Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro
The small airway-on-a-chip allows the recapitulation of human lung pathophysiology in vitro and analysis of responses to drugs. Here we describe the development of a human lung 'small airway-on-a-chip' containing a differentiated, mucociliary bronchiolar epithelium and an underlying microv...
Gespeichert in:
| Veröffentlicht in: | Nature methods 2016-02, Vol.13 (2), p.151-157 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 157 |
|---|---|
| container_issue | 2 |
| container_start_page | 151 |
| container_title | Nature methods |
| container_volume | 13 |
| creator | Benam, Kambez H Villenave, Remi Lucchesi, Carolina Varone, Antonio Hubeau, Cedric Lee, Hyun-Hee Alves, Stephen E Salmon, Michael Ferrante, Thomas C Weaver, James C Bahinski, Anthony Hamilton, Geraldine A Ingber, Donald E |
| description | The small airway-on-a-chip allows the recapitulation of human lung pathophysiology
in vitro
and analysis of responses to drugs.
Here we describe the development of a human lung 'small airway-on-a-chip' containing a differentiated, mucociliary bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which allows for analysis of organ-level lung pathophysiology
in vitro
. Exposure of the epithelium to interleukin-13 (IL-13) reconstituted the goblet cell hyperplasia, cytokine hypersecretion and decreased ciliary function of asthmatics. Small airway chips lined with epithelial cells from individuals with chronic obstructive pulmonary disease recapitulated features of the disease such as selective cytokine hypersecretion, increased neutrophil recruitment and clinical exacerbation by exposure to viral and bacterial infections. With this robust
in vitro
method for modeling human lung inflammatory disorders, it is possible to detect synergistic effects of lung endothelium and epithelium on cytokine secretion, identify new biomarkers of disease exacerbation and measure responses to anti-inflammatory compounds that inhibit cytokine-induced recruitment of circulating neutrophils under flow. |
| doi_str_mv | 10.1038/nmeth.3697 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1846400056</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A441882868</galeid><sourcerecordid>A441882868</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-5943cd869b4d1c121e826c772d34a4ae0b6044800710ca4157afea3cd1bd38173</originalsourceid><addsrcrecordid>eNqFkdtrFDEYxQex2Fp98Q-QgC9FmTXJ5DaPpXiDQh_UV8M3mcxuSi5rMqPsf99st_UOkoeE5HcO-c5pmmcErwju1OsY7LxZdaKXD5oTwplqJcH84f0Z9-S4eVzKNcZdxyh_1BxTIVRPBT1pvnwM4D0Cl7_Drk2xhdZs3BbZCIO3BUEEvyuuoDShzRIgIr_ENXJx8hACzC7FyoxozMsaZVu2KZYqcxF9c3NOT5qjCXyxT-_20-bz2zefLt63l1fvPlycX7aGczm3vGedGZXoBzYSQyixigojJR07BgwsHgRmTGFcBzPACJcwWagSMoydIrI7bc4Ovtucvi62zDq4Yqz3EG1aiiaKCYYx5uL_qBSEiZocreiLP9DrtOSayC0lCVeSkp_UGrzVNZk0ZzB7U33OGFGKKqEqtfoHVddogzMp2snV-98ELw8Ck1Mp2U56m12AvNME633v-rZ3ve-9ws_vfroMwY4_0PuiK_DqAJT6FNc2_zLK33Y3z361PA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1767158721</pqid></control><display><type>article</type><title>Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro</title><source>MEDLINE</source><source>Nature Journals Online</source><source>SpringerLink Journals - AutoHoldings</source><creator>Benam, Kambez H ; Villenave, Remi ; Lucchesi, Carolina ; Varone, Antonio ; Hubeau, Cedric ; Lee, Hyun-Hee ; Alves, Stephen E ; Salmon, Michael ; Ferrante, Thomas C ; Weaver, James C ; Bahinski, Anthony ; Hamilton, Geraldine A ; Ingber, Donald E</creator><creatorcontrib>Benam, Kambez H ; Villenave, Remi ; Lucchesi, Carolina ; Varone, Antonio ; Hubeau, Cedric ; Lee, Hyun-Hee ; Alves, Stephen E ; Salmon, Michael ; Ferrante, Thomas C ; Weaver, James C ; Bahinski, Anthony ; Hamilton, Geraldine A ; Ingber, Donald E</creatorcontrib><description>The small airway-on-a-chip allows the recapitulation of human lung pathophysiology
in vitro
and analysis of responses to drugs.
