Global DNA Methylation Detection System Using MBD-Fused Luciferase Based on Bioluminescence Resonance Energy Transfer Assay

DNA methylation plays an important role in the regulation of gene expression. In normal cells, transposable elements that constitute approximately 45% of the human genome are highly methylated to silence their expression. In cancer cells, transposable elements are hypomethylated; therefore, global D...

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Veröffentlicht in:	Analytical chemistry (Washington) 2016-09, Vol.88 (18), p.9264-9268
Hauptverfasser:	Yoshida, Wataru, Baba, Yuji, Karube, Isao
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Bioluminescence Biomarkers Biosensing Techniques - methods Cancer CpG Islands Deoxyribonucleic acid DNA Methylation DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Energy Transfer Gene expression Genomics HeLa Cells Humans Luciferases - chemistry Luciferases - metabolism Luminescent Measurements - methods Methylation Models, Molecular Neoplasms - diagnosis Neoplasms - genetics Protein Domains Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism
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