Pathogenic Role of Associated Adherent‐Invasive Escherichia coli in Crohn's Disease
Several lines of evidence suggest that adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies fr...
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| Veröffentlicht in: | Journal of cellular physiology 2017-10, Vol.232 (10), p.2860-2868 |
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| creator | Mazzarella, Giuseppe Perna, Angelica Marano, Angela Lucariello, Angela Rotondi Aufiero, Vera Sorrentino, Alida Melina, Raffaele Guerra, Germano Taccone, Fabio Silvio Iaquinto, Gaetano De Luca, Antonio |
| description | Several lines of evidence suggest that adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies from patients were set up to assess the effects of LF82 and O83:H1 on the expression of CEACAM6, LAMP1, HLA‐DR, ICAM1 by immunohistochemistry and of IL‐8, IFNʏ, and TNF‐α genes by RT‐PCR. Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT‐PCR, and DAPI staining, respectively. Epithelial and lamina propria mononuclear cells (LPMNC) expression of CEACAM6 and LAMP1 were higher in biopsies cultured in the presence of both O83:H1 and LF82 than in biopsies cultured with non‐pathogenic E. coli. Both AIEC strains induced increased expression of ICAM‐1 on blood vessels and HLA‐DR on LPMNC. We observed higher levels of TNF‐α, IFN‐γ, and IL‐8 transcripts in biopsies cultured with both AIEC strains than in those cultured with NP. Both LF82 and O83:H1, block the cell cycle into S phase, inducing DNA damage, and modulate the expression of DNMT1 and MGMT genes. Our data suggest that LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated. J. Cell. Physiol. 232: 2860–2868, 2017. © 2016 Wiley Periodicals, Inc.
Adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated. |
| doi_str_mv | 10.1002/jcp.25717 |
| format | Article |
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Adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.25717</identifier><identifier>PMID: 27925192</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Bacterial Adhesion ; Biopsy ; Blood vessels ; Caco-2 Cells ; Cell cycle ; Colon - immunology ; Colon - metabolism ; Colon - microbiology ; Colon - pathology ; Crohn Disease - immunology ; Crohn Disease - metabolism ; Crohn Disease - microbiology ; Crohn Disease - pathology ; Crohn's disease ; Cytokines ; Cytokines - immunology ; Cytokines - metabolism ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNMT1 protein ; E coli ; Escherichia coli - immunology ; Escherichia coli - isolation & purification ; Escherichia coli - pathogenicity ; Escherichia coli Infections - immunology ; Escherichia coli Infections - metabolism ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - pathology ; Female ; Flow cytometry ; Gene expression ; Genes ; Histocompatibility antigen HLA ; Host-Pathogen Interactions ; Humans ; Immunity, Mucosal ; Immunohistochemistry ; Inflammation ; Inflammation Mediators - immunology ; Inflammation Mediators - metabolism ; Inflammatory bowel diseases ; Intercellular adhesion molecule 1 ; Interleukin 8 ; Intestinal Mucosa - immunology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - microbiology ; Intestinal Mucosa - pathology ; Lamina propria ; Leukocytes (mononuclear) ; Male ; Middle Aged ; Mucosal immunity ; Patients ; Polymerase chain reaction ; S phase ; Staining ; Studies ; Tissue Culture Techniques ; Tumor necrosis factor-α ; Virulence ; Young Adult ; γ-Interferon</subject><ispartof>Journal of cellular physiology, 2017-10, Vol.232 (10), p.2860-2868</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4197-4b7b95ff2a9f49a91464bf4237bed129f0ed5b2bee498bda894ce0bf4d5a8df53</citedby><cites>FETCH-LOGICAL-c4197-4b7b95ff2a9f49a91464bf4237bed129f0ed5b2bee498bda894ce0bf4d5a8df53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.25717$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.25717$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27925192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazzarella, Giuseppe</creatorcontrib><creatorcontrib>Perna, Angelica</creatorcontrib><creatorcontrib>Marano, Angela</creatorcontrib><creatorcontrib>Lucariello, Angela</creatorcontrib><creatorcontrib>Rotondi Aufiero, Vera</creatorcontrib><creatorcontrib>Sorrentino, Alida</creatorcontrib><creatorcontrib>Melina, Raffaele</creatorcontrib><creatorcontrib>Guerra, Germano</creatorcontrib><creatorcontrib>Taccone, Fabio Silvio</creatorcontrib><creatorcontrib>Iaquinto, Gaetano</creatorcontrib><creatorcontrib>De Luca, Antonio</creatorcontrib><title>Pathogenic Role of Associated Adherent‐Invasive Escherichia coli in Crohn's Disease</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Several lines of evidence suggest that adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies from patients were set up to assess the effects of LF82 and O83:H1 on the expression of CEACAM6, LAMP1, HLA‐DR, ICAM1 by immunohistochemistry and of IL‐8, IFNʏ, and TNF‐α genes by RT‐PCR. Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT‐PCR, and DAPI staining, respectively. Epithelial and lamina propria mononuclear cells (LPMNC) expression of CEACAM6 and LAMP1 were higher in biopsies cultured in the presence of both O83:H1 and LF82 than in biopsies cultured with non‐pathogenic E. coli. Both AIEC strains induced increased expression of ICAM‐1 on blood vessels and HLA‐DR on LPMNC. We observed higher levels of TNF‐α, IFN‐γ, and IL‐8 transcripts in biopsies cultured with both AIEC strains than in those cultured with NP. Both LF82 and O83:H1, block the cell cycle into S phase, inducing DNA damage, and modulate the expression of DNMT1 and MGMT genes. Our data suggest that LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated. J. Cell. Physiol. 232: 2860–2868, 2017. © 2016 Wiley Periodicals, Inc.
Adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated.</description><subject>Adult</subject><subject>Aged</subject><subject>Bacterial Adhesion</subject><subject>Biopsy</subject><subject>Blood vessels</subject><subject>Caco-2 Cells</subject><subject>Cell cycle</subject><subject>Colon - immunology</subject><subject>Colon - metabolism</subject><subject>Colon - microbiology</subject><subject>Colon - pathology</subject><subject>Crohn Disease - immunology</subject><subject>Crohn Disease - metabolism</subject><subject>Crohn Disease - microbiology</subject><subject>Crohn Disease - pathology</subject><subject>Crohn's disease</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Cytokines - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNMT1 protein</subject><subject>E coli</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - immunology</subject><subject>Escherichia coli Infections - metabolism</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - pathology</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Histocompatibility antigen HLA</subject><subject>Host-Pathogen Interactions</subject><subject>Humans</subject><subject>Immunity, Mucosal</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Inflammation Mediators - immunology</subject><subject>Inflammation Mediators - metabolism</subject><subject>Inflammatory bowel diseases</subject><subject>Intercellular adhesion molecule 1</subject><subject>Interleukin 8</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Intestinal Mucosa - pathology</subject><subject>Lamina propria</subject><subject>Leukocytes (mononuclear)</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mucosal immunity</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>S phase</subject><subject>Staining</subject><subject>Studies</subject><subject>Tissue Culture Techniques</subject><subject>Tumor necrosis factor-α</subject><subject>Virulence</subject><subject>Young Adult</subject><subject>γ-Interferon</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEQgIMotlYPvoAEPKiHtkma_cmx1KoVwSL2HLLZiZuy3dTNbqU3H8Fn9Enc2upB8DQw8_ExfAidUtKjhLD-XC97LIhotIfalIioy8OA7aN2c6NdEXDaQkfezwkhQgwGh6jFIsECKlgbzaaqytwLFFbjJ5cDdgYPvXfaqgpSPEwzKKGoPt8_JsVKebsCPPa6WVqdWYW1yy22BR6VLisuPL62HpSHY3RgVO7hZDc7aHYzfh7ddR8ebyej4UNXc7p5M4kSERjDlDBcKEF5yBPD2SBKIKVMGAJpkLAEgIs4SVUsuAbSEGmg4tQEgw663HqXpXutwVdyYb2GPFcFuNpLGvMwYowK0qDnf9C5q8ui-U5SQVkYUs42wqstpUvnfQlGLku7UOVaUiI3rWXTWn63btiznbFOFpD-kj9xG6C_Bd5sDuv_TfJ-NN0qvwAcmojl</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Mazzarella, Giuseppe</creator><creator>Perna, Angelica</creator><creator>Marano, Angela</creator><creator>Lucariello, Angela</creator><creator>Rotondi Aufiero, Vera</creator><creator>Sorrentino, Alida</creator><creator>Melina, Raffaele</creator><creator>Guerra, Germano</creator><creator>Taccone, Fabio Silvio</creator><creator>Iaquinto, Gaetano</creator><creator>De Luca, Antonio</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201710</creationdate><title>Pathogenic Role of Associated Adherent‐Invasive Escherichia coli in Crohn's Disease</title><author>Mazzarella, Giuseppe ; Perna, Angelica ; Marano, Angela ; Lucariello, Angela ; Rotondi Aufiero, Vera ; Sorrentino, Alida ; Melina, Raffaele ; Guerra, Germano ; Taccone, Fabio Silvio ; Iaquinto, Gaetano ; De Luca, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4197-4b7b95ff2a9f49a91464bf4237bed129f0ed5b2bee498bda894ce0bf4d5a8df53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bacterial Adhesion</topic><topic>Biopsy</topic><topic>Blood vessels</topic><topic>Caco-2 Cells</topic><topic>Cell cycle</topic><topic>Colon - immunology</topic><topic>Colon - metabolism</topic><topic>Colon - microbiology</topic><topic>Colon - pathology</topic><topic>Crohn Disease - immunology</topic><topic>Crohn Disease - metabolism</topic><topic>Crohn Disease - microbiology</topic><topic>Crohn Disease - pathology</topic><topic>Crohn's disease</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Cytokines - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNMT1 protein</topic><topic>E coli</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - immunology</topic><topic>Escherichia coli Infections - metabolism</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - pathology</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Histocompatibility antigen HLA</topic><topic>Host-Pathogen Interactions</topic><topic>Humans</topic><topic>Immunity, Mucosal</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Inflammation Mediators - immunology</topic><topic>Inflammation Mediators - metabolism</topic><topic>Inflammatory bowel diseases</topic><topic>Intercellular adhesion molecule 1</topic><topic>Interleukin 8</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Intestinal