Here we describe the development of a human lung 'small airway-on-a-chip' containing a differentiated, mucociliary bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which allows for analysis of organ-level lung pathophysiology
in vitro
. Exposure of the epithelium to interleukin-13 (IL-13) reconstituted the goblet cell hyperplasia, cytokine hypersecretion and decreased ciliary function of asthmatics. Small airway chips lined with epithelial cells from individuals with chronic obstructive pulmonary disease recapitulated features of the disease such as selective cytokine hypersecretion, increased neutrophil recruitment and clinical exacerbation by exposure to viral and bacterial infections. With this robust
in vitro
method for modeling human lung inflammatory disorders, it is possible to detect synergistic effects of lung endothelium and epithelium on cytokine secretion, identify new biomarkers of disease exacerbation and measure responses to anti-inflammatory compounds that inhibit cytokine-induced recruitment of circulating neutrophils under flow.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth.3697</identifier><identifier>PMID: 26689262</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>13 ; 13/1 ; 13/106 ; 13/107 ; 13/21 ; 13/31 ; 13/51 ; 13/62 ; 14 ; 14/19 ; 14/28 ; 14/34 ; 14/63 ; 631/1647/277 ; 631/250/127 ; 631/443/1784 ; 692/308/575 ; Analysis ; Asthma ; Bacterial diseases ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical Engineering/Biotechnology ; Care and treatment ; Chronic obstructive pulmonary disease ; Drug metabolism ; Drugs ; Epithelium - drug effects ; Humans ; Immune response ; Inflammation ; Inflammation - metabolism ; Inflammation - pathology ; Interleukin-13 - pharmacology ; Lab-On-A-Chip Devices ; Life Sciences ; Lung Diseases - drug therapy ; Lung Diseases - metabolism ; Lungs ; Observations ; Pathology ; Physiology ; Proteomics ; Regulation ; Synergistic effect ; Tissue Culture Techniques</subject><ispartof>Nature methods, 2016-02, Vol.13 (2), p.151-157</ispartof><rights>Springer Nature America, Inc. 2015</rights><rights>COPYRIGHT 2016 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-5943cd869b4d1c121e826c772d34a4ae0b6044800710ca4157afea3cd1bd38173</citedby><cites>FETCH-LOGICAL-c557t-5943cd869b4d1c121e826c772d34a4ae0b6044800710ca4157afea3cd1bd38173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth.3697$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth.3697$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26689262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benam, Kambez H</creatorcontrib><creatorcontrib>Villenave, Remi</creatorcontrib><creatorcontrib>Lucchesi, Carolina</creatorcontrib><creatorcontrib>Varone, Antonio</creatorcontrib><creatorcontrib>Hubeau, Cedric</creatorcontrib><creatorcontrib>Lee, Hyun-Hee</creatorcontrib><creatorcontrib>Alves, Stephen E</creatorcontrib><creatorcontrib>Salmon, Michael</creatorcontrib><creatorcontrib>Ferrante, Thomas C</creatorcontrib><creatorcontrib>Weaver, James C</creatorcontrib><creatorcontrib>Bahinski, Anthony</creatorcontrib><creatorcontrib>Hamilton, Geraldine A</creatorcontrib><creatorcontrib>Ingber, Donald E</creatorcontrib><title>Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>The small airway-on-a-chip allows the recapitulation of human lung pathophysiology
in vitro
and analysis of responses to drugs.
