Multi-target spectrally resolved fluorescence lifetime imaging microscopy

Spectrally resolved FLIM with three excitation wavelengths and detection on 32 channels combined with advanced pattern matching allows for simultaneous detection and discrimination of fluorophores with nearly identical emission spectra, enabling highly multiplexed imaging. We introduce a pattern-mat...

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Veröffentlicht in:	Nature methods 2016-03, Vol.13 (3), p.257-262
Hauptverfasser:	Niehörster, Thomas, Löschberger, Anna, Gregor, Ingo, Krämer, Benedikt, Rahn, Hans-Jürgen, Patting, Matthias, Koberling, Felix, Enderlein, Jörg, Sauer, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	14 14/19 14/34 14/35 14/63 631/57/2282 631/80/2373 Algorithms Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Cellular biology Emissions Environmental factors Fluorescence Fluorescence microscopy Humans Image Enhancement - methods Image Interpretation, Computer-Assisted - methods Life Sciences Methods Mice Microscopy Microscopy, Confocal - methods Microscopy, Fluorescence, Multiphoton - methods Molecules Pattern Recognition, Automated - methods Proteomics Reproducibility of Results Sensitivity and Specificity Wavelengths
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