphism
Marine
molecular markers
pearl oysters
title New anonymous nuclear DNA markers for the pearl oyster Pinctada margaritifera and other Pinctada species
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