Here we describe the development of a human lung 'small airway-on-a-chip' containing a differentiated, mucociliary bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which allows for analysis of organ-level lung pathophysiology
in vitro
. Exposure of the epithelium to interleukin-13 (IL-13) reconstituted the goblet cell hyperplasia, cytokine hypersecretion and decreased ciliary function of asthmatics. Small airway chips lined with epithelial cells from individuals with chronic obstructive pulmonary disease recapitulated features of the disease such as selective cytokine hypersecretion, increased neutrophil recruitment and clinical exacerbation by exposure to viral and bacterial infections. With this robust
in vitro
method for modeling human lung inflammatory disorders, it is possible to detect synergistic effects of lung endothelium and epithelium on cytokine secretion, identify new biomarkers of disease exacerbation and measure responses to anti-inflammatory compounds that inhibit cytokine-induced recruitment of circulating neutrophils under flow.</description><subject>13</subject><subject>13/1</subject><subject>13/106</subject><subject>13/107</subject><subject>13/21</subject><subject>13/31</subject><subject>13/51</subject><subject>13/62</subject><subject>14</subject><subject>14/19</subject><subject>14/28</subject><subject>14/34</subject><subject>14/63</subject><subject>631/1647/277</subject><subject>631/250/127</subject><subject>631/443/1784</subject><subject>692/308/575</subject><subject>Analysis</subject><subject>Asthma</subject><subject>Bacterial diseases</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Care and treatment</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Drug metabolism</subject><subject>Drugs</subject><subject>Epithelium - drug effects</subject><subject>Humans</subject><subject>Immune response</subject><subject>Inflammation</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - pathology</subject><subject>Interleukin-13 - pharmacology</subject><subject>Lab-On-A-Chip Devices</subject><subject>Life Sciences</subject><subject>Lung Diseases - drug therapy</subject><subject>Lung Diseases - metabolism</subject><subject>Lungs</subject><subject>Observations</subject><subject>Pathology</subject><subject>Physiology</subject><subject>Proteomics</subject><subject>Regulation</subject><subject>Synergistic effect</subject><subject>Tissue Culture Techniques</subject><issn>1548-7091</issn><issn>1548-7105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkdtrFDEYxQex2Fp98Q-QgC9FmTXJ5DaPpXiDQh_UV8M3mcxuSi5rMqPsf99st_UOkoeE5HcO-c5pmmcErwju1OsY7LxZdaKXD5oTwplqJcH84f0Z9-S4eVzKNcZdxyh_1BxTIVRPBT1pvnwM4D0Cl7_Drk2xhdZs3BbZCIO3BUEEvyuuoDShzRIgIr_ENXJx8hACzC7FyoxozMsaZVu2KZYqcxF9c3NOT5qjCXyxT-_20-bz2zefLt63l1fvPlycX7aGczm3vGedGZXoBzYSQyixigojJR07BgwsHgRmTGFcBzPACJcwWagSMoydIrI7bc4Ovtucvi62zDq4Yqz3EG1aiiaKCYYx5uL_qBSEiZocreiLP9DrtOSayC0lCVeSkp_UGrzVNZk0ZzB7U33OGFGKKqEqtfoHVddogzMp2snV-98ELw8Ck1Mp2U56m12AvNME633v-rZ3ve-9ws_vfroMwY4_0PuiK_DqAJT6FNc2_zLK33Y3z361PA</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Benam, Kambez H</creator><creator>Villenave, Remi</creator><creator>Lucchesi, Carolina</creator><creator>Varone, Antonio</creator><creator>Hubeau, Cedric</creator><creator>Lee, Hyun-Hee</creator><creator>Alves, Stephen E</creator><creator>Salmon, Michael</creator><creator>Ferrante, Thomas C</creator><creator>Weaver, James C</creator><creator>Bahinski, Anthony</creator><creator>Hamilton, Geraldine A</creator><creator>Ingber, Donald E</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7SS</scope><scope>7TK</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro</title><author>Benam, Kambez H ; Villenave, Remi ; Lucchesi, Carolina ; Varone, Antonio ; Hubeau, Cedric ; Lee, Hyun-Hee ; Alves, Stephen E ; Salmon, Michael ; Ferrante, Thomas C ; Weaver, James C ; Bahinski, Anthony ; Hamilton, Geraldine A ; Ingber, Donald E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-5943cd869b4d1c121e826c772d34a4ae0b6044800710ca4157afea3cd1bd38173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13</topic><topic>13/1</topic><topic>13/106</topic><topic>13/107</topic><topic>13/21</topic><topic>13/31</topic><topic>13/51</topic><topic>13/62</topic><topic>14</topic><topic>14/19</topic><topic>14/28</topic><topic>14/34</topic><topic>14/63</topic><topic>631/1647/277</topic><topic>631/250/127</topic><topic>631/443/1784</topic><topic>692/308/575</topic><topic>Analysis</topic><topic>Asthma</topic><topic>Bacterial diseases</topic><topic>Bioinformatics</topic><topic>Biological Microscopy</topic><topic>Biological Techniques</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Care and