Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics
Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts we...
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| Veröffentlicht in: | Transgenic research 2017-02, Vol.26 (1), p.77-85 |
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| creator | Shi, Bingbo Ding, Qiang He, Xiaolin Zhu, Haijing Niu, Yiyuan Cai, Bei Cai, Jiao Lei, Anming Kang, Danju Yan, Hailong Ma, Baohua Wang, Xiaolong Qu, Lei Chen, Yulin |
| description | Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (
Tβ4
) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (
KAP6.1
) promoter. To obtain genetically modified cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts of cashmere goats. Five transgenic cashmere goats were generated following somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and integration sites, the
Tβ4
gene was successfully inserted into the goat genome. Histological examination of skin tissue revealed that
Tβ4
-overexpressing, transgenic goats had a higher secondary to primary hair follicle (S/P) ratio compared to wild type goats. This indicates that
Tβ4
-overexpressing goats possess increased numbers of secondary hair follicles (SHF). Our results indicate that
Tβ4
-overexpression in cashmere goats could be a feasible strategy to increase cashmere yield. |
| doi_str_mv | 10.1007/s11248-016-9988-7 |
| format | Article |
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Tβ4
) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (
KAP6.1
) promoter. To obtain genetically modified cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts of cashmere goats. Five transgenic cashmere goats were generated following somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and integration sites, the
Tβ4
gene was successfully inserted into the goat genome. Histological examination of skin tissue revealed that
Tβ4
-overexpressing, transgenic goats had a higher secondary to primary hair follicle (S/P) ratio compared to wild type goats. This indicates that
Tβ4
-overexpressing goats possess increased numbers of secondary hair follicles (SHF). Our results indicate that
Tβ4
-overexpression in cashmere goats could be a feasible strategy to increase cashmere yield.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-016-9988-7</identifier><identifier>PMID: 27900536</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Genetics and Genomics ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - growth & development ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; DNA Transposable Elements - genetics ; Fibroblasts - metabolism ; Gene Expression Regulation ; Genetic Engineering ; Goats - genetics ; Goats - growth & development ; Hair Follicle - growth & development ; Hair Follicle - metabolism ; Keratins - genetics ; Keratins - metabolism ; Life Sciences ; Molecular Medicine ; Nuclear Transfer Techniques ; Original Paper ; Plant Genetics and Genomics ; Skin - growth & development ; Skin - metabolism ; Thymosin - genetics ; Thymosin - metabolism ; Transgenics</subject><ispartof>Transgenic research, 2017-02, Vol.26 (1), p.77-85</ispartof><rights>Springer International Publishing Switzerland 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2597-34d2257d55da52da778f54261534b6e52e8b1e3861b1d8642d458a0e0c1dc1ae3</citedby><cites>FETCH-LOGICAL-c2597-34d2257d55da52da778f54261534b6e52e8b1e3861b1d8642d458a0e0c1dc1ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-016-9988-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-016-9988-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27926,27927,41490,42559,51321</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27900536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Bingbo</creatorcontrib><creatorcontrib>Ding, Qiang</creatorcontrib><creatorcontrib>He, Xiaolin</creatorcontrib><creatorcontrib>Zhu, Haijing</creatorcontrib><creatorcontrib>Niu, Yiyuan</creatorcontrib><creatorcontrib>Cai, Bei</creatorcontrib><creatorcontrib>Cai, Jiao</creatorcontrib><creatorcontrib>Lei, Anming</creatorcontrib><creatorcontrib>Kang, Danju</creatorcontrib><creatorcontrib>Yan, Hailong</creatorcontrib><creatorcontrib>Ma, Baohua</creatorcontrib><creatorcontrib>Wang, Xiaolong</creatorcontrib><creatorcontrib>Qu, Lei</creatorcontrib><creatorcontrib>Chen, Yulin</creatorcontrib><title>Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (
Tβ4
) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (
KAP6.1
) promoter. To obtain genetically modified cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts of cashmere goats. Five transgenic cashmere goats were generated following somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and integration sites, the
Tβ4
gene was successfully inserted into the goat genome. Histological examination of skin tissue revealed that
Tβ4
-overexpressing, transgenic goats had a higher secondary to primary hair follicle (S/P) ratio compared to wild type goats. This indicates that
Tβ4
-overexpressing goats possess increased numbers of secondary hair follicles (SHF). Our results indicate that
Tβ4
