Creating BHb-imprinted magnetic nanoparticles with multiple binding sites
A kind of protein imprinted over magnetic Fe 3 O 4 @Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe 3 O 4 @Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce...
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| Veröffentlicht in: | Analyst (London) 2017-01, Vol.142 (2), p.32-39 |
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| container_title | Analyst (London) |
| container_volume | 142 |
| creator | Li, Yanxia Chen, Yiting Huang, Lu Lou, BenYong Chen, Guonan |
| description | A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe
3
O
4
@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g
−1
) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL
−1
within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.
A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. |
| doi_str_mv | 10.1039/c6an02121b |
| format | Article |
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3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe
3
O
4
@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g
−1
) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL
−1
within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.
A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c6an02121b</identifier><identifier>PMID: 27924985</identifier><language>eng</language><publisher>England</publisher><subject>Adsorption ; Binding sites ; Carboxyl group ; Nanomaterials ; Nanoparticles ; Proteins ; Strategy ; Synthesis</subject><ispartof>Analyst (London), 2017-01, Vol.142 (2), p.32-39</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-5deb076d323cef34ac10a53090377eea642aa24f6736f59233d0d502e9b7293d3</citedby><cites>FETCH-LOGICAL-c368t-5deb076d323cef34ac10a53090377eea642aa24f6736f59233d0d502e9b7293d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27924985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yanxia</creatorcontrib><creatorcontrib>Chen, Yiting</creatorcontrib><creatorcontrib>Huang, Lu</creatorcontrib><creatorcontrib>Lou, BenYong</creatorcontrib><creatorcontrib>Chen, Guonan</creatorcontrib><title>Creating BHb-imprinted magnetic nanoparticles with multiple binding sites</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe
3
O
4
@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g
−1
) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL
−1
within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.
A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated.</description><subject>Adsorption</subject><subject>Binding sites</subject><subject>Carboxyl group</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Proteins</subject><subject>Strategy</subject><subject>Synthesis</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqN0T1PwzAQBmALgWj5WNhBYUNIAX_Edjy2EdBKFSwwR058KUaJG-xEiH9PSkvZEJPvdM_d8BqhM4JvCGbqthTaYUooKfbQmDCRxJzTdB-NMcYspoInI3QUwtvQEszxIRpRqWiiUj5G88yD7qxbRtNZEdum9dZ1YKJGLx10toycdqtW-6GsIUQftnuNmr7ubFtDVFhn1qvBdhBO0EGl6wCn2_cYvdzfPWezePH0MM8mi7hkIu1ibqDAUhhGWQkVS3RJsOYMK8ykBNAioVrTpBKSiYorypjBhmMKqpBUMcOO0dXmbutX7z2ELm9sKKGutYNVH3KSSqVSRUT6D5oISYkicqDXG1r6VQgeqnxIotH-Myc4X6ecZ2Ly-J3ydMAX27t90YDZ0Z9YB3C5AT6Uu-nvN-WtqQZz_pdhXx_Bi8c</recordid><startdate>20170116</startdate><enddate>20170116</enddate><creator>Li, Yanxia</creator><creator>Chen, Yiting</creator><creator>Huang, Lu</creator><creator>Lou, BenYong</creator><creator>Chen, Guonan</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20170116</creationdate><title>Creating BHb-imprinted magnetic nanoparticles with multiple binding sites</title><author>Li, Yanxia ; Chen, Yiting ; Huang, Lu ; Lou, BenYong ; Chen, Guonan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-5deb076d323cef34ac10a53090377eea642aa24f6736f59233d0d502e9b7293d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adsorption</topic><topic>Binding sites</topic><topic>Carboxyl group</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Proteins</topic><topic>Strategy</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yanxia</creatorcontrib><creatorcontrib>Chen, Yiting</creatorcontrib><creatorcontrib>Huang, Lu</creatorcontrib><creatorcontrib>Lou, BenYong</creatorcontrib><creatorcontrib>Chen, Guonan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yanxia</au><au>Chen, Yiting</au><au>Huang, Lu</au><au>Lou, BenYong</au><au>Chen, Guonan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Creating BHb-imprinted magnetic nanoparticles with multiple binding sites</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2017-01-16</date><risdate>2017</risdate><volume>142</volume><issue>2</issue><spage>32</spage><epage>39</epage><pages>32-39</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe
3
O
4
@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups. The MMIPs exhibited an excellent imprinting effect and adsorption capacity (89.65± 0.38 mg g
−1
) toward the template protein. The results show that the MMIPs reached saturated adsorption at 0.5 mg mL
−1
within 90 min. The synthesized MMIPs are suitable for the removal and enrichment of the template protein in proteomics. The strategy of multiple binding sites paves the way for the preparation of functional nanomaterials in molecular imprinting techniques.
A kind of protein imprinted over magnetic Fe
3
O
4
@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated.</abstract><cop>England</cop><pmid>27924985</pmid><doi>10.1039/c6an02121b</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analyst (London), 2017-01, Vol.142 (2), p.32-39 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1846721917 |
| source | Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
| subjects | Adsorption Binding sites Carboxyl group Nanomaterials Nanoparticles Proteins Strategy Synthesis |
| title | Creating BHb-imprinted magnetic nanoparticles with multiple binding sites |
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