Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds
3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and os...
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| Veröffentlicht in: | Doklady. Biochemistry and biophysics 2016-09, Vol.470 (1), p.368-370 |
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| container_title | Doklady. Biochemistry and biophysics |
| container_volume | 470 |
| creator | Goncharenko, A. V. Malyuchenko, N. V. Moisenovich, A. M. Kotlyarova, M. S. Arkhipova, A. Yu Kon’kov, A. S. Agapov, I. I. Molochkov, A. V. Moisenovich, M. M. Kirpichnikov, M. P. |
| description | 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and osteogenic calcium phosphate component of the microcarrier. |
| doi_str_mv | 10.1134/S1607672916050197 |
| format | Article |
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V. ; Malyuchenko, N. V. ; Moisenovich, A. M. ; Kotlyarova, M. S. ; Arkhipova, A. Yu ; Kon’kov, A. S. ; Agapov, I. I. ; Molochkov, A. V. ; Moisenovich, M. M. ; Kirpichnikov, M. P.</creator><creatorcontrib>Goncharenko, A. V. ; Malyuchenko, N. V. ; Moisenovich, A. M. ; Kotlyarova, M. S. ; Arkhipova, A. Yu ; Kon’kov, A. S. ; Agapov, I. I. ; Molochkov, A. V. ; Moisenovich, M. M. ; Kirpichnikov, M. P.</creatorcontrib><description>3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. 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Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and osteogenic calcium phosphate component of the microcarrier.</description><subject>Actin Cytoskeleton - metabolism</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Bone Regeneration</subject><subject>Bone Substitutes</subject><subject>Bones</subject><subject>Calcium Phosphates - metabolism</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Fibroins</subject><subject>Gelatin</subject><subject>Gene expression</subject><subject>Glass</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Molecular Biology</subject><subject>Morphology</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis - physiology</subject><subject>Phosphatase</subject><subject>Porosity</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds</subject><issn>1607-6729</issn><issn>1608-3091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc1u1TAQhS1ERUvhAdggS2zYBPzveIluoSAVdVFYR05i96Z14uBxEOVteFOcey8IgZC6mpHnm3PGOgg9o-QVpVy8vqKKaKWZKVUSavQDdFLauuLE0Ie7Xlfr_Bg9BrghhBHG5SN0zHRNNaHqBP3YbO107QAPEx5jmrcxxOs7HD22XS5vfgh2dFMGbKceu29zcgBDnHZEuLVhmBwuWzBvbbbgsM2Yn-FuCXn4avOB_HheKY4jZBfbYCFXYbh1uHMhAC7EWERSkfru-mLYpliMobPex9DDE3TkbQD39FBP0ed3bz9t3lcXl-cfNm8uqk4QmSstmRdCtVa2Xrne85YJYrnTtRasVbXl0iivRN1KXRsmBaE9kbrXtBM994yfopd73TnFL4uD3IwDrCfaycUFGloLxY003NwD5ZoUix364i_0Ji5pKh9ZKcVVzcxK0T3VpQiQnG_mNIw23TWUNGvUzT9Rl53nB-WlHV3_e-NXtgVgewDKqGSc_rD-r-pP9Y-zbg</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Goncharenko, A. 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| identifier | ISSN: 1607-6729 |
| ispartof | Doklady. Biochemistry and biophysics, 2016-09, Vol.470 (1), p.368-370 |
| issn | 1607-6729 1608-3091 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Actin Cytoskeleton - metabolism Alkaline Phosphatase - metabolism Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Bone Regeneration Bone Substitutes Bones Calcium Phosphates - metabolism Cell Adhesion Cell Culture Techniques Cell Line Fibroins Gelatin Gene expression Glass Humans Life Sciences Molecular Biology Morphology Osteoblasts - cytology Osteoblasts - metabolism Osteogenesis - physiology Phosphatase Porosity Tissue engineering Tissue Scaffolds |
| title | Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds |
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