A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti
A proteomic atlas of a model legume and its rhizobial symbiont provides a resource for understanding symbiotic nitrogen fixation. Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex sy...
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| Veröffentlicht in: | Nature biotechnology 2016-11, Vol.34 (11), p.1198-1205 |
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| creator | Marx, Harald Minogue, Catherine E Jayaraman, Dhileepkumar Richards, Alicia L Kwiecien, Nicholas W Siahpirani, Alireza F Rajasekar, Shanmugam Maeda, Junko Garcia, Kevin Del Valle-Echevarria, Angel R Volkening, Jeremy D Westphall, Michael S Roy, Sushmita Sussman, Michael R Ané, Jean-Michel Coon, Joshua J |
| description | A proteomic atlas of a model legume and its rhizobial symbiont provides a resource for understanding symbiotic nitrogen fixation.
Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex symbiotic association between legumes and nitrogen-fixing soil bacteria called rhizobia culminates in the development of root nodules, where rhizobia fix atmospheric nitrogen and transfer it to their plant host. Here we describe a quantitative proteomic atlas of the model legume
Medicago truncatula
and its rhizobial symbiont
Sinorhizobium meliloti
, which includes more than 23,000 proteins, 20,000 phosphorylation sites, and 700 lysine acetylation sites. Our analysis provides insight into mechanisms regulating symbiosis. We identify a calmodulin-binding protein as a key regulator in the host and assign putative roles and targets to host factors (bioactive peptides) that control gene expression in the symbiont. Further mining of this proteomic resource may enable engineering of crops and their microbial partners to increase agricultural productivity and sustainability. |
| doi_str_mv | 10.1038/nbt.3681 |
| format | Article |
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Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex symbiotic association between legumes and nitrogen-fixing soil bacteria called rhizobia culminates in the development of root nodules, where rhizobia fix atmospheric nitrogen and transfer it to their plant host. Here we describe a quantitative proteomic atlas of the model legume
Medicago truncatula
and its rhizobial symbiont
Sinorhizobium meliloti
, which includes more than 23,000 proteins, 20,000 phosphorylation sites, and 700 lysine acetylation sites. Our analysis provides insight into mechanisms regulating symbiosis. We identify a calmodulin-binding protein as a key regulator in the host and assign putative roles and targets to host factors (bioactive peptides) that control gene expression in the symbiont. Further mining of this proteomic resource may enable engineering of crops and their microbial partners to increase agricultural productivity and sustainability.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt.3681</identifier><identifier>PMID: 27748755</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/449/2676/2678 ; 631/61/475 ; 82/58 ; 82/80 ; 82/81 ; Agricultural production ; Agriculture ; Alfalfa ; Bacteria ; Bacterial Proteins - metabolism ; Bioinformatics ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Databases, Protein ; Farming systems ; Fertilizers ; Legumes ; Life Sciences ; Medicago truncatula ; Medicago truncatula - metabolism ; Medicago truncatula - microbiology ; Methods ; Nitrogen fixation ; Nitrogen Fixation - physiology ; Nitrogen-fixing microorganisms ; Peptides ; Physiological aspects ; Plant Proteins - metabolism ; Proteome - metabolism ; Proteomics ; resource ; Sinorhizobium meliloti ; Sinorhizobium meliloti - physiology ; Soil microorganisms ; Sustainability ; Symbiosis ; Symbiosis - physiology</subject><ispartof>Nature biotechnology, 2016-11, Vol.34 (11), p.1198-1205</ispartof><rights>Springer Nature America, Inc. 2016</rights><rights>COPYRIGHT 2016 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-6b55a6649a3b41224437db72a54fb8b821656926c6b6e5a5d27ceb6f15c5cf753</citedby><cites>FETCH-LOGICAL-c650t-6b55a6649a3b41224437db72a54fb8b821656926c6b6e5a5d27ceb6f15c5cf753</cites><orcidid>0000-0002-7804-4084</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27748755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marx, Harald</creatorcontrib><creatorcontrib>Minogue, Catherine E</creatorcontrib><creatorcontrib>Jayaraman, Dhileepkumar</creatorcontrib><creatorcontrib>Richards, Alicia L</creatorcontrib><creatorcontrib>Kwiecien, Nicholas W</creatorcontrib><creatorcontrib>Siahpirani, Alireza F</creatorcontrib><creatorcontrib>Rajasekar, Shanmugam</creatorcontrib><creatorcontrib>Maeda, Junko</creatorcontrib><creatorcontrib>Garcia, Kevin</creatorcontrib><creatorcontrib>Del Valle-Echevarria, Angel R</creatorcontrib><creatorcontrib>Volkening, Jeremy D</creatorcontrib><creatorcontrib>Westphall, Michael S</creatorcontrib><creatorcontrib>Roy, Sushmita</creatorcontrib><creatorcontrib>Sussman, Michael R</creatorcontrib><creatorcontrib>Ané, Jean-Michel</creatorcontrib><creatorcontrib>Coon, Joshua J</creatorcontrib><title>A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>A proteomic atlas of a model legume and its rhizobial symbiont provides a resource for understanding symbiotic nitrogen fixation.
Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex symbiotic association between legumes and nitrogen-fixing soil bacteria called rhizobia culminates in the development of root nodules, where rhizobia fix atmospheric nitrogen and transfer it to their plant host. Here we describe a quantitative proteomic atlas of the model legume
Medicago truncatula
and its rhizobial symbiont
Sinorhizobium meliloti
, which includes more than 23,000 proteins, 20,000 phosphorylation sites, and 700 lysine acetylation sites. Our analysis provides insight into mechanisms regulating symbiosis. We identify a calmodulin-binding protein as a key regulator in the host and assign putative roles and targets to host factors (bioactive peptides) that control gene expression in the symbiont. Further mining of this proteomic resource may enable engineering of crops and their microbial partners to increase agricultural productivity and sustainability.</description><subject>631/449/2676/2678</subject><subject>631/61/475</subject><subject>82/58</subject><subject>82/80</subject><subject>82/81</subject><subject>Agricultural production</subject><subject>Agriculture</subject><subject>Alfalfa</subject><subject>Bacteria</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bioinformatics</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Databases, Protein</subject><subject>Farming systems</subject><subject>Fertilizers</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Medicago truncatula</subject><subject>Medicago truncatula - metabolism</subject><subject>Medicago truncatula - microbiology</subject><subject>Methods</subject><subject>Nitrogen 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Sushmita</au><au>Sussman, Michael R</au><au>Ané, Jean-Michel</au><au>Coon, Joshua J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>34</volume><issue>11</issue><spage>1198</spage><epage>1205</epage><pages>1198-1205</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><abstract>A proteomic atlas of a model legume and its rhizobial symbiont provides a resource for understanding symbiotic nitrogen fixation.
Legumes are essential components of agricultural systems because they enrich the soil in nitrogen and require little environmentally deleterious fertilizers. A complex symbiotic association between legumes and nitrogen-fixing soil bacteria called rhizobia culminates in the development of root nodules, where rhizobia fix atmospheric nitrogen and transfer it to their plant host. Here we describe a quantitative proteomic atlas of the model legume
Medicago truncatula
and its rhizobial symbiont
Sinorhizobium meliloti
, which includes more than 23,000 proteins, 20,000 phosphorylation sites, and 700 lysine acetylation sites. Our analysis provides insight into mechanisms regulating symbiosis. We identify a calmodulin-binding protein as a key regulator in the host and assign putative roles and targets to host factors (bioactive peptides) that control gene expression in the symbiont. Further mining of this proteomic resource may enable engineering of crops and their microbial partners to increase agricultural productivity and sustainability.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>27748755</pmid><doi>10.1038/nbt.3681</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7804-4084</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | 631/449/2676/2678 631/61/475 82/58 82/80 82/81 Agricultural production Agriculture Alfalfa Bacteria Bacterial Proteins - metabolism Bioinformatics Biomedical Engineering/Biotechnology Biomedicine Biotechnology Databases, Protein Farming systems Fertilizers Legumes Life Sciences Medicago truncatula Medicago truncatula - metabolism Medicago truncatula - microbiology Methods Nitrogen fixation Nitrogen Fixation - physiology Nitrogen-fixing microorganisms Peptides Physiological aspects Plant Proteins - metabolism Proteome - metabolism Proteomics resource Sinorhizobium meliloti Sinorhizobium meliloti - physiology Soil microorganisms Sustainability Symbiosis Symbiosis - physiology |
| title | A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti |
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