Antimicrobial action of an intracanal medication trial using Aloe vera

Abstract Objective: This study aimed to test the calcium hydroxide associated with vehicles Aloe vera, chlorhexidine digluconate (2%) and saline, in inhibiting bacterial growth on Mueller-Hinton agar plates. Methods: The species Staphiloccocus aureus, Streptococcus pyogenes, Escherichia coli and Enterecoccus faecalis were isolated and inoculated in 3 mL of BHI (Brain Heart Infusion). Holes (5 mm diameter) were made in the plates and filled with the test materials. After incubation, readings were taken with a hand lens and a caliper with 0.1 mm accuracy to determine the diameter of the inhibition zone, after 24 and 48 hours. Each experiment was repeated six times, and the average values were obtained. Results: Chlorhexidine digluconate without calcium hydroxide resulted in better inhibition of bacterial growth, followed by the hydroxide pastes evaluated. E. coli strains were the most resistant to the tested compounds, followed by S. pyogenes and S. aureus. There was no statistically significant interaction between the variables. Conclusion: Chlorhexidine digluconate (2%) alone showed the best antimicrobial effectiveness. Aloe vera is a promising vehicle for the calcium hydroxide but more studies should be conducted on herbal medicines in dentistry.