Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection
In this paper, we reported the preparation of carboxyl functionalized quantum dots (QDs)-embedded silica nanoparticles by combining layer-by-layer (LbL) self-assembly technique and a multi-layer protection method. Preformed SiO 2 nanoparticles synthesized via the Stöber method were first alternately...
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| Veröffentlicht in: | RSC advances 2015-01, Vol.5 (8), p.5988-5995 |
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| creator | Zhao, Yue Zhou, Changhua Wu, Ruili Li, Lin Shen, Huaibin Li, Lin Song |
| description | In this paper, we reported the preparation of carboxyl functionalized quantum dots (QDs)-embedded silica nanoparticles by combining layer-by-layer (LbL) self-assembly technique and a multi-layer protection method. Preformed SiO
2
nanoparticles synthesized
via
the Stöber method were first alternately deposited with positively charged polyelectrolyte (poly(diallyl dimethylammonium chloride), PDDA) and negatively charged CdSe/ZnS QDs by LBL method and further coated by a silica shell. Then, the functionalization of SiO
2
@PDDA@QDs@SiO
2
nanoparticles with hydrophobic hydrocarbons and biocompatible oligomers impart optical imaging properties and good colloidal stability in aqueous solutions at a wide range of pH, PBS buffer, and under thermal treatment. In addition, these newly developed multi-shell structured nanoparticles coupled with specific antibodies were successfully applied in detecting procalcitonin (PCT) antigens using fluorescent lateral flow immunoassay (LFIA). The naked-eye detection limit of fluorescence nanoparticles could achieve 0.1 ng mL
−1
of PCT antigens, which was about two hundred times higher than that of colloidal gold-based method. |
| doi_str_mv | 10.1039/C4RA13362E |
| format | Article |
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2
nanoparticles synthesized
via
the Stöber method were first alternately deposited with positively charged polyelectrolyte (poly(diallyl dimethylammonium chloride), PDDA) and negatively charged CdSe/ZnS QDs by LBL method and further coated by a silica shell. Then, the functionalization of SiO
2
@PDDA@QDs@SiO
2
nanoparticles with hydrophobic hydrocarbons and biocompatible oligomers impart optical imaging properties and good colloidal stability in aqueous solutions at a wide range of pH, PBS buffer, and under thermal treatment. In addition, these newly developed multi-shell structured nanoparticles coupled with specific antibodies were successfully applied in detecting procalcitonin (PCT) antigens using fluorescent lateral flow immunoassay (LFIA). The naked-eye detection limit of fluorescence nanoparticles could achieve 0.1 ng mL
−1
of PCT antigens, which was about two hundred times higher than that of colloidal gold-based method.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/C4RA13362E</identifier><language>eng</language><subject>Antigens ; Chlorides ; Colloids ; Fluorescence ; Nanoparticles ; Quantum dots ; Self assembly ; Silicon dioxide</subject><ispartof>RSC advances, 2015-01, Vol.5 (8), p.5988-5995</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-b121e872f13d0f98a06a98ef49da8951fe825bcb114f56e0c729a90e7ec113983</citedby><cites>FETCH-LOGICAL-c330t-b121e872f13d0f98a06a98ef49da8951fe825bcb114f56e0c729a90e7ec113983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Zhao, Yue</creatorcontrib><creatorcontrib>Zhou, Changhua</creatorcontrib><creatorcontrib>Wu, Ruili</creatorcontrib><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Shen, Huaibin</creatorcontrib><creatorcontrib>Li, Lin Song</creatorcontrib><title>Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection</title><title>RSC advances</title><description>In this paper, we reported the preparation of carboxyl functionalized quantum dots (QDs)-embedded silica nanoparticles by combining layer-by-layer (LbL) self-assembly technique and a multi-layer protection method. Preformed SiO
2
nanoparticles synthesized
via
the Stöber method were first alternately deposited with positively charged polyelectrolyte (poly(diallyl dimethylammonium chloride), PDDA) and negatively charged CdSe/ZnS QDs by LBL method and further coated by a silica shell. Then, the functionalization of SiO
2
@PDDA@QDs@SiO
2
