Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide: e108367

The sapB gene, encoding Bacillus pumilus CBS protease, and seven mutated genes (sapB-L31I, sapB-T33S, sapB-N99Y, sapB-L31I/T33S, sapB-L31I/N99Y, sapB-T33S/N99Y, and sapB-L31I/T33S/N99Y) were overexpressed in protease-deficient Bacillus subtilis DB430 and purified to homogeneity. SAPB-N99Y and rSAPB...

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Veröffentlicht in:	PloS one 2014-09, Vol.9 (9)
Hauptverfasser:	Jaouadi, Nadia Zarai, Jaouadi, Bassem, Hlima, Hajer Ben, Rekik, Hatem, Belhoul, Mouna, Hmidi, Maher, Aicha, Houda SlimeneBen, Hila, Chiraz Gorgi, Toumi, Abdessatar, Aghajari, Nushin
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus pumilus Bacillus subtilis
Online-Zugang:	Volltext
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