Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine
Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine “Zuoshan-1,” a clonal selection from wild Vitis amurensis Rupr. The predicted thaumatin-like protein (VaTLP) h...
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| Veröffentlicht in: | Protoplasma 2017-07, Vol.254 (4), p.1579-1589 |
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| creator | He, Rongrong Wu, Jiao Zhang, Yali Agüero, Cecilia B. Li, Xinlong Liu, Shaoli Wang, Chaoxia Walker, M. Andrew Lu, Jiang |
| description | Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine “Zuoshan-1,” a clonal selection from wild
Vitis amurensis
Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of
V. vinifera
‘Thompson Seedless’ via
Agrobacterium tumefaciens
. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with
Plasmopara viticola
, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to
P. viticola
in transgenic ‘Thompson Seedless’ grapevines. |
| doi_str_mv | 10.1007/s00709-016-1047-y |
| format | Article |
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Vitis amurensis
Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of
V. vinifera
‘Thompson Seedless’ via
Agrobacterium tumefaciens
. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with
Plasmopara viticola
, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to
P. viticola
in transgenic ‘Thompson Seedless’ grapevines.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-016-1047-y</identifier><identifier>PMID: 27900595</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Asexual reproduction ; Biomedical and Life Sciences ; Cell Biology ; Clonal selection ; Disease Resistance ; Downy mildew ; Gene Expression ; Genetic Enhancement ; Harvest ; Hyphae ; Hyphae - growth & development ; Leaves ; Life Sciences ; Oomycetes - growth & development ; Original Article ; Pathogens ; Plant diseases ; Plant Diseases - microbiology ; Plant Leaves - genetics ; Plant Leaves - microbiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants, Genetically Modified - genetics ; Polymerase chain reaction ; Proteins ; Thaumatin ; Transcriptional Activation ; Transgenic plants ; Vitis - genetics ; Vitis - microbiology ; Zoology</subject><ispartof>Protoplasma, 2017-07, Vol.254 (4), p.1579-1589</ispartof><rights>Springer-Verlag Wien 2016</rights><rights>Protoplasma is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-259286fc37842f7e19e796e4f7559d9069448bd2fc6c992cc8510ced66c1dbfa3</citedby><cites>FETCH-LOGICAL-c438t-259286fc37842f7e19e796e4f7559d9069448bd2fc6c992cc8510ced66c1dbfa3</cites><orcidid>0000-0001-9872-7598</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-016-1047-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-016-1047-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27900595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Rongrong</creatorcontrib><creatorcontrib>Wu, Jiao</creatorcontrib><creatorcontrib>Zhang, Yali</creatorcontrib><creatorcontrib>Agüero, Cecilia B.</creatorcontrib><creatorcontrib>Li, Xinlong</creatorcontrib><creatorcontrib>Liu, Shaoli</creatorcontrib><creatorcontrib>Wang, Chaoxia</creatorcontrib><creatorcontrib>Walker, M. Andrew</creatorcontrib><creatorcontrib>Lu, Jiang</creatorcontrib><title>Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine “Zuoshan-1,” a clonal selection from wild
Vitis amurensis
Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of
V. vinifera
‘Thompson Seedless’ via
Agrobacterium tumefaciens
. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with
Plasmopara viticola
, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to
P. viticola
in transgenic ‘Thompson Seedless’ grapevines.</description><subject>Asexual reproduction</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Clonal selection</subject><subject>Disease Resistance</subject><subject>Downy mildew</subject><subject>Gene Expression</subject><subject>Genetic Enhancement</subject><subject>Harvest</subject><subject>Hyphae</subject><subject>Hyphae - growth & development</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Oomycetes - growth & development</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - microbiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Thaumatin</subject><subject>Transcriptional Activation</subject><subject>Transgenic plants</subject><subject>Vitis - genetics</subject><subject>Vitis - microbiology</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU9rFTEUxYNU7LP6AdyUQDfdxObPJJMsS9EqFLqp4i7kZW6eaWcyr8nMq2_pNzdlapGCWST3kt8598JB6AOjHxml7VmpFzWEMkUYbVqyf4VWTDFJFKP8AK0oFYIwLX4corel3FJKJafyDTrkram1kSv0-3oHGX5tM5QSx4THgB2efrp5cFNMpI93gLd5nCAmvIEEOORxwN_jFAt2w5whlVrFoTI7KLgbH9IeD7Hv4AFXz1gmlzzgql40u5higOzwJrst1A7eodfB9QXeP71H6NvnTzcXX8jV9eXXi_Mr4huhJ8Kl4VoFL1rd8NACM9AaBU1opTSdoco0jV53PHjljeHea8moh04pz7p1cOIInS6-ddX7Gcpkh1g89L1LMM7FMt1I3mghdEVPXqC345xT3c4yw-oRUrBKsYXyeSwlQ7DbHAeX95ZR-5iPXfKxNR_7mI_dV83xk_O8HqB7VvwNpAJ8AUr9ShvI_4z-r-sfRROd4Q</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>He, Rongrong</creator><creator>Wu, Jiao</creator><creator>Zhang, Yali</creator><creator>Agüero, Cecilia B.</creator><creator>Li, Xinlong</creator><creator>Liu, Shaoli</creator><creator>Wang, Chaoxia</creator><creator>Walker, M. 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Andrew</au><au>Lu, Jiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>254</volume><issue>4</issue><spage>1579</spage><epage>1589</epage><pages>1579-1589</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine “Zuoshan-1,” a clonal selection from wild
Vitis amurensis
Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of
V. vinifera
‘Thompson Seedless’ via
Agrobacterium tumefaciens
. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with
Plasmopara viticola
, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to
P. viticola
in transgenic ‘Thompson Seedless’ grapevines.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>27900595</pmid><doi>10.1007/s00709-016-1047-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-9872-7598</orcidid></addata></record> |
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| ispartof | Protoplasma, 2017-07, Vol.254 (4), p.1579-1589 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Asexual reproduction Biomedical and Life Sciences Cell Biology Clonal selection Disease Resistance Downy mildew Gene Expression Genetic Enhancement Harvest Hyphae Hyphae - growth & development Leaves Life Sciences Oomycetes - growth & development Original Article Pathogens Plant diseases Plant Diseases - microbiology Plant Leaves - genetics Plant Leaves - microbiology Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified - genetics Polymerase chain reaction Proteins Thaumatin Transcriptional Activation Transgenic plants Vitis - genetics Vitis - microbiology Zoology |
| title | Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine |
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