Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess
The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella...
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| Veröffentlicht in: | Journal of medical microbiology 2017-01, Vol.66 (1), p.1-7 |
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| creator | Ou, Qin Fan, Jinming Duan, Dejian Xu, Li Wang, Jie Zhou, Dongsheng Yang, Huiying Li, Bei |
| description | The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella pneumoniae capsular serotype K1 strain causing the primary pyogenic liver abscess. The phenotypes of wild-type strain, crp mutant and complemented mutant were characterized systematically. It was disclosed that K. pneumoniae CRP was required for the in vitro growth, fimbria production, biofilm formation and lethality in mouse, but it inhibited the capsular polysaccharide biosynthesis. These indicated the important roles of CRP in regulating the expression of virulence and biofilm genes in K. pneumoniae. |
| doi_str_mv | 10.1099/jmm.0.000391 |
| format | Article |
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These indicated the important roles of CRP in regulating the expression of virulence and biofilm genes in K. pneumoniae.</description><subject>Animals</subject><subject>Bacterial Capsules - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms - growth & development</subject><subject>Cyclic AMP Receptor Protein - genetics</subject><subject>Cyclic AMP Receptor Protein - metabolism</subject><subject>Female</subject><subject>Fimbriae, Bacterial - metabolism</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Klebsiella pneumoniae - genetics</subject><subject>Klebsiella pneumoniae - metabolism</subject><subject>Klebsiella pneumoniae - pathogenicity</subject><subject>Liver Abscess, Pyogenic - microbiology</subject><subject>Liver Abscess, Pyogenic - pathology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Polysaccharides, Bacterial</subject><subject>RNA, Bacterial - genetics</subject><subject>Serotyping</subject><subject>Virulence</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtr3DAUhUVpaKZpd10XLbuIJ1ey_FqGkLYhKc0iXRtZvsoo6OFK9oD_ZH9TZSYtEgjEd885l0PIJwZ7Bl139eLcHvYAUHbsDdkx0ZRFVQvxluwAOC94zapz8j6lFwDWlGX3jpzzpgMugO3Inzt_DPaIDv1Mg6bq-scjjahwmkOkUwwzGk_zHUzQxjqqQ3RyNsFfUm3cEI3cqHFRpz8lp7RYmUeDXZNU6iCjGXEbT6ufD5hMotKP1OJ8kNbM6ybuwpJws7-3OCSD1mZVj4sL3kikCXOOdUJ6z7LBkox_ptMantEbRa05YqRySApT-kDOtLQJP76-F-TX19unm-_Fw89vdzfXD4Uqu2ou1AhCNrptpawFr6EaNbZKVDofxhqEsgQJIJqq5goHqDqtahw7xHZo-CDLC_LlpJt3_71gmntncoCc22PepWetqHiVvVhGL0-oiiGliLqfonEyrj2Dfmuwzw320J8azPjnV-VlcDj-h_9VVv4FtECdaA</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Ou, Qin</creator><creator>Fan, Jinming</creator><creator>Duan, Dejian</creator><creator>Xu, Li</creator><creator>Wang, Jie</creator><creator>Zhou, Dongsheng</creator><creator>Yang, Huiying</creator><creator>Li, Bei</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess</title><author>Ou, Qin ; Fan, Jinming ; Duan, Dejian ; Xu, Li ; Wang, Jie ; Zhou, Dongsheng ; Yang, Huiying ; Li, Bei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-cd04a7f88aa642605dfe8c45f5f5117e0330a0047562ceb059fc6ed9ee8b72ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bacterial Capsules - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms - growth & development</topic><topic>Cyclic AMP Receptor Protein - genetics</topic><topic>Cyclic AMP Receptor Protein - metabolism</topic><topic>Female</topic><topic>Fimbriae, Bacterial - metabolism</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Klebsiella pneumoniae - genetics</topic><topic>Klebsiella pneumoniae - metabolism</topic><topic>Klebsiella pneumoniae - pathogenicity</topic><topic>Liver Abscess, Pyogenic - microbiology</topic><topic>Liver Abscess, Pyogenic - pathology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Polysaccharides, Bacterial</topic><topic>RNA, Bacterial - genetics</topic><topic>Serotyping</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ou, Qin</creatorcontrib><creatorcontrib>Fan, Jinming</creatorcontrib><creatorcontrib>Duan, Dejian</creatorcontrib><creatorcontrib>Xu, Li</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Zhou, Dongsheng</creatorcontrib><creatorcontrib>Yang, Huiying</creatorcontrib><creatorcontrib>Li, Bei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ou, Qin</au><au>Fan, Jinming</au><au>Duan, Dejian</au><au>Xu, Li</au><au>Wang, Jie</au><au>Zhou, Dongsheng</au><au>Yang, Huiying</au><au>Li, Bei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2017-01</date><risdate>2017</risdate><volume>66</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><abstract>The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella pneumoniae capsular serotype K1 strain causing the primary pyogenic liver abscess. The phenotypes of wild-type strain, crp mutant and complemented mutant were characterized systematically. It was disclosed that K. pneumoniae CRP was required for the in vitro growth, fimbria production, biofilm formation and lethality in mouse, but it inhibited the capsular polysaccharide biosynthesis. These indicated the important roles of CRP in regulating the expression of virulence and biofilm genes in K. pneumoniae.</abstract><cop>England</cop><pmid>27902401</pmid><doi>10.1099/jmm.0.000391</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Microbiology Society; Alma/SFX Local Collection |
| subjects | Animals Bacterial Capsules - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms - growth & development Cyclic AMP Receptor Protein - genetics Cyclic AMP Receptor Protein - metabolism Female Fimbriae, Bacterial - metabolism Gene Deletion Gene Expression Regulation, Bacterial Klebsiella pneumoniae - genetics Klebsiella pneumoniae - metabolism Klebsiella pneumoniae - pathogenicity Liver Abscess, Pyogenic - microbiology Liver Abscess, Pyogenic - pathology Mice Mice, Inbred BALB C Polysaccharides, Bacterial RNA, Bacterial - genetics Serotyping Virulence |
| title | Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess |
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