New anonymous nuclear DNA markers for the pearl oyster Pinctada margaritifera and other Pinctada species
The black‐lipped oyster Pinctada margaritifera is highly exploited in French Polynesia where the pearl industry relies mostly on spat collection, and therefore on resources available in the wild. Little is known of these resources, and population genetic studies would be useful to improve management...
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Veröffentlicht in: | Molecular ecology notes 2002-09, Vol.2 (3), p.220-222 |
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container_title | Molecular ecology notes |
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creator | Arnaud-Haond, S. Boudry, P. Saulnier, D. Seaman, T. Vonau, V. Bonhomme, F. Goyard, E. |
description | The black‐lipped oyster Pinctada margaritifera is highly exploited in French Polynesia where the pearl industry relies mostly on spat collection, and therefore on resources available in the wild. Little is known of these resources, and population genetic studies would be useful to improve management. We used two methods, direct amplification of length polymorphism (DALP) and exon primed intron crossing (EPIC) to develop five new nuclear markers presenting length polymorphism. Although these markers remained anonymous after using blast on GenBank, they are codominant and follow Hardy–Weinberg equilibrium. Tests on related species or subspecies of Pinctada gave encouraging results. |
doi_str_mv | 10.1046/j.1471-8286.2002.00199.x |
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subjects | anonymous nuclear DNA genetic resources introns length polymorphism Marine molecular markers pearl oysters |
title | New anonymous nuclear DNA markers for the pearl oyster Pinctada margaritifera and other Pinctada species |
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