Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis, angiogenesis and survivability of cultured buffalo luteal cells
Contents The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the...
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| Veröffentlicht in: | Reproduction in domestic animals 2016-12, Vol.51 (6), p.855-869 |
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| creator | Mishra, SR Parmar, MS Yadav, VP Reshma, R Bharati, J Bharti, MK Paul, A Chouhan, VS Taru Sharma, G Singh, G Sarkar, M |
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The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p |
| doi_str_mv | 10.1111/rda.12739 |
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The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P4 secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P4 secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12739</identifier><identifier>PMID: 27569719</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Angiogenesis ; Angiopoietins - genetics ; Angiopoietins - metabolism ; Animal reproduction ; Animals ; bcl-Associated Death Protein - genetics ; bcl-Associated Death Protein - metabolism ; Blotting, Western ; Buffalo ; Buffaloes - physiology ; Caspase 3 - genetics ; Caspase 3 - metabolism ; Cell Survival - physiology ; Cells, Cultured ; Corpus Luteum - metabolism ; Estrous Cycle - physiology ; Female ; Gene Expression Regulation - physiology ; Immunohistochemistry ; Kinases ; Luteal Cells - physiology ; Neovascularization, Physiologic - physiology ; Phosphatidylinositols - genetics ; Phosphatidylinositols - metabolism ; Progesterone ; Progesterone - genetics ; Progesterone - metabolism ; Protein Transport ; Proteins ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; Receptors, TIE - genetics ; Receptors, TIE - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; von Willebrand Factor - genetics ; von Willebrand Factor - metabolism</subject><ispartof>Reproduction in domestic animals, 2016-12, Vol.51 (6), p.855-869</ispartof><rights>2016 Blackwell Verlag GmbH</rights><rights>2016 Blackwell Verlag GmbH.</rights><rights>Copyright © 2016 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4249-2e64be5f573505cfaddfeb8c5d56a9a98d19edabd73932a0d03887696e91f8ef3</citedby><cites>FETCH-LOGICAL-c4249-2e64be5f573505cfaddfeb8c5d56a9a98d19edabd73932a0d03887696e91f8ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12739$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12739$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27569719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mishra, SR</creatorcontrib><creatorcontrib>Parmar, MS</creatorcontrib><creatorcontrib>Yadav, VP</creatorcontrib><creatorcontrib>Reshma, R</creatorcontrib><creatorcontrib>Bharati, J</creatorcontrib><creatorcontrib>Bharti, MK</creatorcontrib><creatorcontrib>Paul, A</creatorcontrib><creatorcontrib>Chouhan, VS</creatorcontrib><creatorcontrib>Taru Sharma, G</creatorcontrib><creatorcontrib>Singh, G</creatorcontrib><creatorcontrib>Sarkar, M</creatorcontrib><title>Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis, angiogenesis and survivability of cultured buffalo luteal cells</title><title>Reproduction in domestic animals</title><addtitle>Reprod Dom Anim</addtitle><description>Contents
The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P4 secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P4 secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL.</description><subject>Angiogenesis</subject><subject>Angiopoietins - genetics</subject><subject>Angiopoietins - metabolism</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>bcl-Associated Death Protein - genetics</subject><subject>bcl-Associated Death Protein - metabolism</subject><subject>Blotting, Western</subject><subject>Buffalo</subject><subject>Buffaloes - physiology</subject><subject>Caspase 3 - genetics</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Corpus Luteum - metabolism</subject><subject>Estrous Cycle - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Luteal Cells - physiology</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Phosphatidylinositols - genetics</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Progesterone</subject><subject>Progesterone - genetics</subject><subject>Progesterone - metabolism</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Receptors, TIE - genetics</subject><subject>Receptors, TIE - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>von Willebrand Factor - genetics</subject><subject>von Willebrand Factor - metabolism</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt9uFSEQxjdGY4_VC1_AbOKNJm4LywLLZVNrqznWxGj0jrDLcEJllyMstesD-xxy_rQXTUzkBvjym29mYIriOUZHOK_joNURrjkRD4oFboioECX4YbFAgrCKcdYeFE9ivEII05bzx8VBzSkTHItF8efsZh0gRuvHUo26dL5Xzv5W00bwJmsr69fewmTH0qjBurnMp96HdYqlSxOkodQp2HFVamsMBBinMk5qBXET7yFOwWe0n3sH2xSD18mpyYe5DD5r97LksDEbQPBW-xWMEG18syP2t61LTOHaXqvOOjvNG48-uSkF0GWXjFHOb4tTruzBufi0eJS1CM_2-2Hx9d3Zl9OLavnp_P3pybLqm7oRVQ2s6YAayglFtDdKawNd21NNmRJKtBoL0KrT-a1JrZBGpG05EwwENi0Ycli82vmug_-Zcu9ysHFTgRohv4LEbcMazFsh_getKWkoYRl9eQ-98imMuZFMESZQjTDO1Osd1QcfYwAj18EOKswSI7mZE5nnRG7nJLMv9o6pG0DfkbeDkYHjHfDLOpj_7SQ_vz25tax2ETZ_3s1dhAo_JOOEU_nt8lxSctF8-Pj9Ui7JX1Nw3gI</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Mishra, SR</creator><creator>Parmar, MS</creator><creator>Yadav, VP</creator><creator>Reshma, R</creator><creator>Bharati, J</creator><creator>Bharti, MK</creator><creator>Paul, A</creator><creator>Chouhan, VS</creator><creator>Taru Sharma, G</creator><creator>Singh, G</creator><creator>Sarkar, M</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis, angiogenesis and survivability of cultured buffalo luteal cells</title><author>Mishra, SR ; Parmar, MS ; Yadav, VP ; Reshma, R ; Bharati, J ; Bharti, MK ; Paul, A ; Chouhan, VS ; Taru Sharma, G ; Singh, G ; Sarkar, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4249-2e64be5f573505cfaddfeb8c5d56a9a98d19edabd73932a0d03887696e91f8ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Angiogenesis</topic><topic>Angiopoietins - genetics</topic><topic>Angiopoietins - metabolism</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>bcl-Associated Death Protein - genetics</topic><topic>bcl-Associated Death Protein - metabolism</topic><topic>Blotting, Western</topic><topic>Buffalo</topic><topic>Buffaloes - physiology</topic><topic>Caspase 3 - genetics</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Corpus Luteum - metabolism</topic><topic>Estrous Cycle - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Luteal Cells - physiology</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Phosphatidylinositols - genetics</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Progesterone</topic><topic>Progesterone - genetics</topic><topic>Progesterone - metabolism</topic><topic>Protein Transport</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Receptors, TIE - genetics</topic><topic>Receptors, TIE - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>von Willebrand Factor - genetics</topic><topic>von Willebrand Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mishra, SR</creatorcontrib><creatorcontrib>Parmar, MS</creatorcontrib><creatorcontrib>Yadav, VP</creatorcontrib><creatorcontrib>Reshma, R</creatorcontrib><creatorcontrib>Bharati, J</creatorcontrib><creatorcontrib>Bharti, MK</creatorcontrib><creatorcontrib>Paul, A</creatorcontrib><creatorcontrib>Chouhan, VS</creatorcontrib><creatorcontrib>Taru Sharma, G</creatorcontrib><creatorcontrib>Singh, G</creatorcontrib><creatorcontrib>Sarkar, M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mishra, SR</au><au>Parmar, MS</au><au>Yadav, VP</au><au>Reshma, R</au><au>Bharati, J</au><au>Bharti, MK</au><au>Paul, A</au><au>Chouhan, VS</au><au>Taru Sharma, G</au><au>Singh, G</au><au>Sarkar, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis, angiogenesis and survivability of cultured buffalo luteal cells</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Dom Anim</addtitle><date>2016-12</date><risdate>2016</risdate><volume>51</volume><issue>6</issue><spage>855</spage><epage>869</epage><pages>855-869</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P4 secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P4 secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>27569719</pmid><doi>10.1111/rda.12739</doi><tpages>15</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2016-12, Vol.51 (6), p.855-869 |
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| subjects | Angiogenesis Angiopoietins - genetics Angiopoietins - metabolism Animal reproduction Animals bcl-Associated Death Protein - genetics bcl-Associated Death Protein - metabolism Blotting, Western Buffalo Buffaloes - physiology Caspase 3 - genetics Caspase 3 - metabolism Cell Survival - physiology Cells, Cultured Corpus Luteum - metabolism Estrous Cycle - physiology Female Gene Expression Regulation - physiology Immunohistochemistry Kinases Luteal Cells - physiology Neovascularization, Physiologic - physiology Phosphatidylinositols - genetics Phosphatidylinositols - metabolism Progesterone Progesterone - genetics Progesterone - metabolism Protein Transport Proteins Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Receptors, TIE - genetics Receptors, TIE - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism von Willebrand Factor - genetics von Willebrand Factor - metabolism |
| title | Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis, angiogenesis and survivability of cultured buffalo luteal cells |
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