MALDI Imaging of Liquid Extraction Surface Analysis Sampled Tissue
Combined mass spectrometry imaging methods in which two different techniques are executed on the same sample have recently been reported for a number of sample types. Such an approach can be used to examine the sampling effects of the first technique with a second, higher resolution method and also...
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Veröffentlicht in: | Analytical chemistry (Washington) 2016-09, Vol.88 (17), p.8433-8440 |
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creator | Randall, Elizabeth C Race, Alan M Cooper, Helen J Bunch, Josephine |
description | Combined mass spectrometry imaging methods in which two different techniques are executed on the same sample have recently been reported for a number of sample types. Such an approach can be used to examine the sampling effects of the first technique with a second, higher resolution method and also combines the advantages of each technique for a more complete analysis. In this work matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) was used to study the effects of liquid extraction surface analysis (LESA) sampling on mouse brain tissue. Complementary multivariate analysis techniques including principal component analysis, non-negative matrix factorization, and t-distributed stochastic neighbor embedding were applied to MALDI MS images acquired from tissue which had been sampled by LESA to gain a better understanding of localized tissue washing in LESA sampling. It was found that MALDI MS images could be used to visualize regions sampled by LESA. The variability in sampling area, spatial precision, and delocalization of analytes in tissue induced by LESA were assessed using both single-ion images and images provided by multivariate analysis. |
doi_str_mv | 10.1021/acs.analchem.5b04281 |
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Chem</addtitle><description>Combined mass spectrometry imaging methods in which two different techniques are executed on the same sample have recently been reported for a number of sample types. Such an approach can be used to examine the sampling effects of the first technique with a second, higher resolution method and also combines the advantages of each technique for a more complete analysis. In this work matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) was used to study the effects of liquid extraction surface analysis (LESA) sampling on mouse brain tissue. Complementary multivariate analysis techniques including principal component analysis, non-negative matrix factorization, and t-distributed stochastic neighbor embedding were applied to MALDI MS images acquired from tissue which had been sampled by LESA to gain a better understanding of localized tissue washing in LESA sampling. It was found that MALDI MS images could be used to visualize regions sampled by LESA. The variability in sampling area, spatial precision, and delocalization of analytes in tissue induced by LESA were assessed using both single-ion images and images provided by multivariate analysis.</description><subject>Animals</subject><subject>Brain Chemistry</subject><subject>Extraction</subject><subject>Imaging</subject><subject>Ionization</subject><subject>Liquid-Liquid Extraction</subject><subject>Liquids</subject><subject>Mass spectrometry</subject><subject>Matrix</subject><subject>Mice</subject><subject>Multivariate Analysis</subject><subject>Principal components analysis</subject><subject>Rodents</subject><subject>Sampling</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Surface Properties</subject><subject>Tissues</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1OwzAQhC0EoqXwBghF4sIlZdeJneRYSoFKRRxazpHj2MVVfkqcSPTtcWkLEgfEaS_fzGhnCLlEGCJQvBXSDkUlCvmmyiHLIKQxHpE-Mgo-j2N6TPoAEPg0AuiRM2tXAIiA_JT0aBSGkTPpk7vn0ex-6k1LsTTV0qu1NzPvncm9yUfbCNmauvLmXaOFVN7IpW2ssd5clOtC5d7CWNupc3KiRWHVxf4OyOvDZDF-8mcvj9PxaOYLRrH1udQYJVJTneU8C3IKVHAOAhjTSR5EkiLPMYlBoCOyQCLGWnKmQ-SgWBIMyM3Od93U752ybVoaK1VRiErVnU0xDlkUJyGl_0AxYhwpBA69_oWu6q5xn35RSRgkrkBHhTtKNrW1jdLpujGlaDYpQrqdI3VzpIc50v0cTna1N--yUuXfokP_DoAdsJX_BP_l-QnERpbO</recordid><startdate>20160906</startdate><enddate>20160906</enddate><creator>Randall, Elizabeth C</creator><creator>Race, Alan M</creator><creator>Cooper, Helen J</creator><creator>Bunch, Josephine</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20160906</creationdate><title>MALDI Imaging of Liquid Extraction Surface Analysis Sampled Tissue</title><author>Randall, Elizabeth C ; 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subjects | Animals Brain Chemistry Extraction Imaging Ionization Liquid-Liquid Extraction Liquids Mass spectrometry Matrix Mice Multivariate Analysis Principal components analysis Rodents Sampling Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surface Properties Tissues |
title | MALDI Imaging of Liquid Extraction Surface Analysis Sampled Tissue |
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