Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study
[Display omitted] Schematic diagram of the bacitracin-modified Ti in vivo study. The multiple biofunctions of Ti could be realized by immobilization of bacitracin on the Ti surface. •Bacitracin-functionlized Ti enables prophylaxis of implant infection in vivo.•The bacitracin-immobilized Ti exhibits...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2017-02, Vol.150, p.183-191 |
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| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Nie, Bin’en Ao, Haiyong Long, Teng Zhou, Jianliang Tang, Tingting Yue, Bing |
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Schematic diagram of the bacitracin-modified Ti in vivo study. The multiple biofunctions of Ti could be realized by immobilization of bacitracin on the Ti surface.
•Bacitracin-functionlized Ti enables prophylaxis of implant infection in vivo.•The bacitracin-immobilized Ti exhibits osteoinductivity in a rat model.•Biofunctionalized Ti could be realized via immobilization of bacitracin.
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo. A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo, fifteen SD rats were divided into three groups (N=5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects. |
| doi_str_mv | 10.1016/j.colsurfb.2016.11.034 |
| format | Article |
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Schematic diagram of the bacitracin-modified Ti in vivo study. The multiple biofunctions of Ti could be realized by immobilization of bacitracin on the Ti surface.
•Bacitracin-functionlized Ti enables prophylaxis of implant infection in vivo.•The bacitracin-immobilized Ti exhibits osteoinductivity in a rat model.•Biofunctionalized Ti could be realized via immobilization of bacitracin.
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo. A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo, fifteen SD rats were divided into three groups (N=5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2016.11.034</identifier><identifier>PMID: 27914255</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Bacitracin ; Bacitracin - chemistry ; Bone and Bones - metabolism ; Bone Substitutes ; Coated Materials, Biocompatible - pharmacology ; Dental Implants ; Female ; Femur - surgery ; Immobilization ; Immobilized Proteins - chemistry ; Infections ; Osseoinductivity ; Osseointegration - physiology ; Osteomyelitis - drug therapy ; Rats ; Rats, Sprague-Dawley ; Staphylococcus aureus ; Surface Properties ; Titanium - chemistry ; Titanium alloy ; X-Ray Microtomography ; X-Rays</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2017-02, Vol.150, p.183-191</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-75a561f752b9859d2d0456da8271b25c4af54eac84491384694b088df2ae8c1e3</citedby><cites>FETCH-LOGICAL-c471t-75a561f752b9859d2d0456da8271b25c4af54eac84491384694b088df2ae8c1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.colsurfb.2016.11.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27914255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nie, Bin’en</creatorcontrib><creatorcontrib>Ao, Haiyong</creatorcontrib><creatorcontrib>Long, Teng</creatorcontrib><creatorcontrib>Zhou, Jianliang</creatorcontrib><creatorcontrib>Tang, Tingting</creatorcontrib><creatorcontrib>Yue, Bing</creatorcontrib><title>Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>[Display omitted]
Schematic diagram of the bacitracin-modified Ti in vivo study. The multiple biofunctions of Ti could be realized by immobilization of bacitracin on the Ti surface.
•Bacitracin-functionlized Ti enables prophylaxis of implant infection in vivo.•The bacitracin-immobilized Ti exhibits osteoinductivity in a rat model.•Biofunctionalized Ti could be realized via immobilization of bacitracin.