-overexpression in cashmere goats could be a feasible strategy to increase cashmere yield.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - growth & development</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>DNA Transposable Elements - genetics</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Genetic Engineering</subject><subject>Goats - genetics</subject><subject>Goats - growth & development</subject><subject>Hair Follicle - growth & development</subject><subject>Hair Follicle - metabolism</subject><subject>Keratins - genetics</subject><subject>Keratins - metabolism</subject><subject>Life Sciences</subject><subject>Molecular Medicine</subject><subject>Nuclear Transfer Techniques</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Skin - growth & development</subject><subject>Skin - metabolism</subject><subject>Thymosin - genetics</subject><subject>Thymosin - metabolism</subject><subject>Transgenics</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OHDEQha0IFAbIAbKJvGTjxD_ttnuZICBISGxgbbntmhmPptsdV0-UuRYH4Ux4NCTLrKr06r0n1UfIZ8G_Cs7NNxRCNpZx0bKus5aZD2QhtFGsU609IQvetZJZK7ozco644bymrPpIzqTpONeqXZD89PrSsPwbCvyZCiCmPNLeI0Ral3kNdEqr1f6HD3QufsQpY9VxjzMMNI00eFwPNUxX2c9I_XaGgnTtU6HL1EOhYe2LD1VNOKeAl-R06bcIn97nBXm-vXm6_skeHu_ur78_sCB1Z5hqopTaRK2j1zJ6Y-xSN7IVWjV9C1qC7QUo24peRNs2Mjbaeg48iBiEB3VBro69U8m_doCzGxIG2G79CHmHTthGV3bGqmoVR2soGbHA0k0lDb7sneDuwNkdObvK2R04O1MzX97rd_0A8V_iL9hqkEcD1tO4guI2eVfG-vJ_Wt8AOwCKfw</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Shi, Bingbo</creator><creator>Ding, Qiang</creator><creator>He, Xiaolin</creator><creator>Zhu, Haijing</creator><creator>Niu, Yiyuan</creator><creator>Cai, Bei</creator><creator>Cai, Jiao</creator><creator>Lei, Anming</creator><creator>Kang, Danju</creator><creator>Yan, Hailong</creator><creator>Ma, Baohua</creator><creator>Wang, Xiaolong</creator><creator>Qu, Lei</creator><creator>Chen, Yulin</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics</title><author>Shi, Bingbo ; Ding, Qiang ; He, Xiaolin ; Zhu, Haijing ; Niu, Yiyuan ; Cai, Bei ; Cai, Jiao ; Lei, Anming ; Kang, Danju ; Yan, Hailong ; Ma, Baohua ; Wang, Xiaolong ; Qu, Lei ; Chen, Yulin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2597-34d2257d55da52da778f54261534b6e52e8b1e3861b1d8642d458a0e0c1dc1ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Animals, Genetically Modified - genetics</topic><topic>Animals, Genetically Modified - growth & development</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>DNA Transposable Elements - genetics</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Genetic Engineering</topic><topic>Goats - genetics</topic><topic>Goats - growth & development</topic><topic>Hair Follicle - growth & development</topic><topic>Hair Follicle - metabolism</topic><topic>Keratins - genetics</topic><topic>Keratins - metabolism</topic><topic>Life Sciences</topic><topic>Molecular Medicine</topic><topic>Nuclear Transfer Techniques</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Skin - growth & development</topic><topic>Skin - metabolism</topic><topic>Thymosin - genetics</topic><topic>Thymosin - metabolism</topic><topic>Transgenics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Bingbo</creatorcontrib><creatorcontrib>Ding, Qiang</creatorcontrib><creatorcontrib>He, Xiaolin</creatorcontrib><creatorcontrib>Zhu, Haijing</creatorcontrib><creatorcontrib>Niu, Yiyuan</creatorcontrib><creatorcontrib>Cai, Bei</creatorcontrib><creatorcontrib>Cai, Jiao</creatorcontrib><creatorcontrib>Lei, Anming</creatorcontrib><creatorcontrib>Kang, Danju</creatorcontrib><creatorcontrib>Yan, Hailong</creatorcontrib><creatorcontrib>Ma, Baohua</creatorcontrib><creatorcontrib>Wang, Xiaolong</creatorcontrib><creatorcontrib>Qu, Lei</creatorcontrib><creatorcontrib>Chen, Yulin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Bingbo</au><au>Ding, Qiang</au><au>He, Xiaolin</au><au>Zhu, Haijing</au><au>Niu, Yiyuan</au><au>Cai, Bei</au><au>Cai, Jiao</au><au>Lei, Anming</au><au>Kang, Danju</au><au>Yan, Hailong</au><au>Ma, Baohua</au><au>Wang, Xiaolong</au><au>Qu, Lei</au><au>Chen, Yulin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>26</volume><issue>1</issue><spage>77</spage><epage>85</epage><pages>77-85</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (
Tβ4
) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (
KAP6.1
) promoter. To obtain genetically modified cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts of cashmere goats. Five transgenic cashmere goats were generated following somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and integration sites, the
Tβ4
gene was successfully inserted into the goat genome. Histological examination of skin tissue revealed that
Tβ4
-overexpressing, transgenic goats had a higher secondary to primary hair follicle (S/P) ratio compared to wild type goats. This indicates that
Tβ4
-overexpressing goats possess increased numbers of secondary hair follicles (SHF). Our results indicate that
Tβ4
-overexpression in cashmere goats could be a feasible strategy to increase cashmere yield.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>27900536</pmid><doi>10.1007/s11248-016-9988-7</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0962-8819 |
| ispartof | Transgenic research, 2017-02, Vol.26 (1), p.77-85 |
| issn | 0962-8819 1573-9368 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animal Genetics and Genomics Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - growth & development Biomedical and Life Sciences Biomedical Engineering/Biotechnology DNA Transposable Elements - genetics Fibroblasts - metabolism Gene Expression Regulation Genetic Engineering Goats - genetics Goats - growth & development Hair Follicle - growth & development Hair Follicle - metabolism Keratins - genetics Keratins - metabolism Life Sciences Molecular Medicine Nuclear Transfer Techniques Original Paper Plant Genetics and Genomics Skin - growth & development Skin - metabolism Thymosin - genetics Thymosin - metabolism Transgenics |
| title | Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics |
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