Polyurea dendrimer for efficient cytosolic siRNA delivery
The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bondin...
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| Veröffentlicht in: | RSC advances 2014-01, Vol.4 (97), p.54872-54878 |
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| container_issue | 97 |
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| container_title | RSC advances |
| container_volume | 4 |
| creator | Restani, Rita B Conde, Joao Baptista, Pedro V Cidade, Maria Teresa Braganca, Ana M Morgado, Jorge Correia, Ilidio J Aguiar-Ricardo, Ana Bonifacio, Vasco DB |
| description | The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery. |
| doi_str_mv | 10.1039/c4ra09603g |
| format | Article |
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| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2014-01, Vol.4 (97), p.54872-54878 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_1845800035 |
| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Amines Biocompatibility Dendrimers Effectiveness Nanostructure Platforms Polyureas Toxicity |
| title | Polyurea dendrimer for efficient cytosolic siRNA delivery |
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