treatment</topic><topic>Chronic obstructive pulmonary disease</topic><topic>Drug metabolism</topic><topic>Drugs</topic><topic>Epithelium - drug effects</topic><topic>Humans</topic><topic>Immune response</topic><topic>Inflammation</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - pathology</topic><topic>Interleukin-13 - pharmacology</topic><topic>Lab-On-A-Chip Devices</topic><topic>Life Sciences</topic><topic>Lung Diseases - drug therapy</topic><topic>Lung Diseases - metabolism</topic><topic>Lungs</topic><topic>Observations</topic><topic>Pathology</topic><topic>Physiology</topic><topic>Proteomics</topic><topic>Regulation</topic><topic>Synergistic effect</topic><topic>Tissue Culture Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benam, Kambez H</creatorcontrib><creatorcontrib>Villenave, Remi</creatorcontrib><creatorcontrib>Lucchesi, Carolina</creatorcontrib><creatorcontrib>Varone, Antonio</creatorcontrib><creatorcontrib>Hubeau, Cedric</creatorcontrib><creatorcontrib>Lee, Hyun-Hee</creatorcontrib><creatorcontrib>Alves, Stephen E</creatorcontrib><creatorcontrib>Salmon, Michael</creatorcontrib><creatorcontrib>Ferrante, Thomas C</creatorcontrib><creatorcontrib>Weaver, James C</creatorcontrib><creatorcontrib>Bahinski, Anthony</creatorcontrib><creatorcontrib>Hamilton, Geraldine A</creatorcontrib><creatorcontrib>Ingber, Donald E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benam, Kambez H</au><au>Villenave, Remi</au><au>Lucchesi, Carolina</au><au>Varone, Antonio</au><au>Hubeau, Cedric</au><au>Lee, Hyun-Hee</au><au>Alves, Stephen E</au><au>Salmon, Michael</au><au>Ferrante, Thomas C</au><au>Weaver, James C</au><au>Bahinski, Anthony</au><au>Hamilton, Geraldine A</au><au>Ingber, Donald E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>13</volume><issue>2</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>The small airway-on-a-chip allows the recapitulation of human lung pathophysiology
in vitro
and analysis of responses to drugs.
Here we describe the development of a human lung 'small airway-on-a-chip' containing a differentiated, mucociliary bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which allows for analysis of organ-level lung pathophysiology
in vitro
. Exposure of the epithelium to interleukin-13 (IL-13) reconstituted the goblet cell hyperplasia, cytokine hypersecretion and decreased ciliary function of asthmatics. Small airway chips lined with epithelial cells from individuals with chronic obstructive pulmonary disease recapitulated features of the disease such as selective cytokine hypersecretion, increased neutrophil recruitment and clinical exacerbation by exposure to viral and bacterial infections. With this robust
in vitro
method for modeling human lung inflammatory disorders, it is possible to detect synergistic effects of lung endothelium and epithelium on cytokine secretion, identify new biomarkers of disease exacerbation and measure responses to anti-inflammatory compounds that inhibit cytokine-induced recruitment of circulating neutrophils under flow.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>26689262</pmid><doi>10.1038/nmeth.3697</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1548-7091 |
| ispartof | Nature methods, 2016-02, Vol.13 (2), p.151-157 |
| issn | 1548-7091 1548-7105 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1846400056 |
| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | 13 13/1 13/106 13/107 13/21 13/31 13/51 13/62 14 14/19 14/28 14/34 14/63 631/1647/277 631/250/127 631/443/1784 692/308/575 Analysis Asthma Bacterial diseases Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Care and treatment Chronic obstructive pulmonary disease Drug metabolism Drugs Epithelium - drug effects Humans Immune response Inflammation Inflammation - metabolism Inflammation - pathology Interleukin-13 - pharmacology Lab-On-A-Chip Devices Life Sciences Lung Diseases - drug therapy Lung Diseases - metabolism Lungs Observations Pathology Physiology Proteomics Regulation Synergistic effect Tissue Culture Techniques |
| title | Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T19%3A50%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Small%20airway-on-a-chip%20enables%20analysis%20of%20human%20lung%20inflammation%20and%20drug%20responses%20in%20vitro&rft.jtitle=Nature%20methods&rft.au=Benam,%20Kambez%20H&rft.date=2016-02-01&rft.volume=13&rft.issue=2&rft.spage=151&rft.epage=157&rft.pages=151-157&rft.issn=1548-7091&rft.eissn=1548-7105&rft_id=info:doi/10.1038/nmeth.3697&rft_dat=%3Cgale_proqu%3EA441882868%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1767158721&rft_id=info:pmid/26689262&rft_galeid=A441882868&rfr_iscdi=true |