nanoparticles with hydrophobic hydrocarbons and biocompatible oligomers impart optical imaging properties and good colloidal stability in aqueous solutions at a wide range of pH, PBS buffer, and under thermal treatment. In addition, these newly developed multi-shell structured nanoparticles coupled with specific antibodies were successfully applied in detecting procalcitonin (PCT) antigens using fluorescent lateral flow immunoassay (LFIA). The naked-eye detection limit of fluorescence nanoparticles could achieve 0.1 ng mL
−1
of PCT antigens, which was about two hundred times higher than that of colloidal gold-based method.</description><subject>Antigens</subject><subject>Chlorides</subject><subject>Colloids</subject><subject>Fluorescence</subject><subject>Nanoparticles</subject><subject>Quantum dots</subject><subject>Self assembly</subject><subject>Silicon dioxide</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpNUMtKBDEQDKLgsu7FL8hRhNE85pEcl2V9wIIieh4ymQ4bySRjkhH0651lBe1LN1RVd1chdEnJDSVc3m7KlzXlvGbbE7RgpKwLRmp5-m8-R6uU3slcdUVZTRfo-znCqKLKNngcDB4ml22R9uAcTjlOOk8RemzcFCIkDT5jHYYxJJsBe-XDLM5WO0jYhIhndVQJ_AzbT8D7aVAejzFo5bTNwVuPe8igD-cu0JlRLsHqty_R2932dfNQ7J7uHzfrXaE5J7noKKMgGmYo74mRQpFaSQGmlL0SsqIGBKs63VFamqoGohsmlSTQgKaUS8GX6Oq4d_7jY4KU28HOTpxTHsKUWirKShDeNOVMvT5SdQwpRTDtGO2g4ldLSXvIuP3LmP8A1CZyJg</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Zhao, Yue</creator><creator>Zhou, Changhua</creator><creator>Wu, Ruili</creator><creator>Li, Lin</creator><creator>Shen, Huaibin</creator><creator>Li, Lin Song</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20150101</creationdate><title>Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection</title><author>Zhao, Yue ; Zhou, Changhua ; Wu, Ruili ; Li, Lin ; Shen, Huaibin ; Li, Lin Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-b121e872f13d0f98a06a98ef49da8951fe825bcb114f56e0c729a90e7ec113983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antigens</topic><topic>Chlorides</topic><topic>Colloids</topic><topic>Fluorescence</topic><topic>Nanoparticles</topic><topic>Quantum dots</topic><topic>Self assembly</topic><topic>Silicon dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yue</creatorcontrib><creatorcontrib>Zhou, Changhua</creatorcontrib><creatorcontrib>Wu, Ruili</creatorcontrib><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Shen, Huaibin</creatorcontrib><creatorcontrib>Li, Lin Song</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yue</au><au>Zhou, Changhua</au><au>Wu, Ruili</au><au>Li, Lin</au><au>Shen, Huaibin</au><au>Li, Lin Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>8</issue><spage>5988</spage><epage>5995</epage><pages>5988-5995</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>In this paper, we reported the preparation of carboxyl functionalized quantum dots (QDs)-embedded silica nanoparticles by combining layer-by-layer (LbL) self-assembly technique and a multi-layer protection method. Preformed SiO
2
nanoparticles synthesized
via
the Stöber method were first alternately deposited with positively charged polyelectrolyte (poly(diallyl dimethylammonium chloride), PDDA) and negatively charged CdSe/ZnS QDs by LBL method and further coated by a silica shell. Then, the functionalization of SiO
2
@PDDA@QDs@SiO
2
nanoparticles with hydrophobic hydrocarbons and biocompatible oligomers impart optical imaging properties and good colloidal stability in aqueous solutions at a wide range of pH, PBS buffer, and under thermal treatment. In addition, these newly developed multi-shell structured nanoparticles coupled with specific antibodies were successfully applied in detecting procalcitonin (PCT) antigens using fluorescent lateral flow immunoassay (LFIA). The naked-eye detection limit of fluorescence nanoparticles could achieve 0.1 ng mL
−1
of PCT antigens, which was about two hundred times higher than that of colloidal gold-based method.</abstract><doi>10.1039/C4RA13362E</doi><tpages>8</tpages></addata></record> |
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| ispartof | RSC advances, 2015-01, Vol.5 (8), p.5988-5995 |
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| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Antigens Chlorides Colloids Fluorescence Nanoparticles Quantum dots Self assembly Silicon dioxide |
| title | Preparation of multi-shell structured fluorescent composite nanoparticles for ultrasensitive human procalcitonin detection |
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