Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity
The synthesis of 2-nitro-5-[(hexanoyl)-amino]-benzoic acid and 2-nitro-5-[(octanoyl)-amino]-benzoic acid as chromogenic substrates for the determination of aliphatic penicillin acylase activity is described. During enzymatic hydrolysis, the released chromophore, 2-nitro-5-amino-benzoic acid, was det...
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| Veröffentlicht in: | Biotechnology letters 2002-07, Vol.24 (13), p.1045-1048 |
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| container_title | Biotechnology letters |
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| creator | ARROYO, Miguel TORRES-GUZMAN, Raquel TORRES-BACETE, Jesus DE LA MATA, Isabel CASTILLON, Maria Pilar ACEBAL, Carmen |
| description | The synthesis of 2-nitro-5-[(hexanoyl)-amino]-benzoic acid and 2-nitro-5-[(octanoyl)-amino]-benzoic acid as chromogenic substrates for the determination of aliphatic penicillin acylase activity is described. During enzymatic hydrolysis, the released chromophore, 2-nitro-5-amino-benzoic acid, was detected at 405 nm. Penicillin acylase from Streptomyces lavendulae had an appreciable activity towards these substrates, which can then be used to detect penicillin acylases able to cleave hexanoyl and octanoyl residues off synthetic amides as well as penicillin dihydroF and penicillin K, their natural analogues.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/A:1016096111453 |
| format | Article |
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| source | SpringerNature Journals |
| subjects | Amides Biological and medical sciences Biotechnology Enzyme engineering Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Penicillin |
| title | Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity |
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