Mucosa - pathology</topic><topic>Lamina propria</topic><topic>Leukocytes (mononuclear)</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mucosal immunity</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>S phase</topic><topic>Staining</topic><topic>Studies</topic><topic>Tissue Culture Techniques</topic><topic>Tumor necrosis factor-α</topic><topic>Virulence</topic><topic>Young Adult</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazzarella, Giuseppe</creatorcontrib><creatorcontrib>Perna, Angelica</creatorcontrib><creatorcontrib>Marano, Angela</creatorcontrib><creatorcontrib>Lucariello, Angela</creatorcontrib><creatorcontrib>Rotondi Aufiero, Vera</creatorcontrib><creatorcontrib>Sorrentino, Alida</creatorcontrib><creatorcontrib>Melina, Raffaele</creatorcontrib><creatorcontrib>Guerra, Germano</creatorcontrib><creatorcontrib>Taccone, Fabio Silvio</creatorcontrib><creatorcontrib>Iaquinto, Gaetano</creatorcontrib><creatorcontrib>De Luca, Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazzarella, Giuseppe</au><au>Perna, Angelica</au><au>Marano, Angela</au><au>Lucariello, Angela</au><au>Rotondi Aufiero, Vera</au><au>Sorrentino, Alida</au><au>Melina, Raffaele</au><au>Guerra, Germano</au><au>Taccone, Fabio Silvio</au><au>Iaquinto, Gaetano</au><au>De Luca, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogenic Role of Associated Adherent‐Invasive Escherichia coli in Crohn's Disease</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2017-10</date><risdate>2017</risdate><volume>232</volume><issue>10</issue><spage>2860</spage><epage>2868</epage><pages>2860-2868</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Several lines of evidence suggest that adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. Organ cultures of colonic biopsies from patients were set up to assess the effects of LF82 and O83:H1 on the expression of CEACAM6, LAMP1, HLA‐DR, ICAM1 by immunohistochemistry and of IL‐8, IFNʏ, and TNF‐α genes by RT‐PCR. Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT‐PCR, and DAPI staining, respectively. Epithelial and lamina propria mononuclear cells (LPMNC) expression of CEACAM6 and LAMP1 were higher in biopsies cultured in the presence of both O83:H1 and LF82 than in biopsies cultured with non‐pathogenic E. coli. Both AIEC strains induced increased expression of ICAM‐1 on blood vessels and HLA‐DR on LPMNC. We observed higher levels of TNF‐α, IFN‐γ, and IL‐8 transcripts in biopsies cultured with both AIEC strains than in those cultured with NP. Both LF82 and O83:H1, block the cell cycle into S phase, inducing DNA damage, and modulate the expression of DNMT1 and MGMT genes. Our data suggest that LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated. J. Cell. Physiol. 232: 2860–2868, 2017. © 2016 Wiley Periodicals, Inc.
Adherent‐invasive Escherichia coli (AIEC) strains play an important role in Crohn's disease (CD). The objective of this study was to investigate the pathogenic role of two AIEC strains, LF82 and O83:H1, in CD patients. LF82 and 083:H1 strains of E. coli are able to increase in CD colonic biopsies the expression of all the pro‐inflammatory cytokines and all the mucosal immune markers investigated.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27925192</pmid><doi>10.1002/jcp.25717</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged Bacterial Adhesion Biopsy Blood vessels Caco-2 Cells Cell cycle Colon - immunology Colon - metabolism Colon - microbiology Colon - pathology Crohn Disease - immunology Crohn Disease - metabolism Crohn Disease - microbiology Crohn Disease - pathology Crohn's disease Cytokines Cytokines - immunology Cytokines - metabolism Deoxyribonucleic acid DNA DNA damage DNMT1 protein E coli Escherichia coli - immunology Escherichia coli - isolation & purification Escherichia coli - pathogenicity Escherichia coli Infections - immunology Escherichia coli Infections - metabolism Escherichia coli Infections - microbiology Escherichia coli Infections - pathology Female Flow cytometry Gene expression Genes Histocompatibility antigen HLA Host-Pathogen Interactions Humans Immunity, Mucosal Immunohistochemistry Inflammation Inflammation Mediators - immunology Inflammation Mediators - metabolism Inflammatory bowel diseases Intercellular adhesion molecule 1 Interleukin 8 Intestinal Mucosa - immunology Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Lamina propria Leukocytes (mononuclear) Male Middle Aged Mucosal immunity Patients Polymerase chain reaction S phase Staining Studies Tissue Culture Techniques Tumor necrosis factor-α Virulence Young Adult γ-Interferon |
| title | Pathogenic Role of Associated Adherent‐Invasive Escherichia coli in Crohn's Disease |
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