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo. A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo, fifteen SD rats were divided into three groups (N=5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Bacitracin</subject><subject>Bacitracin - chemistry</subject><subject>Bone and Bones - metabolism</subject><subject>Bone Substitutes</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Dental Implants</subject><subject>Female</subject><subject>Femur - surgery</subject><subject>Immobilization</subject><subject>Immobilized Proteins - chemistry</subject><subject>Infections</subject><subject>Osseoinductivity</subject><subject>Osseointegration - physiology</subject><subject>Osteomyelitis - drug therapy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Staphylococcus aureus</subject><subject>Surface Properties</subject><subject>Titanium - chemistry</subject><subject>Titanium alloy</subject><subject>X-Ray Microtomography</subject><subject>X-Rays</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EotvCX6h85JLUdvyRcKKqClSqxAXOluMPmFViL3YSsfx6vGzLlctYIz3vzPhB6JqSlhIqb_atTVNZcxhbVvuW0pZ0_AXa0V51De-keol2ZGCqUUqKC3RZyp4QwjhVr9EFUwPlTIgd2h7mOY0wwW-I3_FoLCy5lohTxAssJsI645AyPuR0-HGczC8oOAUMMXi7QIoFm-j-EjBXZjuNSWXxCaJbK7HBcnyPb2NN4A22hMuyuuMb9CqYqfi3T-8V-vbx_uvd5-bxy6eHu9vHxnJFl0YJIyQNSrBx6MXgmCNcSGd6pujIhOUmCO6N7TkfaNdzOfCR9L0LzPjeUt9doXfnufW0n6svi56hWD9NJvq0Fk1rhjApOa-oPKM2p1KyD_qQYTb5qCnRJ-d6r5-d65NzTamuzmvw-mnHOs7e_Ys9S67AhzPg60838FkXCz5a7yBXidol-N-OPzW9mMA</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Nie, Bin’en</creator><creator>Ao, Haiyong</creator><creator>Long, Teng</creator><creator>Zhou, Jianliang</creator><creator>Tang, Tingting</creator><creator>Yue, Bing</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study</title><author>Nie, Bin’en ; Ao, Haiyong ; Long, Teng ; Zhou, Jianliang ; Tang, Tingting ; Yue, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-75a561f752b9859d2d0456da8271b25c4af54eac84491384694b088df2ae8c1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Bacitracin</topic><topic>Bacitracin - chemistry</topic><topic>Bone and Bones - metabolism</topic><topic>Bone Substitutes</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Dental Implants</topic><topic>Female</topic><topic>Femur - surgery</topic><topic>Immobilization</topic><topic>Immobilized Proteins - chemistry</topic><topic>Infections</topic><topic>Osseoinductivity</topic><topic>Osseointegration - physiology</topic><topic>Osteomyelitis - drug therapy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Staphylococcus aureus</topic><topic>Surface Properties</topic><topic>Titanium - chemistry</topic><topic>Titanium alloy</topic><topic>X-Ray Microtomography</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nie, Bin’en</creatorcontrib><creatorcontrib>Ao, Haiyong</creatorcontrib><creatorcontrib>Long, Teng</creatorcontrib><creatorcontrib>Zhou, Jianliang</creatorcontrib><creatorcontrib>Tang, Tingting</creatorcontrib><creatorcontrib>Yue, Bing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nie, Bin’en</au><au>Ao, Haiyong</au><au>Long, Teng</au><au>Zhou, Jianliang</au><au>Tang, Tingting</au><au>Yue, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>150</volume><spage>183</spage><epage>191</epage><pages>183-191</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>[Display omitted]
Schematic diagram of the bacitracin-modified Ti in vivo study. The multiple biofunctions of Ti could be realized by immobilization of bacitracin on the Ti surface.
•Bacitracin-functionlized Ti enables prophylaxis of implant infection in vivo.•The bacitracin-immobilized Ti exhibits osteoinductivity in a rat model.•Biofunctionalized Ti could be realized via immobilization of bacitracin.
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo. A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo, fifteen SD rats were divided into three groups (N=5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27914255</pmid><doi>10.1016/j.colsurfb.2016.11.034</doi><tpages>9</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2017-02, Vol.150, p.183-191 |
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| subjects | Animals Anti-Bacterial Agents - chemistry Bacitracin Bacitracin - chemistry Bone and Bones - metabolism Bone Substitutes Coated Materials, Biocompatible - pharmacology Dental Implants Female Femur - surgery Immobilization Immobilized Proteins - chemistry Infections Osseoinductivity Osseointegration - physiology Osteomyelitis - drug therapy Rats Rats, Sprague-Dawley Staphylococcus aureus Surface Properties Titanium - chemistry Titanium alloy X-Ray Microtomography X-Rays |
| title | Immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: An in vivo study |
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